APPBP2 (NM_001282476) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	APP-BP2; HS.84084; PAT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238658 representing NM_001282476 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTACTACAAGACATTTGCTTCATCATTGTTTTCAGGCTTTGATGGATCATGGTGTTAAAGTTGCTT CAGTCTTGGCCTACTCATTCAGTAGGCGGTGCTCTTATATAGCAGAATCAGATGCTGCAGTAAAGGAAAA AGCCATTCAGGTTGGCTTTGTTTTAGGTGGCTTTCTTTCAGATGCAGGCTGGTACAGTGATGCTGAGAAA GTTTTTCTGTCCTGCCTTCAGTTGTGTACTCTACACGATGAGATGCTTCATTGGTTTCGTGCAGTAGAAT GTTGTGTGAGGTTGCTTCATGTGCGAAATGGAAACTGCAAATATCATTTGGGTGAAGAAACATTTAAATT AGCTCAGACATATATGGATAAACTATCAAAACATGGCCAGCAAGCAAATAAAGCTGCACTCTATGGAGAA CTGTGTGCACTCCTATTTGCAAAAAGTCACTATGATGAGGCATACAAATGGTGCATCGAGGCAATGAAAG AAATTACAGCAGGCTTACCAGTGAAAGTTGTGGTGGATGTCTTAAGACAAGCTTCTAAGGCTTGTGTAGT AAAACGTGAATTTAAGAAGGCAGAACAGTTAATTAAACATGCAGTGTATTTGGCACGGGATCATTTTGGA TCCAAACACCCAAAATATTCTGATACACTGCTAGATTATGGGTTCTACTTACTCAATGTAGATAATATCT GTCAGTCTGTTGCAATTTATCAGGCAGCCCTTGACATTAGACAGTCAGTGTTTGGTGGCAAAAATATCCA CGTAGCAACAGCTCATGAAGATTTGGCCTACTCTTCTTATGTCCACCAGTATAGCTCTGGGAAATTTGAC AATGCACTATTTCATGCAGAAAGAGCTATTGGTATCATTACCCACATCCTACCTGAAGATCATCTTCTTT TGGCTTCTTCAAAGAGGGTGAAAGCACTTATTTTAGAGGAGATTGCAATTGATTGTCATAATAAGGAAAC TGAACAGAGGCTGCTTCAAGAAGCTCATGATTTGCACCTGTCTTCACTCCAACTAGCTAAAAAAGCTTTT GGGGAATTTAATGTACAGACTGCAAAACACTATGGAAACCTTGGAAGACTTTATCAGTCAATGAGAAAAT TTAAGGAAGCTGAAGAAATGCACATCAAAGCAATTCAGATTAAAGAACAACTTCTTGGTCAAGAAGATTA TGAAGTAGCCCTTTCAGTGGGACATCTGGCTTCTTTATATAATTATGACATGAATCAGTATGAAAATGCT GAGAAACTTTATTTGCGATCTATAGCAATTGGGAAGAAACTTTTTGGTGAGGGCTACAGTGGACTAGAAT ATGATTATCGAGGTCTCATTAAACTTTACAACTCCATTGGAAATTACGAGAAAGTGTTTGAATATCACAA TGTTCTGTCTAACTGGAACCGGTTGCGAGATCGGCAATATTCAGTGACAGATGCTCTTGAAGATGTCAGC ACCAGCCCCCAGTCCACTGAAGAAGTGGTGCAGTCCTTCCTGATTTCTCAGAATGTCGAGGGACCGAGCT GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238658 representing NM_001282476 Red=Cloning site Green=Tags(s) MFTTRHLLHHCFQALMDHGVKVASVLAYSFSRRCSYIAESDAAVKEKAIQVGFVLGGFLSDAGWYSDAEK VFLSCLQLCTLHDEMLHWFRAVECCVRLLHVRNGNCKYHLGEETFKLAQTYMDKLSKHGQQANKAALYGE LCALLFAKSHYDEAYKWCIEAMKEITAGLPVKVVVDVLRQASKACVVKREFKKAEQLIKHAVYLARDHFG SKHPKYSDTLLDYGFYLLNVDNICQSVAIYQAALDIRQSVFGGKNIHVATAHEDLAYSSYVHQYSSGKFD NALFHAERAIGIITHILPEDHLLLASSKRVKALILEEIAIDCHNKETEQRLLQEAHDLHLSSLQLAKKAF GEFNVQTAKHYGNLGRLYQSMRKFKEAEEMHIKAIQIKEQLLGQEDYEVALSVGHLASLYNYDMNQYENA EKLYLRSIAIGKKLFGEGYSGLEYDYRGLIKLYNSIGNYEKVFEYHNVLSNWNRLRDRQYSVTDALEDVS TSPQSTEEVVQSFLISQNVEGPSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282476
ORF Size	1542 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282476.1, NP_001269405.1
RefSeq Size	6410 bp
RefSeq ORF	1545 bp
Locus ID	10513
UniProt ID	Q92624
Protein Families	Druggable Genome
MW	59 kDa
Gene Summary	The protein encoded by this gene interacts with microtubules and is functionally associated with beta-amyloid precursor protein transport and/or processing. The beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. The encoded protein may be involved in regulating cell death. This gene has been found to be highly expressed in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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