Filensin (BFSP1) (NM_001278607) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CP94; CP115; CTRCT33; LIFL-H
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238877 representing NM_001278607 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTTCAAATTTCACAGAAGGAGCCTCCATGCTGGAGAAAGTATGAAAATGAGTGCGAATGTCAAC TCCTGCTAAAAGAAATGCTTGAACGGCTTAACAAGGAAGCTGATGAAGCCTTGCTGCATAACCTACGCCT TCAGCTGGAAGCCCAATTTCTGCAAGATGATATCAGTGCGGCAAAGGACAGGCACAAGAAGAATCTTCTG GAAGTTCAGACCTATATCAGCATCCTGCAGCAGATCATCCACACCACTCCTCCAGCATCCATTGTGACGA GTGGGATGAGGGAGGAGAAGCTCCTGACGGAGCGGGAGGTGGCCGCCCTGCGGAGTCAGCTGGAGGAGGG CCGGGAGGTGCTCTCCCACCTGCAGGCGCAGAGAGTGGAGCTGCAGGCACAGACAACAACTCTGGAACAA GCTATTAAAAGTGCCCATGAGTGTTATGACGATGAGATTCAGCTTTATAACGAGCAGATTGAGACACTGC GCAAGGAGATTGAGGAGACAGAGCGGGTCCTGGAGAAGTCTTCTTACGACTGCCGGCAGCTGGCGGTCGC CCAGCAAACCCTGAAGAATGAGCTGGACCGGTATCATCGTATCATCGAGATTGAAGGCAACAGGCTGACC TCTGCCTTCATTGAAACTCCCATTCCCCTGTTCACCCAGAGCCATGGAGTCTCTCTCAGCACTGGATCCG GTGGGAAAGATCTTACCAGAGCTCTGCAGGATATAACAGCAGCAAAACCAAGACAAAAAGCCCTCCCCAA GAATGTTCCAAGGAGAAAAGAGATTATAACAAAAGACAAAACCAACGGAGCTCTGGAAGATGCACCATTA AAAGGTTTGGAAGACACAAAGCTGGTACAGGTGGTACTTAAAGAGGAAAGTGAATCTAAGTTTGAATCAG AAAGTAAAGAAGTAAGTCCCCTGACACAAGAAGGGGCTCCAGAGGATGTGCCAGATGGAGGGCAGATAAG CAAAGGCTTTGGGAAACTATACAGGAAGGTCAAGGAGAAAGTGAGAAGCCCCAAAGAGCCTGAGACCCCC ACTGAGCTCTACACCAAAGAGCGGCACGTGCTGGTCACAGGGGATGCCAATTACGTGGACCCTAGATTCT ATGTCTCCTCCATCACAGCTAAAGGTGGGGTGGCTGTTTCTGTTGCGGAAGACTCTGTGCTTTATGACGG CCAGGTGGAGCCCTCTCCTGAGTCACCCAAGCCCCCTTTAGAGAATGGGCAGGTGGGTCTGCAGGAGAAA GAAGATGGACAACCAATTGACCAGCAGCCTATAGACAAGGAGATTGAGCCAGATGGTGCAGAGCTGGAAG GCCCTGAAGAGAAACGTGAGGGTGAGGAGCGGGACGAAGAGTCCAGGAGACCCTGTGCCATGGTCACACC CGGTGCAGAGGAACCATCTATACCTGAGCCTCCAAAGCCTGCGGCTGATCAGGATGGAGCTGAGGTGCTT GGGACTAGGAGCAGAAGCCTGCCAGAAAAAGGCCCTCCCAAGGCTTTGGCCTATAAGACAGTGGAAGTGG TGGAATCTATCGAGAAGATTTCCACGGAGAGCATTCAGACATATGAAGAAACCGCTGTGATCGTGGAGAC CATGATTGGAAAGACAAAGTCAGACAAGAAGAAATCAGGAGAGAAGAGCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238877 representing NM_001278607 Red=Cloning site Green=Tags(s) MDFQISQKEPPCWRKYENECECQLLLKEMLERLNKEADEALLHNLRLQLEAQFLQDDISAAKDRHKKNLL EVQTYISILQQIIHTTPPASIVTSGMREEKLLTEREVAALRSQLEEGREVLSHLQAQRVELQAQTTTLEQ AIKSAHECYDDEIQLYNEQIETLRKEIEETERVLEKSSYDCRQLAVAQQTLKNELDRYHRIIEIEGNRLT SAFIETPIPLFTQSHGVSLSTGSGGKDLTRALQDITAAKPRQKALPKNVPRRKEIITKDKTNGALEDAPL KGLEDTKLVQVVLKEESESKFESESKEVSPLTQEGAPEDVPDGGQISKGFGKLYRKVKEKVRSPKEPETP TELYTKERHVLVTGDANYVDPRFYVSSITAKGGVAVSVAEDSVLYDGQVEPSPESPKPPLENGQVGLQEK EDGQPIDQQPIDKEIEPDGAELEGPEEKREGEERDEESRRPCAMVTPGAEEPSIPEPPKPAADQDGAEVL GTRSRSLPEKGPPKALAYKTVEVVESIEKISTESIQTYEETAVIVETMIGKTKSDKKKSGEKSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278607
ORF Size	1662 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278607.2
RefSeq Size	2073 bp
RefSeq ORF	1665 bp
Locus ID	631
UniProt ID	Q12934
MW	62.5 kDa
Gene Summary	This gene encodes a lens-specific intermediate filament-like protein named filensin. The encoded protein is expressed in lens fiber cells after differentiation has begun. This protein functions as a component of the beaded filament which is a cytoskeletal structure found in lens fiber cells. Mutations in this gene are the cause of autosomal recessive cortical juvenile-onset cataract. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

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