Znf206 (ZSCAN10) (NM_001282416) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZFP206; ZNF206
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239187 representing NM_001282416 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCCAGTATCAGGAGGACATGGGGCCACGGGCGTCCCTGAGCCGGCTCCGGGAGCTCTGCGGCCAC TGGCTGCGGCCGGCTCTGCACACCAAGAAACAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTGAGTG TGCTGCCTCCGCACCTCCTGGGCCGCCTGCAGGGGCAGCCGCTCAGGGATGGGGAGGAGGTGGTGCTGCT GCTCGAGGGCATCCACCGGGAGCCCAGCCACGCGGGGCCGCTGGTCCCCAGGGCCCCTCACCATGGCCAG AGGAGAGTTCCCGAGATCAGGAGCTGGCGGCTGTGCTGGAGTGCCTGACCTTTGAGGATGTGCCAGAGAA TAAGGCGTGGCCTGCACACCCCCTGGGATTCGGAAGCAGAACCCCAGACAAGGAGGAATTTAAACAAGAA GAGCCCAAAGGGGCTGCCTGGCCCACTCCCATCTTAGCAGAGTCCCAGGCAGATAGTCCTGGGGTGCCGG GAGAGCCTTGCGCCCAGTCGCTCGGACGGGGCGCTGCGGCTAGCGGCCCTGGTGAAGATGGGTCCCTTCT TGGCAGCAGTGAAATTTTGGAGGTCAAAGTGGCTGAGGGCGTCCCCGAGCCCAATCCGGAGTTGCAGTTC ATCTGCGCGGACTGCGGGGTGAGCTTCCCGCAGCTGTCTCGCCTGAAGGCGCACCAGCTGCGCTCGCACC CGGCTGGGCGCTCCTTCCTGTGCCTTTGCTGCGGGAAGAGCTTCGGCCGCAGCTCCATTCTCAAGCTGCA CATGCGCACTCACACGGACGAGCGGCCGCACGCCTGCCACCTGTGCGGCCACCGCTTCCGCCAGAGCTCG CACCTGAGCAAGCACCTGCTGACCCACTCCTCCGAACCCGCCTTCCTGTGCGCAGAGTGCGGCCGCGGCT TCCAGCGCCGCGCCAGCCTTGTGCAGCACCTGCTGGCGCACGCCCAGGACCAGAAGCCGCCCTGCGCTCC TGAGAGTAAGGCCGAAGCGCCGCCACTGACCGATGTCCTGTGCTCCCACTGCGGCCAGAGCTTCCAGCGC CGCTCCAGCCTCAAGCGCCACCTGCGGATCCACGCCAGGGACAAGGACCGCCGGTCCTCCGAAGGCTCCG GCAGCCGCCGCCGGGACTCCGACCGGAGGCCCTTCGTGTGCAGCGACTGCGGCAAGGCCTTCCGGCGCAG CGAGCACCTGGTGGCCCACCGGAGGGTGCACACGGGCGAGCGGCCCTTCTCCTGCCAGGCTTGCGGCCGC AGCTTCACGCAGAGCTCGCAGCTGGTCAGCCACCAACGGGTGCACACGGGCGAGAAGCCCTACGCCTGTC CGCAGTGCGGGAAGCGCTTTGTGCGCCGGGCCAGCCTTGCCCGCCACCTGCTGACCCACGGTGGCCCTCG GCCCCACCACTGCACCCAGTGCGGGAAGAGTTTCGGCCAGACCCAGGATCTGGCCCGCCACCAGCGCAGC CACACGGGCGAGAAGCCCTGCCGCTGCAGCGAGTGCGGTGAGGGCTTCAGCCAGAGCGCCCACCTGGCGC GCCACCAGCGCATCCACACAGGGGAGAAGCCCCACGCCTGCGACACCTGCGGCCACCGTTTCCGCAATAG CTCCAACCTGGCCCGCCATCGCCGCAGCCACACGGGCGAGCGGCCCTACAGCTGTCAGACGTGCGGTCGC AGCTTCCGGCGCAACGCGCATCTGCGGCGGCACCTGGCTACCCATGCGGAGCCCGGGCAGGAGCAGGCCG AGCCCCCGCAGGAGTGCGTGGAGTGCGGGAAGAGCTTCAGCCGCAGCTGCAATCTGCTGCGACACCTGCT GGTGCACACGGGCGCCAGGCCCTACTCCTGCACGCAGTGTGGCCGCAGCTTCAGCCGCAACTCCCACCTG CTGCGCCACCTGCGCACCCACGCCCGCGAGACGCTGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239187 representing NM_001282416 Red=Cloning site Green=Tags(s) MLPVSGGHGATGVPEPAPGALRPLAAAGSAHQETDPGAAGAGAVPECAASAPPGPPAGAAAQGWGGGGAA ARGHPPGAQPRGAAGPQGPSPWPEESSRDQELAAVLECLTFEDVPENKAWPAHPLGFGSRTPDKEEFKQE EPKGAAWPTPILAESQADSPGVPGEPCAQSLGRGAAASGPGEDGSLLGSSEILEVKVAEGVPEPNPELQF ICADCGVSFPQLSRLKAHQLRSHPAGRSFLCLCCGKSFGRSSILKLHMRTHTDERPHACHLCGHRFRQSS HLSKHLLTHSSEPAFLCAECGRGFQRRASLVQHLLAHAQDQKPPCAPESKAEAPPLTDVLCSHCGQSFQR RSSLKRHLRIHARDKDRRSSEGSGSRRRDSDRRPFVCSDCGKAFRRSEHLVAHRRVHTGERPFSCQACGR SFTQSSQLVSHQRVHTGEKPYACPQCGKRFVRRASLARHLLTHGGPRPHHCTQCGKSFGQTQDLARHQRS HTGEKPCRCSECGEGFSQSAHLARHQRIHTGEKPHACDTCGHRFRNSSNLARHRRSHTGERPYSCQTCGR SFRRNAHLRRHLATHAEPGQEQAEPPQECVECGKSFSRSCNLLRHLLVHTGARPYSCTQCGRSFSRNSHL LRHLRTHARETLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282416
ORF Size	1929 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282416.2
RefSeq Size	2476 bp
RefSeq ORF	1932 bp
Locus ID	84891
UniProt ID	Q96SZ4
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
MW	70.1 kDa
Gene Summary	Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency. Can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.[UniProtKB/Swiss-Prot Function]

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