CDT2 (DTL) (NM_001286230) Human Tagged ORF Clone
CAT#: RC239313
- TrueORF®
DTL (myc-DDK-tagged) - Human denticleless E3 ubiquitin protein ligase homolog (Drosophila) (DTL), transcript variant 3
ORF Plasmid: tGFP
"NM_001286230" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CDT2; DCAF2; L2DTL; RAMP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239313 representing NM_001286230
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTCAATTCGGTGCTCCGCCAGCCCCAGCTTGGCGTCCTGAGAAATGCTCCCAATATGGAACATG TACTAGCAGTTGCCAATGAAGAAGGCTTTGTTCGATTGTATAACACAGAATCACAAAGTTTCAGAAAGAA GTGCTTCAAAGAATGGATGGCTCACTGGAATGCCGTCTTTGACCTGGCCTGGGTTCCTGGTGAACTTAAA CTTGTTACAGCAGCAGGTGATCAAACAGCCAAATTTTGGGACGTAAAAGCTGGTGAGCTGATTGGAACAT GCAAAGGTCATCAATGCAGCCTCAAGTCAGTTGCCTTTTCTAAGTTTGAGAAAGCTGTATTCTGTACGGG TGGAAGAGATGGCAACATTATGGTCTGGGATACCAGGTGCAACAAAAAAGATGGGTTTTATAGGCAAGTG AATCAAATCAGTGGAGCTCACAATACCTCAGACAAGCAAACCCCTTCAAAACCCAAGAAGAAACAGAATT CAAAAGGACTTGCTCCTTCTGTGGATTTCCAGCAAAGTGTTACTGTGGTCCTCTTTCAAGACGAGAATAC CTTAGTCTCAGCAGGAGCTGTGGATGGGATAATCAAAGTATGGGATTTACGTAAGAATTATACTGCTTAT CGACAAGAACCCATAGCATCCAAGTCTTTCCTGTACCCAGGTAGCAGCACTCGAAAACTTGGATATTCAA GTCTGATTTTGGATTCCACTGGCTCTACTTTATTTGCTAATTGCACAGACGATAACATCTACATGTTTAA TATGACTGGGTTGAAGACTTCTCCAGTGGCTATTTTCAATGGACACCAGAACTCTACCTTTTATGTAAAA TCCAGCCTTAGTCCAGATGACCAGTTTTTAGTCAGTGGCTCAAGTGATGAAGCTGCCTACATATGGAAGG TCTCCACACCCTGGCAACCTCCTACTGTGCTCCTGGGTCATTCTCAAGAGGTCACGTCTGTGTGCTGGTG TCCATCTGACTTCACAAAGATTGCTACCTGTTCTGATGACAATACACTAAAAATCTGGCGCTTGAATAGA GGCTTAGAGGAGAAACCAGGAGGTGATAAACTTTCCACGGTGGGTTGGGCCTCTCAGAAGAAAAAAGAGT CAAGACCTGGCCTAGTAACAGTAACGAGTAGCCAGAGTACTCCTGCCAAAGCCCCCAGGGTAAAGTGCAA TCCATCCAATTCTTCCCCGTCATCCGCAGCTTGTGCCCCAAGCTGTGCTGGAGACCTCCCTCTTCCTTCA AATACTCCTACGTTCTCTATTAAAACCTCTCCTGCCAAGGCCCGGTCTCCCATCAACAGAAGAGGCTCTG TCTCCTCCGTCTCTCCCAAGCCACCTTCATCTTTCAAGATGTCGATTAGAAACTGGGTGACCCGAACACC TTCCTCATCACCACCCATCACTCCACCTGCTTCGGAGACCAAGATCATGTCTCCGAGAAAAGCCCTTATT CCTGTGAGCCAGAAGTCATCCCAAGCAGAGGCTTGCTCTGAGTCTAGAAATAGAGTAAAGAGGAGGCTAG ACTCAAGCTGTCTGGAGAGTGTGAAACAAAAGTGTGTGAAGAGTTGTAACTGTGTGACTGAGCTTGATGG CCAAGTTGAAAATCTTCATTTGGATCTGTGCTGCCTTGCTGGTAACCAGGAAGACCTTAGTAAGGACTCT CTAGGTCCTACCAAATCAAGCAAAATTGAAGGAGCTGGTACCAGTATCTCAGAGCCTCCGTCTCCTATCA GTCCGTATGCTTCAGAAAGCTGTGGAACGCTACCTCTTCCTTTGAGACCTTGTGGAGAAGGGTCTGAAAT GGTAGGCAAAGAGAATAGTTCCCCAGAGAATAAAAACTGGTTGTTGGCCATGGCAGCCAAACGGAAGGCT GAGAATCCATCTCCACGAAGTCCGTCATCCCAGACACCCAATTCCAGGAGACAGAGCGGAAAGACATTGC CAAGCCCGGTCACCATCACGCCCAGCTCCATGAGGAAAATCTGCACATACTTCCATAGAAAGTCCCAGGA GGACTTCTGTGGTCCTGAACACTCAACAGAATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239313 representing NM_001286230
Red=Cloning site Green=Tags(s) MLFNSVLRQPQLGVLRNAPNMEHVLAVANEEGFVRLYNTESQSFRKKCFKEWMAHWNAVFDLAWVPGELK LVTAAGDQTAKFWDVKAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQV NQISGAHNTSDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKVWDLRKNYTAY RQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAIFNGHQNSTFYVK SSLSPDDQFLVSGSSDEAAYIWKVSTPWQPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKIWRLNR GLEEKPGGDKLSTVGWASQKKKESRPGLVTVTSSQSTPAKAPRVKCNPSNSSPSSAACAPSCAGDLPLPS NTPTFSIKTSPAKARSPINRRGSVSSVSPKPPSSFKMSIRNWVTRTPSSSPPITPPASETKIMSPRKALI PVSQKSSQAEACSESRNRVKRRLDSSCLESVKQKCVKSCNCVTELDGQVENLHLDLCCLAGNQEDLSKDS LGPTKSSKIEGAGTSISEPPSPISPYASESCGTLPLPLRPCGEGSEMVGKENSSPENKNWLLAMAAKRKA ENPSPRSPSSQTPNSRRQSGKTLPSPVTITPSSMRKICTYFHRKSQEDFCGPEHSTEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286230 |
ORF Size | 2064 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286230.1, NP_001273159.1 |
RefSeq Size | 4485 bp |
RefSeq ORF | 2067 bp |
Locus ID | 51514 |
Protein Families | Druggable Genome |
MW | 75.5 kDa |
Gene Summary | Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613, PubMed:27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207).[UniProtKB/Swiss-Prot Function] |
Documents
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SDS |