PCDHB6 (NM_001303145) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PCDH-BETA6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239235 representing NM_001303145 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTGAAAATATCAGAAATTACTATGCCAGGAAAGATATTTCCTTTGAAAATGGCACACGATTTAG ACACCGGCAGCAACGGCCTTCAGAGGTACACAATCAGCTCCAACCCTCACTTCCACGTTCTCACCCGCAA TCGCAGCGAAGGCAGGAAGTTCCCGGAGCTGGTGCTAGACAAACCGTTGGACCGCGAGGAGCAGCCCCAA CTCAGGCTAACGCTGATCGCGCTGGATGGCGGGTCTCCGCCCCGGTCAGGGACCTCCGAGATTCAGATCC AGGTTTTGGACATCAATGACAACGTCCCCGAGTTTGCTCAGGAGCTCTATGAAGCACAAGTCCCTGAGAA CAACCCCCTCGGCTCTCTGGTTATTACCGTCTCAGCCAGAGATTTAGATGCAGGATCGTTTGGGAAGGTA TCTTACGCCCTGTTTCAAGTCGATGACGTCAACCAACCCTTCGAAATAAACGCAATCACAGGAGAAATTC GGCTGAGAAAGGCTTTGGATTTTGAGGAAATTCAGTCTTATGACGTGGATGTTGAGGCTACAGATGGTGG AGGCCTATCAGGAAAATGCTCTTTAGTCGTCAGGGTCCTGGACGTGAATGACAATGCCCCTGAACTCACC ATGTCGTTCTTCATCAGCCTCATCCCAGAAAACTTACCAGAGATCACAGTGGCAGTTTTCAGTGTTTCAG ATGCAGACTCTGGACATAACCAACAGGTTATTTGTTCAATAGAGAACAATCTCCCCTTTCTACTAAGACC TTCCGTGGAGAATTTCTACACCCTGGTAACAGAAGGCGCGCTGGACAGAGAGAGCAGAGCCGAGTACAAC ATCACTATCACGGTCACTGATTTGGGGACACCAAGGCTGAAAACCCAGCAGAGCATAACTGTGCAGGTCT CCGACGTCAATGACAACGCCCCCGCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAG CCCCGCCCTGCACATCGGCAGCGTCAGCGCCACAGACAGAGACTCAGGCATCAACGCCCAGGTCACCTAC TCGCTGCTGCCGCCCCAGGACCCGCACCTGCCCCTCTCTTCCCTGGTCTCCATCAACGCGGACAACGGCC ACCTGTTTGCCCTCAGGTCGCTGGACTACGAGGCCCTGCAGTCTTTCGAGTTCCGCGTGGGCGCCACAGA CCGCGGCTCCCCGGCGTTGAGCAGCGAGGCGCTGGTGCGCTTGCTGGTGCTGGACGCCAACGACAACTCG CCCTTCGTGTTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACCGAGCTGGTGCCCCGGGCGGCCGAGC CGGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAACGCCTGGCTGTCGTACCA GCTGCTCAAGGCCACGGAGCTCGGTCTGTTCGGCGTGTGGGCGCACAATGGCGAGGTGCGCACCGCCAGG CTGCTGAGCGAGCGAGACGCAGCCAAGCACAGGCTGGTGGTGCTTGTCAAGGACAATGGCGAGCCTCCGC GCTCGGCCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCCTCTCCCTGA GGCGGCCCCGGCCCAAGCCCAGGCCGACTCTCTCACCGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCG TCGCTCTTCCTCTTTTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCTCGG TGGGTCGCTACTCGGTGCCCGAGGGTCCCTTTCCAGGGCATCTGGTGGATGTGAGCGGCACCGGGACCCT ATCCCAGAGCTACCAGTACAAGGTGTGTCTGACGGGAGGCTCAGAAACAAATGAGTTCAAGTTCCTGAAG CCGATTATGCCCAACTTCCCTCCTCAGGGCACTGAGAGAGAAATGGAAGAAACCCCCACCTCTCGGAATA GCTTCCCGTTCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239235 representing NM_001303145 Red=Cloning site Green=Tags(s) MLLKISEITMPGKIFPLKMAHDLDTGSNGLQRYTISSNPHFHVLTRNRSEGRKFPELVLDKPLDREEQPQ LRLTLIALDGGSPPRSGTSEIQIQVLDINDNVPEFAQELYEAQVPENNPLGSLVITVSARDLDAGSFGKV SYALFQVDDVNQPFEINAITGEIRLRKALDFEEIQSYDVDVEATDGGGLSGKCSLVVRVLDVNDNAPELT MSFFISLIPENLPEITVAVFSVSDADSGHNQQVICSIENNLPFLLRPSVENFYTLVTEGALDRESRAEYN ITITVTDLGTPRLKTQQSITVQVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGINAQVTY SLLPPQDPHLPLSSLVSINADNGHLFALRSLDYEALQSFEFRVGATDRGSPALSSEALVRLLVLDANDNS PFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATELGLFGVWAHNGEVRTAR LLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVS SLFLFSVLLFVAVRLCRRSRAASVGRYSVPEGPFPGHLVDVSGTGTLSQSYQYKVCLTGGSETNEFKFLK PIMPNFPPQGTEREMEETPTSRNSFPFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303145
ORF Size	1974 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303145.2
RefSeq Size	3061 bp
RefSeq ORF	1977 bp
Locus ID	56130
UniProt ID	Q9Y5E3
Protein Families	Transmembrane
MW	72.2 kDa
Gene Summary	This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. Unlike the alpha and gamma clusters, the transcripts from these genes do not share common 3' exons. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell neural connections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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