AGAP8 (AGAP4) (NM_001276343) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AGAP-4; AGAP-8; AGAP8; CTGLF1; CTGLF5; MRIP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239304 representing NM_001276343 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACATACTGACCTGTCGTGTGCACCCTAGCGTCAGCCTCGAGTTTGACCAGCAGCAGGGGTCGG TGTGTCCCTCTGAATCTGAGATCTATGAGGCAGGAGCTGGGGACAGGATGGCAGGAGCGCCCATGGCTGC TGCTGTACAGCCTGCTGAGGTGACTGTTGAAGTTGGTGAGGACCTCCACATGCACCACGTTCGTGACCGG GAGATGCCTGAAGCTTTGGAGTTTAACCTTTCTGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACT CTCAAACAGATGCTTTGGAGTTTAACCCTTCTGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACTC TCAAACAGATGTTGTAGAAATAAGAAGAAGCAACTGTACAAACCATGTATCTACTGTGCGTTTCAGTCAA CAATACAGCTTGTGTTCGACAATATTCCTTGATGACAGCACAGCCATCCAGCATTATCTTACAATGACAA TAATATCTGTGACCTTGGAGATACCTCATCATATCACACAAAGAGATGCAGATAGATCTTTGAGCATACC TGATGAACAGTTACACTCATTTGCGGTTTCCACCGTGCACATTATGAAGAAAAGAAATGGAGGTGGGAGT TTAAATAACTATTCCTCCTCCATTCCATCGACTCCCAGCACCAGCCAGGAGGACCCTCAGTTCAGTGTTC CTCCCACTGCCAACACACCCACCCCCGTTTGCAAGCGGTCCATGCGCTGGTCCAACCTGTTTACATCTGA GAAAGGGAGTGACCCAGACAAAGAGAGGAAAGCCCCGGAGAATCATGCTGACACCATCGGGAGCGGCAGA GCCATCCCCATTAAACAGGGCATGCTCTTAAAGCGAAGTGGGAAATGGCTGAAGACATGGAAAAAGAAAT ACGTCACCCTGTGTTCCAATGGCGTGCTCACCTATTATTCAAGCTTAGGTGATTATATGAAGAATATTCA TAAAAAAGAGATTGACCTTCAGACATCTACCATCAAAGTCCCAGGAAAGTGGCCATCCCTAGCCACATCG GCCTGCACACCCATCTCCACCTCTAAAAGCAATGGCCTATCCAAGGACATGGACACCGGGCTGGGTGACT CCATATGCTTCAGCCCCAGTATCTCCAGCACCACCAGCCCCAAGCTCAACCCGCCCCCCTCTCCTCATGC CAATAAAAAGAAACACCTAAAGAAGAAAAGCACCAACAACTTTATGATTGTGTCTGCCACTGGCCAAACG TGGCACTTTGAAGCCACGACGTATGAGGAGCGGGATGCCTGGGTCCAAGCCATCCAGAGCCAGATCCTGG CCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTCCCAGCTGACCAGCCAGAGCAAGGCCATGGCCCT GCAGTCGATCCAAAACATGCGTGGGAACGCCCACTGTGTGGACTGTGAGACCCAGAATCCTAAGTGGGCC AGTTTGAACTTGGGAGTCCTCATGTGTATTGAATGCTCAGGAATCCACCGCAGTCTTGGCACCCGCCTTT CCCGTGTGCGATCTCTGGAGCTGGATGACTGGCCAGTTGAGCTCAGGAAGGTTATGTCATCTATTGGCAA TGACCTAGCCAACAGCATCTGGGAAGGGAGCAGCCAGGGGCAGACAAAACCCTCAGAAAAGTCCACGAGG GAAGAGAAGGAACGGTGGATCCGTTCCAAATATGAGGAGAAGCTCTTTCTGGCCCCACTACCCTGCACTG AGCTGTCCCTGGGCCAGCAGCTGCTGCGGGCCACCGCTGATGAGGACCTGCAGACAGCCATCCTGCTGCT GGCACATGGCTCCCGTGAGGAGGTGAACGAGACCTGTGGGGAGGGAGACGGCTGCACGGCGCTCCATCTG GCCTGCCGCAAGGGGAATGTGGTCCTGGCGCAGCTCCTGATCTGGTACGGGGTGGACGTCATGGCCCGAG ATGCCCACGGGAACACAGCGCTGACCTACGCCCGGCAGGCCTCCAGCCAGGAGTGCATCAACGTGCTTCT GCAGTACGGCTGCCCCGACAAGTGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239304 representing NM_001276343 Red=Cloning site Green=Tags(s) MGNILTCRVHPSVSLEFDQQQGSVCPSESEIYEAGAGDRMAGAPMAAAVQPAEVTVEVGEDLHMHHVRDR EMPEALEFNLSANPEASTIFQRNSQTDALEFNPSANPEASTIFQRNSQTDVVEIRRSNCTNHVSTVRFSQ QYSLCSTIFLDDSTAIQHYLTMTIISVTLEIPHHITQRDADRSLSIPDEQLHSFAVSTVHIMKKRNGGGS LNNYSSSIPSTPSTSQEDPQFSVPPTANTPTPVCKRSMRWSNLFTSEKGSDPDKERKAPENHADTIGSGR AIPIKQGMLLKRSGKWLKTWKKKYVTLCSNGVLTYYSSLGDYMKNIHKKEIDLQTSTIKVPGKWPSLATS ACTPISTSKSNGLSKDMDTGLGDSICFSPSISSTTSPKLNPPPSPHANKKKHLKKKSTNNFMIVSATGQT WHFEATTYEERDAWVQAIQSQILASLQSCESSKSKSQLTSQSKAMALQSIQNMRGNAHCVDCETQNPKWA SLNLGVLMCIECSGIHRSLGTRLSRVRSLELDDWPVELRKVMSSIGNDLANSIWEGSSQGQTKPSEKSTR EEKERWIRSKYEEKLFLAPLPCTELSLGQQLLRATADEDLQTAILLLAHGSREEVNETCGEGDGCTALHL ACRKGNVVLAQLLIWYGVDVMARDAHGNTALTYARQASSQECINVLLQYGCPDKCV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276343
ORF Size	2058 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276343.2, NP_001263272.2
RefSeq Size	2520 bp
RefSeq ORF	2061 bp
Locus ID	119016
UniProt ID	Q96P64
MW	76.1 kDa
Gene Summary	Putative GTPase-activating protein.[UniProtKB/Swiss-Prot Function]

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