IGF2BP2 (NM_001291872) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IMP-2; IMP2; VICKZ2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238814 representing NM_001291872 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCATGTCCTGGACACTACCACGTTGATGGCTTTCTAAATCCAGGGAGCAGGAAAATTCAGATTC GAAACATCCCTCCTCACCTGCAGTGGGAGGTGTTGGATGGACTTTTGGCTCAATATGGGACAGTGGAGAA TGTGGAACAAGTTTTTGCCTTTTCCCTAGTCAACACAGACACAGAAACCGCCGTTGTCAACGTCACATAT GCAACAAGAGAAGAAGCAAAAATAGCCATGGAGAAGCTAAGCGGGCATCAGTTTGAGAACTACTCCTTCA AGATTTCCTACATCCCGGATGAAGAGGTGAGCTCCCCTTCGCCCCCTCAGCGAGCCCAGCGTGGGGACCA CTCTTCCCGGGAGCAAGGCCACGCCCCTGGGGGCACTTCTCAGGCCAGACAGATTGATTTCCCGCTGCGG ATCCTGGTCCCCACCCAGTTTGTTGGTGCCATCATCGGAAAGGAGGGCTTGACCATAAAGAACATCACTA AGCAGACCCAGTCCCGGGTAGATATCCATAGAAAAGAGAACTCTGGAGCTGCAGAGAAGCCTGTCACCAT CCATGCCACCCCAGAGGGGACTTCTGAAGCATGCCGCATGATTCTTGAAATCATGCAGAAAGAGGCAGAT GAGACCAAACTAGCCGAAGAGATTCCTCTGAAAATCTTGGCACACAATGGCTTGGTTGGAAGACTGATTG GAAAAGAAGGCAGAAATTTGAAGAAAATTGAACATGAAACAGGGACCAAGATAACAATCTCATCTTTGCA GGATTTGAGCATATACAACCCGGAAAGAACCATCACTGTGAAGGGCACAGTTGAGGCCTGTGCCAGTGCT GAGATAGAGATTATGAAGAAGCTGCGTGAGGCCTTTGAAAATGATATGCTGGCTGTTAACCAACAAGCCA ATCTGATCCCAGGGTTGAACCTCAGCGCACTTGGCATCTTTTCAACAGGACTGTCCGTGCTATCTCCACC AGCAGGGCCCCGCGGAGCTCCCCCCGCTGCCCCCTACCACCCCTTCACTACCCACTCCGGATACTTCTCC AGCCTGTACCCCCATCACCAGTTTGGCCCGTTCCCGCATCATCACTCTTATCCAGAGCAGGAGATTGTGA ATCTCTTCATCCCAACCCAGGCTGTGGGCGCCATCATCGGGAAGAAGGGGGCACACATCAAACAGCTGGC GAGATTCGCCGGAGCCTCTATCAAGATTGCCCCTGCGGAAGGCCCAGACGTCAGCGAAAGGATGGTCATC ATCACCGGGCCACCGGAAGCCCAGTTCAAGGCCCAGGGACGGATCTTTGGGAAACTGAAAGAGGAAAACT TCTTTAACCCCAAAGAAGAAGTGAAGCTGGAAGCGCATATCAGAGTGCCCTCTTCCACAGCTGGCCGGGT GATTGGCAAAGGTGGCAAGACCGTGAACGAACTGCAGAACTTAACCAGTGCAGAAGTCATCGTGCCTCGT GACCAAACGCCAGATGAAAATGAGGAAGTGATCGTCAGAATTATCGGGCACTTCTTTGCTAGCCAGACTG CACAGCGCAAGATCAGGGAAATTGTACAACAGGTGAAGCAGCAGGAGCAGAAATACCCTCAGGGAGTCGC CTCACAGCGCAGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238814 representing NM_001291872 Red=Cloning site Green=Tags(s) MFSCPGHYHVDGFLNPGSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVFAFSLVNTDTETAVVNVTY ATREEAKIAMEKLSGHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLR ILVPTQFVGAIIGKEGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEAD ETKLAEEIPLKILAHNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASA EIEIMKKLREAFENDMLAVNQQANLIPGLNLSALGIFSTGLSVLSPPAGPRGAPPAAPYHPFTTHSGYFS SLYPHHQFGPFPHHHSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVI ITGPPEAQFKAQGRIFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPR DQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291872
ORF Size	1626 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291872.3
RefSeq Size	3498 bp
RefSeq ORF	1629 bp
Locus ID	10644
UniProt ID	Q9Y6M1
MW	60.1 kDa
Gene Summary	This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016]

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