LONRF3 (NM_001289109) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RNF127
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238597 representing NM_001289109 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTTTAAGGCACATTTCAGAAAAGCCCAAGCCTTAGCCACCCTAGGCAAGGTGGAGGAGGCACTAA GGGAGTTTCTCTACTGTGTATCCCTTGATGGAAAGAACAAGAGAGCAAGATGTGAAGCCCAAAGACTTCT CCTTAGTTTCTTTTCACCATCAGTCCCGGGGGATTCTCAGGAACATTCTCCCGATATCCTGAAGCTGTTG GCACCTCACCCTAGATTAAAGGAAAACGTAGAGTCAATGACTACTGAAGTTACCAGCCATAATCTACCAA GGTTGTTACAGGACAATCTGGAGCTCCCACATTGTTCTAGTCAGGAGGAAGCAGCAGCCAGGGGAGATGG CAGCAGTCTGATGGACCCAGCTAAAGTGAAGGGGGATGGTCAGCAGCACCACATGAAAGACCAGGAAGAA GAGGAGGAGAAGTGGGATGCTACCTCTCCAAAAGCTGCTTCCAGCAAGACTGGAAAATGCCAGGAAAAGA AAAGGAAACATTGCCAGATTGAATCCCAAGAAGAAACGGGGATGCCTAATAAAGCCTCCAAGCAAGATCC TCCCACTGATCAGGGGGACAAACCTGCTCTCAGTTTACCACTTGCATCTTTCGACGCATCTGACCTTGAA TGCGCTCTATGTATGAGATTATTCTATGAGCCAGTCACAACACCTTGCGGGCATACTTTTTGCTTAAAAT GCCTAGAAAGATGCCTAGATCACAACGCAAAGTGTCCATTGTGCAAAGACGGTCTTTCACAGTGCTTGGC ATCAAGAAAATACAGCAAAAATGTAATAATGGAGGAGCTCATAGCTAAATTCCTTCCAGAAGAACTGAAG GAACGAAGGAAGCTTTATGAAGAGGAAATGGAAGAACTTTCTAACCTTAATAAGAATGTGCCTATTTTCG TGTGTACTATGGCCTATCCCACCGTTCCTTGTCCCCTGCACATCTTTGAGCCTTGTTACCGCCTGATGAT TCGTAGATGCATTGAGACAGGCACGAGACAGTTTGGCATGTGCCTTGGAGATCCTGTCAAAGGGTTTGCA GAATATGGCTGCATCCTAGAGATCAGAAATGTTCAATTCTTTGCTGATGGCCGCTCAGTGGTTGACAGCA TAGGCAAGAGGCGCTTCAGGGTGCTCCATCAGAGCCAGCGGGATGGCTACAACACAGCCGACATTGAATA CATTGAAGACCAAAAGGTTCAGGGAGAGGATTGTGCTGAGCTCATGGGATTACATAACTGTGTCTATCAG CAAGCATCATTGTGGTTTCATTCGCTCAAATTATCCCTAAAGAATCGGATACTCAATCACTTTGGTCCCA TGCCGGAGAAAGACGCCGATCCTCAGATGAACCCGAATGGCCCAGCCTGGTGCTGGTGGATGTTAGCAGT TCTTCCCTTGGAAAGCCGAGCTCAGCTCCCCTTCCTAGCAATGAGGTCCTTAAAGGACAGACTGAATGGT ATTCGACGAGTCCTGGCCTTCATATCCCGAAACCAAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238597 representing NM_001289109 Red=Cloning site Green=Tags(s) MGFKAHFRKAQALATLGKVEEALREFLYCVSLDGKNKRARCEAQRLLLSFFSPSVPGDSQEHSPDILKLL APHPRLKENVESMTTEVTSHNLPRLLQDNLELPHCSSQEEAAARGDGSSLMDPAKVKGDGQQHHMKDQEE EEEKWDATSPKAASSKTGKCQEKKRKHCQIESQEETGMPNKASKQDPPTDQGDKPALSLPLASFDASDLE CALCMRLFYEPVTTPCGHTFCLKCLERCLDHNAKCPLCKDGLSQCLASRKYSKNVIMEELIAKFLPEELK ERRKLYEEEMEELSNLNKNVPIFVCTMAYPTVPCPLHIFEPCYRLMIRRCIETGTRQFGMCLGDPVKGFA EYGCILEIRNVQFFADGRSVVDSIGKRRFRVLHQSQRDGYNTADIEYIEDQKVQGEDCAELMGLHNCVYQ QASLWFHSLKLSLKNRILNHFGPMPEKDADPQMNPNGPAWCWWMLAVLPLESRAQLPFLAMRSLKDRLNG IRRVLAFISRNQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289109
ORF Size	1509 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289109.1, NP_001276038.1
RefSeq Size	2898 bp
RefSeq ORF	1512 bp
Locus ID	79836
Protein Families	Druggable Genome, Protease
MW	57.6 kDa
Gene Summary	The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants have been suggested, but their full length natures are not clear. [provided by RefSeq, Jul 2008]

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