PRMT7 (NM_001290018) Human Tagged ORF Clone
CAT#: RC239320
- TrueORF®
PRMT7 (myc-DDK-tagged) - Human protein arginine methyltransferase 7 (PRMT7), transcript variant 3
ORF Plasmid: tGFP
"NM_001290018" in other vectors (2)
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CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SBIDDS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239320 representing NM_001290018
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATCTTCTGCAGTCGGGCCAATCCGACCACGGGGTCTGTGGAGTGGCTGGAGGAGGATGAACACT ATGATTACCACCAGGAGATTGCAAGGTCATCTTATGCAGATATGCTACATGACAAAGACAGAAATGTAAA ATACTACCAAGGTATCCGGGCTGCCGTGAGCAGGGTGAAGGACAGAGGACAGAAGGCCTTGGTTCTCGAC ATTGGCACTGGCACGGGACTCTTGTCAATGATGGCGGTCACAGCAGGTGCCGACTTCTGCTATGCCATCG AGGTTTTCAAGCCTATGGCTGATGCTGCTGTGAAGATTGTGGAGAAAAATGGCTTTAGTGATAAGATTAA GGTTATCAACAAGCATTCCACCGAGGTGACTGTAGGTCCAGAGGGTGACATGCCATGCCGTGCCAACATC CTGGTCACAGAGTTGTTTGACACAGAGCTGATCGGGGAGGGGGCGCTGCCCTCCTATGAGCACGCACACA GGCATCTCGTGGAGGAAAATTGTGAGGCCGTGCCCCACAGAGCCACCGTCTATGCACAGCTGGTGGAGTC CGGGAGGATGTGGTCGTGGAACAAGCTATTTCCCATCCACGTGCAGACCAGCCTCGGAGAGCAGGTCATC GTCCCTCCCGTTGACGTGGAGAGCTGCCCTGGCGCACCCTCTGTCTGTGACATTCAGCTGAACCAGGTGT CACCAGCCGACTTTACAGTCCTCAGCGATGTGCTGCCCATGTTCAGCATAGACTTCAGCAAGCAAGTCAG TAGCTCAGCAGCCTGCCATAGCAGGCGGTTTGAACCTCTGACATCTGGCCGAGCTCAGGTGGTTCTCTCG TGGTGGGACATTGAAATGGACCCTGAGGGGAAGATCAAGTGCACCATGGCCCCCTTCTGGGCACACTCAG ACCCAGAGGAGATGCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTCCTGCCACAAGAGGAGCCTGT GGTGCAGGGCTCAGCGCTCTATCTGGTAGCCCACCACGATGACTACTGCGTATGGTACAGCCTGCAGAGG ACCAGCCCTGAAAAGAATGAGAGAGTCCGCCAGATGCGCCCCGTGTGTGACTGCCAGGCTCACCTGCTCT GGAACCGGCCTCGGTTTGGAGAGATCAATGACCAGGACAGAACTGATCGATACGTCCAGGCTCTGAGGAC CGTGCTGAAGCCAGACAGCGTGTGCCTGTGTGTCAGCGATGGCAGCCTGCTCTCCGTGCTGGCCCATCAC CTGGGGGTGGAGCAGGTGTTTACAGTCGAGAGTTCAGCAGCTTCTCACAAACTGTTGAGAAAAATCTTCA AGGCTAACCACTTGGAAGATAAAATTAACATCATAGAGAAACGGCCGGAATTATTAACAAATGAGGACCT ACAGGGCAGAAAGGTCTCTCTCCTCCTGGGCGAGCCGTTCTTCACTACCAGCCTGCTGCCGTGGCACAAC CTCTACTTCTGGTACGTGCGGACCGCTGTGGACCAGCACCTGGGGCCAGGTGCCATGGTGATGCCCCAGG CAGCCTCGCTGCACGCTGTGGTTGTGGAGTTCAGGGACCTGTGGCGGATCCGGAGCCCCTGTGGTGACTG CGAAGGCTTCGACGTGCACATCATGGACGACATGATTAAGCGTGCCCTGGACTTCAGGGAGAGCAGGGAA GCTGAGCCCCACCCGCTGTGGGAGTACCCATGCCGCAGCCTCTCCGAGCCCTGGCAGATCCTGACCTTTG ACTTCCAGCAGCCGGTGCCCCTGCAGCCCCTGTGTGCCGAGGGCACCGTGGAGCTCAGAAGGCCCGGGCA GAGCCACGCAGCGGTGCTATGGATGGAGTACCACCTGACCCCGGAGTGCACGCTCAGCACTGGCCTCCTG GAGCCTGCAGACCCCGAGGGGGGCTGCTGCTGGAACCCCCACTGCAAGCAGGCCGTCTACTTCTTCAGCC CTGCCCCAGATCCCAGAGCACTGCTGGGTGGCCCACGGACTGTCAGCTATGCAGTGGAGTTTCACCCCGA CACAGGCGACATCATCATGGAGTTCAGGCATGCAGATACCCCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239320 representing NM_001290018
Red=Cloning site Green=Tags(s) MKIFCSRANPTTGSVEWLEEDEHYDYHQEIARSSYADMLHDKDRNVKYYQGIRAAVSRVKDRGQKALVLD IGTGTGLLSMMAVTAGADFCYAIEVFKPMADAAVKIVEKNGFSDKIKVINKHSTEVTVGPEGDMPCRANI LVTELFDTELIGEGALPSYEHAHRHLVEENCEAVPHRATVYAQLVESGRMWSWNKLFPIHVQTSLGEQVI VPPVDVESCPGAPSVCDIQLNQVSPADFTVLSDVLPMFSIDFSKQVSSSAACHSRRFEPLTSGRAQVVLS WWDIEMDPEGKIKCTMAPFWAHSDPEEMQWRDHWMQCVYFLPQEEPVVQGSALYLVAHHDDYCVWYSLQR TSPEKNERVRQMRPVCDCQAHLLWNRPRFGEINDQDRTDRYVQALRTVLKPDSVCLCVSDGSLLSVLAHH LGVEQVFTVESSAASHKLLRKIFKANHLEDKINIIEKRPELLTNEDLQGRKVSLLLGEPFFTTSLLPWHN LYFWYVRTAVDQHLGPGAMVMPQAASLHAVVVEFRDLWRIRSPCGDCEGFDVHIMDDMIKRALDFRESRE AEPHPLWEYPCRSLSEPWQILTFDFQQPVPLQPLCAEGTVELRRPGQSHAAVLWMEYHLTPECTLSTGLL EPADPEGGCCWNPHCKQAVYFFSPAPDPRALLGGPRTVSYAVEFHPDTGDIIMEFRHADTPD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001290018 |
ORF Size | 2076 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290018.1, NP_001276947.1 |
RefSeq Size | 2984 bp |
RefSeq ORF | 2079 bp |
Locus ID | 54496 |
UniProt ID | Q9NVM4 |
Protein Families | Druggable Genome |
MW | 78.9 kDa |
Gene Summary | This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017] |
Documents
Product Manuals |
FAQs |
SDS |