MED15 (NM_001293236) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1; TNRC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239293 representing NM_001293236 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTTTCCGGGCAAGAGACCGACTGGCGGAGCACCGCCTTCCGGCAGAAGCTGGTCAGTCAAATCG AGGATGCCATGAGGAAAGCTGGTGTGGCACACAGTAAATCCAGCAAGGATATGGAGAGCCATGTTTTCCT GAAGGCCAAGACCCGGGACGAATACCTTTCTCTCGTGGCCAGGCTCATTATCCATTTTCGAGACATTCAT AACAAGAAATCTCAAGCTTCCGTCAGTGCCCAGCTGCAGCTCCAGCAGGTGGCGCTGCAGCAGCAGCAGC AACAGCAGCAGTTCCAGCAGCAGCAGCAGGCGGCGCTACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCA GTTCCAGGCTCAGCAGAGTGCCATGCAGCAGCAGTTCCAAGCAGTAGTGCAGCAGCAGCAGCAGCTCCAG CAGCAGCAGCAGCAGCAGCAGCATCTAATTAAATTGCATCATCAAAATCAGCAACAGATACAGCAGCAGC AACAGCAGCTGCAGCGAATAGCACAGCTGCAGCTCCAACAACAGCAACAGCAGCAGCAGCAGCAGCAGCA GCAGCAGCAGCAGGCTTTGCAGGCCCAGCCACCAATTCAGCAGCCACCGATGCAGCAGCCACAGCCTCCG CCCTCCCAGGCTCTGCCCCAGCAGCTGCAGCAGATGCATCACACACAGCACCACCAGCCGCCACCACAGC CCCAGCAGCCTCCAGTTGCTCAGAACCAACCATCACAACTCCCGCCACAGTCGCAGACCCAGCCTTTGGT GTCACAGGCGCAAGCTCTCCCTGGACAAATGTTGTATACCCAACCACCACTGAAATTTGTCCGAGCTCCG ATGGTGGTGCAGCAGCCCCCAGTGCAGCCCCAGGTGCAGCAGCAGCAGACAGCAGTACAGACAGCTCAGG CTGCCCAGATGGTGGCTCCCGGAGTCCAGGTCAGCCAGAGCAGCCTCCCCATGCTGTCCTCGCCGTCACC GGGCCAGCAGGTGCAGACCCCGCAGTCGATGCCCCCTCCCCCCCAGCCGTCCCCGCAGCCCGGCCAGCCC AGCTCACAGCCCAACTCCAACGTCAGCTCTGGCCCTGCCCCATCTCCCAGTAGCTTCCTGCCCAGCCCCT CACCGCAGCCCTCCCAGAGCCCAGTGACGGCGCGGACCCCACAGAACTTCAGTGTCCCCTCACCTGGACC TTTAAACACACCTGTGAACCCCAGCTCTGTCATGAGCCCAGCTGGCTCCAGCCAGGCTGAGGAGCAGCAG TACCTGGACAAGCTGAAGCAGCTGTCGAAGTACATCGAGCCCCTGCGCCGCATGATCAACAAGATCGACA AGAACGAAGACAGAAAAAAGGACCTGAGTAAGATGAAGAGCCTTCTGGACATTCTGACAGACCCCTCGAA GCGGTGTCCCCTGAAGACCTTGCAAAAGTGTGAGATCGCCCTGGAGAAACTCAAGAATGACATGGCGGTG CCCACTCCCCCACCGCCCCCGGTGCCACCGACCAAACAGCAGTACCTATGCCAGCCGCTCCTGGATGCCG TCCTGGCCAACATCCGCTCACCTGTCTTCAACCATTCCCTGTACCGCACATTCGTTCCAGCCATGACCGC CATTCACGGCCCACCCATCACGGCCCCAGTGGTGTGCACCCGGAAGCGCAGGCTTGAGGATGATGAGCGG CAGAGCATCCCCAGTGTGCTCCAGGGTGAGGTGGCCAGGCTGGACCCCAAGTTCCTGGTAAACCTGGACC CTTCTCACTGCAGCAACAATGGCACTGTCCACCTGATCTGCAAGCTGGATGACAAGGACCTCCCAAGTGT GCCACCACTGGAGCTCAGTGTGCCCGCTGACTATCCTGCCCAAAGCCCGCTGTGGATAGACCGGCAGTGG CAGTACGACGCCAACCCCTTCCTCCAGTCGGTGCACCGCTGCATGACCTCCAGGCTGCTGCAGCTCCCGG ACAAGCACTCGGTCACCGCCTTGCTCAACACCTGGGCCCAGAGCGTCCACCAGGCCTGCCTCTCAGCCGC C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239293 representing NM_001293236 Red=Cloning site Green=Tags(s) MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIH NKKSQASVSAQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQ QQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQPP PSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRAP MVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQP SSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQ YLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAV PTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDER QSIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQW QYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSVHQACLSAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293236
ORF Size	2031 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293236.2
RefSeq Size	3118 bp
RefSeq ORF	2034 bp
Locus ID	51586
UniProt ID	Q96RN5
Protein Families	Druggable Genome, Transcription Factors
MW	76.4 kDa
Gene Summary	The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.