KIAA0319 (NM_001252328) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AAVR; DYLX2; DYX2; NMIG
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238490 representing NM_001252328 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAAGGAGATTATACATTTCAGCTGAAGGTGACAGATTCTTCAAGGCAACAGTCTACTGCTGTGG TGACTGTGATTGTCCAGCCTGAAAACAATAGACCTCCAGTGGCTGTGGCCGGCCCTGATAAAGAGCTGAT CTTCCCAGTGGAAAGTGCTACCCTGGATGGGAGCAGCAGCAGCGATGACCACGGCATTGTCTTCTACCAC TGGGAGCACGTCAGAGGCCCCAGTGCAGTGGAGATGGAAAATATTGACAAAGCAATAGCCACTGTGACTG GTCTCCAGGTGGGGACCTACCACTTCCGTTTGACAGTGAAAGACCAGCAGGGACTGAGCAGCACGTCCAC CCTCACTGTGGCTGTGAAGAAGGAAAATAATAGTCCTCCCAGAGCCCGGGCTGGTGGCAGACATGTTCTT GTGCTTCCCAATAATTCCATTACTTTGGATGGTTCAAGGTCTACTGATGACCAAAGAATTGTGTCCTATC TGTGGATCCGGGATGGCCAGAGTCCAGCAGCTGGAGATGTCATCGATGGCTCTGACCACAGTGTGGCTCT GCAGCTTACGAATCTGGTGGAGGGGGTGTACACTTTCCACTTGCGAGTCACCGACAGTCAGGGGGCCTCG GACACAGACACTGCCACTGTGGAAGTGCAGCCAGACCCTAGGAAGAGTGGCCTGGTGGAGCTGACCCTGC AGGTTGGTGTTGGGCAGCTGACAGAGCAGCGGAAGGACACCCTTGTGAGGCAGCTGGCTGTGCTGCTGAA CGTGCTGGACTCGGACATTAAGGTCCAGAAGATTCGGGCCCACTCGGATCTCAGCACCGTGATTGTGTTT TATGTACAGAGCAGGCCGCCTTTCAAGGTTCTCAAAGCTGCTGAAGTGGCCCGAAATCTGCACATGCGGC TCTCAAAGGAGAAGGCTGACTTCTTGCTTTTCAAGGTCTTGAGGGTTGATACAGCAGGTTGCCTTCTGAA GTGTTCTGGCCATGGTCACTGCGACCCCCTCACAAAGCGCTGCATTTGCTCTCACTTATGGATGGAGAAC CTTATACAGCGTTATATCTGGGATGGAGAGAGCAACTGTGAGTGGAGTATATTCTATGTGACAGTGTTGG CTTTTACTCTTATTGTGCTAACAGGAGGTTTCACTTGGCTTTGCATCTGCTGCTGCAAAAGACAAAAAAG GACTAAAATCAGGAAAAAAACAAAGTACACCATCCTGGATAACATGGATGAACAGGAAAGAATGGAACTG AGGCCCAAATATGGTATCAAGCACCGAAGCACAGAGCACAACTCCAGCCTGATGGTATCCGAGTCTGAGT TTGACAGTGACCAGGACACAATCTTCAGCCGAGAAAAGATGGAGAGAGGGAATCCAAAGGTTTCCATGAA TGGTTCCATCAGAAATGGAGCTTCCTTCAGTTATTGCTCAAAGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238490 representing NM_001252328 Red=Cloning site Green=Tags(s) MQEGDYTFQLKVTDSSRQQSTAVVTVIVQPENNRPPVAVAGPDKELIFPVESATLDGSSSSDDHGIVFYH WEHVRGPSAVEMENIDKAIATVTGLQVGTYHFRLTVKDQQGLSSTSTLTVAVKKENNSPPRARAGGRHVL VLPNNSITLDGSRSTDDQRIVSYLWIRDGQSPAAGDVIDGSDHSVALQLTNLVEGVYTFHLRVTDSQGAS DTDTATVEVQPDPRKSGLVELTLQVGVGQLTEQRKDTLVRQLAVLLNVLDSDIKVQKIRAHSDLSTVIVF YVQSRPPFKVLKAAEVARNLHMRLSKEKADFLLFKVLRVDTAGCLLKCSGHGHCDPLTKRCICSHLWMEN LIQRYIWDGESNCEWSIFYVTVLAFTLIVLTGGFTWLCICCCKRQKRTKIRKKTKYTILDNMDEQERMEL RPKYGIKHRSTEHNSSLMVSESEFDSDQDTIFSREKMERGNPKVSMNGSIRNGASFSYCSKDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252328
ORF Size	1449 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252328.1, NP_001239257.1
RefSeq Size	5315 bp
RefSeq ORF	1452 bp
Locus ID	9856
Protein Families	Transmembrane
MW	54.5 kDa
Gene Summary	This gene encodes a transmembrane protein that contains a large extracellular domain with multiple polycystic kidney disease (PKD) domains. The encoded protein may play a role in the development of the cerebral cortex by regulating neuronal migration and cell adhesion. Single nucleotide polymorphisms in this gene are associated with dyslexia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

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