CEP70 (NM_001288966) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BITE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238874 representing NM_001288966 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCCGGTAGCCCCTAAACCCCAGGATTCCAGTCAACCATCAGACAGACTCATGACTGAAAAACAGC AGGAAGAAGCAGAATGGGAAAGCATAAATGTGCTATTGATGATGCATGGCTTAAAACCTTTGTCTCTAGT CAAAAGAACAGATCTCAAAGATCTCATCATTTTTGACAAACAGTCATCACAAAGGATGAGACAGAATTTG AAATTGTTGGTGGAAGAAACATCATGTCAACAGAACATGATACAGGAGCTTATAGAAACTAATCAACAGC TTAGAAATGAACTTCAGCTAGAGCAAAGCCGAGCAGCCAATCAAGAACAACGAGCTAATGACTTGGAACA AATTATGGAAAGTGTGAAATCCAAAATTGGTGAATTGGAGGATGAATCACTAAGTAGGGCTTGCCACCAA CAGAATAAAATAAAAGATCTTCAAAAGGAGCAGAAAACTTTACAGGTGAAGTGCCAGCATTATAAGAAAA AACGAACGGAGCAAGAAGAAACTATTGCTTCTTTGCAAATGGAAGTCTGTAGATTAAAAAAGGAGGAAGA AGATCGCATTGTCACTCAAAACAGAGTGTTTGCCTATCTGTGCAAAAGAGTTCCTCATACCGTCTTGGAT AGACAGTTGCTTTGTCTAATTGATTACTATGAATCTAAAATTAGAAAAATTCATACACAAAGGCAATATA AAGAAGATGAAAGTCAGTCAGAAGAAGAAAACGACTACAGAAATCTGGATGCCTCACCAACTTATAAAGG CCTTTTAATGTCATTACAGAATCAACTCAAGGAATCAAAATCTAAGATTGATGCACTTTCAAGTGAAAAG CTGAACCTCCAAAAAGATTTGGAAACCAGGCCTACGCAGCATGAATTAAGACTTTATAAACAGCAGGTGA AGAAGCTGGAAAAAGCCCTTAAGAAAAACGTCAAATTACAGGAGCTTATTAATCATAAGAAGGCTGAGGA CACAGAGAAGAAAGATGAGCCCAGCAAATATAATCAGCAACAGGCCCTAATTGACCAGAGATACTTTCAG GTGCTGTGTAGCATCAATTCAATTATCCACAATCCAAGAGCTCCAGTAATAATTTATAAACAGACCAAAG GGGGAGTCCAAAATTTTAATAAAGATCTTGTTCAAGATTGTGGATTTGAGCATCTTGTTCCTGTAATAGA AATGTGGGCAGATCAACTGACATCCTTAAAGGATTTGTATAAGTCCTTGAAAACACTATCTGCAGAACTG GTACCTTGGCTTAATTTGAAGAAGCAGGATGAAAATGAAGGTATCAAAGTTGAAGATTTGTTGTTTATAG TAGATACTATGCTGGAAGAAGTTGAAAATAAGGAAAAGGACAGCAATATGCCACACTTTCAAACTTTGCA AGCTATTGTTTCTCACTTCCAAAAGTTATTTGATGTGCCTTCTTTAAATGGAGTCTATCCCCGAATGAAT GAAGTTTATACTAGGCTTGGAGAAATGAACAATGCTGTGAGAAACCTCCAAGAACTCTTAGAATTAGATA GTTCATCCTCATTGTGTGTGCTAGTAAGCACTGTTGGAAAACTCTGTAGGCTGATTAATGAAGATGTGAA TGAGCAGGTTATGCAGGTATTAGGACCTGAAGACCTCCAGAGGTATTTGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238874 representing NM_001288966 Red=Cloning site Green=Tags(s) MFPVAPKPQDSSQPSDRLMTEKQQEEAEWESINVLLMMHGLKPLSLVKRTDLKDLIIFDKQSSQRMRQNL KLLVEETSCQQNMIQELIETNQQLRNELQLEQSRAANQEQRANDLEQIMESVKSKIGELEDESLSRACHQ QNKIKDLQKEQKTLQVKCQHYKKKRTEQEETIASLQMEVCRLKKEEEDRIVTQNRVFAYLCKRVPHTVLD RQLLCLIDYYESKIRKIHTQRQYKEDESQSEEENDYRNLDASPTYKGLLMSLQNQLKESKSKIDALSSEK LNLQKDLETRPTQHELRLYKQQVKKLEKALKKNVKLQELINHKKAEDTEKKDEPSKYNQQQALIDQRYFQ VLCSINSIIHNPRAPVIIYKQTKGGVQNFNKDLVQDCGFEHLVPVIEMWADQLTSLKDLYKSLKTLSAEL VPWLNLKKQDENEGIKVEDLLFIVDTMLEEVENKEKDSNMPHFQTLQAIVSHFQKLFDVPSLNGVYPRMN EVYTRLGEMNNAVRNLQELLELDSSSSLCVLVSTVGKLCRLINEDVNEQVMQVLGPEDLQRYLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288966
ORF Size	1662 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288966.1, NP_001275895.1
RefSeq Size	2187 bp
RefSeq ORF	1665 bp
Locus ID	80321
UniProt ID	Q8NHQ1
MW	65.3 kDa
Gene Summary	Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.[UniProtKB/Swiss-Prot Function]

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