USP49 (NM_001286554) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239311 representing NM_001286554 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGACCACTCCATCCTGAACCCTCAGAAGT GGTGCTGCTTAGAGTGTGCCACCACCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGG CCGCTATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCTAGCCATGGAAGTCCGG GATCTCTACGTGTTCTGTTACCTGTGCAAGGACTACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGC TGCTAAGAAGCTCCCTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCGGACGCT GCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCGCTCCTCAGGGACAGCCGCAGATGCTC ACGGCTCTGTGGTACCGGCGTCAGCGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCC GGGGCCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGAAGGAGGAGGCGCGGAG GCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAGGAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGG CTGCTCCTGCACACGCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCTCACGCA GAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGGCCTGCGCAA CCTGGGCAACACCTGCTACATGAACTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGT TTCCTCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTT CTGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCG GGAGGGCTTCTGCTGGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCG AGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCGGGAAGTGGG CCCTAGTGTCGCCCTTCGCCATGCTCCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCA ACAGGACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACC ACACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCA TATTTCATGGGCAGCTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCATTGAGCC CTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTCCCTTTGAAT CAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCT ACGCTTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAG AAAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGC CGTAATCATCGAGAGAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCT GCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGTCATGCATCA CGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCTACAACACAGAGGGAGGTTTTTGGGTCCAC TGCAATGACTCAAAGCTGAATGTATGCAGTGTCGAGGAAGTGTGCAAAACCCAGGCCTACATCCTTTTTT ACACTCAAAGAACAGTGCAGGGCAATGCAAGAATCTCAGAAACCCATCTCCAAGCTCAGGTGCAGTCCAG CAACAATGATGAAGGCAGACCACAGACATTTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239311 representing NM_001286554 Red=Cloning site Green=Tags(s) MDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVR DLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQML TALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSAR LLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFREC FLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEP SSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGT TRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLN QTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSG RNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVH CNDSKLNVCSVEEVCKTQAYILFYTQRTVQGNARISETHLQAQVQSSNNDEGRPQTFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286554
ORF Size	2064 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286554.2
RefSeq Size	8822 bp
RefSeq ORF	2067 bp
Locus ID	25862
UniProt ID	Q70CQ1
Protein Families	Protease, Transcription Factors
MW	79.6 kDa
Gene Summary	Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.[UniProtKB/Swiss-Prot Function]

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