SPATA18 (NM_001297608) Human Tagged ORF Clone
CAT#: RC238613
- TrueORF®
SPATA18 (myc-DDK-tagged) - Human spermatogenesis associated 18 (SPATA18), transcript variant 2
ORF Plasmid: tGFP
"NM_001297608" in other vectors (2)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Mieap; SPETEX1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238613 representing NM_001297608
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAAACCTGAAAAGACTGGTCTCAAACGAAACTTTACGAACGTTGCAGGAAAAGCTAGACTTCT GGCTGAAGGAGTACAACACAAACACGTGTGATCAAAATCTAAACCATTGCCTTGAACTCATTGAGCAAGT TGCCAAGGTGCAGGGACAACTCTTTGGGATCCTCACAGCAGCAGCCCAAGAAGGAGGACGTAATGATGGT GTGGAAACAATCAAGTCACGCCTTTTGCCTTGGCTGGAGGCTTCCTTTACTGCTGCTTCCCTGGGAAAAT CTGTTGACAGCAAGGTCCCCTCTCTGCAGGACACGTTTGATAGGGAGAGACATAAAGATCCCAGTCCTCG GGATCGGGATATGCAACAGTTAGACTCTAATTTGAACTCAACCCGGAGTCAATGCAACCAGGTTCAAGAC GAGCTTAAATCTCTTCAAGCTCAGGAGGATGCCCGCCACAGAAACACAGATCAGAGGAGCTCAGAGAATA GGCGGTCAGAGCCTTGGAGCTTGGAGGAGCGGAAGCGTGAGCAGTGGAACTCACTCAAGCAGAATGCAGA CCAGCAGGACACAGAAGCCATGTCCGATTATAAGAAACAGCTCCGAAACCTGAAGGAGGAGATAGCTGTT CTGTCTGCTGAGAAAAGTGCACTCCAAGGAAGGTCCTCCAGGAGCCGGTCTCCCAGCCCTGCCCCTCGCA GCCGTAGCTGCAGCCGCAGCAGATCTGCCAGCCCCTCCACCGCTGTCAAGGTCAGGAGACCGTCCCCAAA CCGCTCCAAGCTGTCCAATGTGGCGCGCAAGGCTGCCCTCTTGTCCCGGTTCAGCGATTCCTATTCCCAG GCCCGCCTGGACGCGCAGTGCCTGCTGCGGCGCTGCATCGACAAGGCTGAGACCGTTCAGCGGATCATCT ACATCGCCACAGTGGAGGCATTCCATGTAGCAAAAATGGCATTCAGACACTTCAAGATCCATGTGAGAAA ATCGTTGACACCATCTTATGTGGGGTCGAATGACTTTGAGAATGCTGTCTTGGATTATGTCATTTGTCAT CTTGATCTATATGATTCTCAAAGCAGTGTCAATGATGTGATCCGAGCCATGAATGTCAATCCCAAGATTT CATTCCCTCCTGTCGTTGACTTTTGCCTTCTCAGTGACTTCATCCAGGAGATATGTTGCATTGCCTTTGC AATGCAGGCCTTAGAACCACCCCTAGATATTGCATATGGAGCAGATGGAGAAGTTTTTAATGATTGCAAA TACCGCCGCAGCTACGACTCGGATTTCACTGCTCCCTTAGTCCTCTATCACGTGTGGCCTGCTCTCATGG AGAATGACTGTGTCATTATGAAGGGAGAAGCTGTCACCAGGAGAGGGGCTTTTTGGAATTCGGTGCGATC TGTAAGTCGTTGTCGAAGCAGGAGTTTAAGTCCCATTTGCCCCCGTAGCCAAATTGGTTTAAACACGATG TCTCGAAGTCGGAGTCCTTCTCCAATAAGATGTGGATTGCCAAGATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238613 representing NM_001297608
Red=Cloning site Green=Tags(s) MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDG VETIKSRLLPWLEASFTAASLGKSVDSKVPSLQDTFDRERHKDPSPRDRDMQQLDSNLNSTRSQCNQVQD ELKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAV LSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQ ARLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIHVRKSLTPSYVGSNDFENAVLDYVICH LDLYDSQSSVNDVIRAMNVNPKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAYGADGEVFNDCK YRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVTRRGAFWNSVRSVSRCRSRSLSPICPRSQIGLNTM SRSRSPSPIRCGLPRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001297608 |
ORF Size | 1518 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297608.2 |
RefSeq Size | 4329 bp |
RefSeq ORF | 1521 bp |
Locus ID | 132671 |
UniProt ID | Q8TC71 |
MW | 57.9 kDa |
Gene Summary | This gene encodes a p53-inducible protein that is able to induce lysosome-like organelles within mitochondria that eliminate oxidized mitochondrial proteins, thereby contributing to mitochondrial quality control. Dysregulation of mitochondrial quality control is associated with cancer and degenerative diseases. The encoded protein mediates accumulation of the lysosome-like mitochondrial organelles through interaction with B cell lymphoma 2 interacting protein 3 and B cell lymphoma 2 interacting protein 3 like at the outer mitochondrial membrane, which allows translocation of lysosomal proteins to the mitochondrial matrix from the cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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