Smek1 (PPP4R3A) (NM_001284281) Human Tagged ORF Clone
CAT#: RC239025
- TrueORF®
SMEK1 (myc-DDK-tagged) - Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1), transcript variant 4
ORF Plasmid: tGFP
"NM_001284281" in other vectors (2)
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CNY 5,890.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FLFL1; KIAA2010; MSTP033; PP4R3; PP4R3A; SMEK1; smk-1; smk1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239025 representing NM_001284281
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGCA CCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGA CGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACAACAGGAAGATGAAAAA TTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGGTTA ACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGAGATGCTTTTTTCAAGAC TTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATGATACACAGGTGCGAAGT GCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAGTTTGTCATGCAGG AGGCACAACAGAATGATGATGTAAGTAAGAAGTTAACAGAGCAAAAAATAACCAGCAAGGATATTTTGCT CATCAACCTCATTATAGAACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTTATG GGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAAAACAGAAAAGACTGAAT TTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGA CAAACCTAGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATTGTTAACATTTTGT GTGGAGCACCATACCTACCACATAAAGAACTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTC TTATGGCCTCGAAGCATGCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAA AGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAACCAGTAGTGAAAGCATTTCTCAAC AATGGATCCCGCTACAATCTGATGAACTCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATA TAAAATCATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGATGTAGATTATGTACAGAC ATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGAGAAAGGCAAGATAATCCCAAACTTGACAGTATG CGTTCCATTTTGAGGAATCACAGATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGT TTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCTCCATCTGACAAAACTAAAAATGA TGATGATATTATGGATCCAATAAGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAA GTGCTTCTGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTGTCCAGTGGAACGA AGACTAACCTCACCAGCCAGTCATCTACAACAAATCTGCCTGGTTCTCCGGGATCACCTGGATCCCCAGG ATCTCCAGGCTCTCCTGGATCCGTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGAGGC CTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGAGGATGAAGATAAGGAAGATACGT TACCATTGTCAAAGAAAGCAAAATTTGATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239025 representing NM_001284281
Red=Cloning site Green=Tags(s) MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQEDEK FLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRS AATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLM GLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFC VEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLN NGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSM RSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKE VLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGG LVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001284281 |
ORF Size | 1782 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001284281.1, NP_001271210.1 |
RefSeq Size | 3896 bp |
RefSeq ORF | 1785 bp |
Locus ID | 55671 |
UniProt ID | Q6IN85 |
MW | 68.3 kDa |
Gene Summary | Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |