Axotrophin (MARCH7) (NM_001282805) Human Tagged ORF Clone
CAT#: RC239360
- TrueORF®
MARCH7 (myc-DDK-tagged) - Human membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant 1
ORF Plasmid: tGFP
"NM_001282805" in other vectors (2)
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CNY 5,160.00
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AXO; AXOT; MARCH-VII; MARCH7; RNF177 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239360 representing NM_001282805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTAAACCTTCAAGGATTCCAAGAAGAATTTCTGTTCAACCTTCCAGCTCCTTAAGTGCTAGGA TGATGTCTGGAAGCAGAGGAAGTAGTTTAAATGATACCTATCACTCAAGAGACTCTTCATTTAGATTGGA TTCTGAATATCAGTCTACATCAGCATCAGCATCTGCGTCACCATTTCAATCTGCATGGTATAGTGAATCT GAGATAACTCAGGGAGCACGCTCAAGATCGCAGAACCAGCAACGGGATCATGATTCAAAAAGACCTAAAC TTTCCTGTACAAACTGTACTACCTCAGCTGGGAGAAATGTTGGAAATGGTTTAAACACATTATCAGATTC ATCTTGGAGGCATAGTCAAGTTCCTAGATCTTCATCAATGGTACTTGGATCATTTGGAACAGACTTAATG AGAGAGAGGAGAGATTTGGAGAGAAGAACAGATTCCTCTATTAGTAATCTTATGGATTATAGTCACCGAA GTGGTGATTTCACAACTTCATCATATGTTCAAGACAGAGTTCCTTCATATTCACAAGGAGCAAGACCAAA AGAAAACTCAATGAGCACTTTACAGTTGAATACATCATCCACAAACCACCAATTGCCTTCTGAACATCAG ACCATACTAAGTTCTAGGGACTCCAGAAATTCTTTAAGATCAAATTTTTCTTCAAGAGAATCAGAATCTT CCCGAAGCAATACGCAGCCTGGATTTTCTTACAGTTCAAGTAGAGATGAAGCCCCAATCATAAGCAATTC AGAAAGGGTTGTTTCATCTCAAAGACCATTTCAAGAATCTTCTGACAATGAAGGTAGGCGGACAACGAGG AGATTGCTGTCACGCATAGCTTCTAGCATGTCATCTACTTTTTTTTCACGAAGATCTAGTCAGGATTCCT TGAATACAAGATCATTGAATTCTGAAAATTCTTACGTTTCTCCAAGAATCTTGACAGCTTCACAGTCCCG TAGTAATGTACCATCAGCTTCTGAAGTTCCCGATAATAGGGCATCTGAAGCTTCTCAGGGATTTCGATTT CTTAGGCGAAGATGGGGTTTGTCATCTCTTAGCCACAATCATAGCTCTGAGTCAGATTCAGAAAATTTTA ACCAAGAATCTGAAGGTAGAAATACAGGACCATGGTTATCTTCCTCACTTAGAAATAGATGCACACCTTT GTTCTCTAGAAGGAGGCGAGAGGGAAGAGATGAATCTTCAAGGATACCTACCTCTGATACATCATCTAGA TCTCATATTTTTAGAAGAGAATCAAATGAAGTGGTTCACCTTGAAGCACAGAATGATCCTCTTGGAGCTG CTGCCAACAGACCACAAGCATCTGCAGCATCAAGCAGTGCCACAACAGGTGGCTCTACATCAGATTCGGC TCAAGGTGGAAGAAATACAGGAATATCAGGGATTCTTCCTGGTTCCTTATTCCGGTTTGCAGTCCCTCCA GCACTTGGGAGTAATTTGACCGACAATGTCATGATCACAGTAGATATTATTCCTTCAGGTTGGAATTCAG CTGATGGTAAAAGTGATAAAACTAAAAGTGCGCCTTCAAGAGATCCAGAAAGATTGCAGAAAATAAAAGA GAGCCTCCTTTTAGAGGACTCAGAAGAAGAAGAAGGTGACTTATGTAGAATTTGTCAAATGGCAGCTGCA TCATCATCTAATTTGCTGATAGAGCCATGCAAGTGCACAGGAAGTTTGCAGTATGTCCACCAAGACTGTA TGAAAAAGTGGTTACAGGCCAAAATTAACTCTGGTTCTTCATTAGAAGCTGTAACCACCTGTGAACTATG TAAAGAGAAGTTGGAGCTTAACCTGGAGGATTTTGATATTCATGAACTACATAGAGCTCATGCAAATGAA CAAGCTGAGTATGAGTTTATCAGCTCTGGTCTCTACCTAGTGGTGTTATTGCACTTGTGCGAACAAAGCT TTTCTGATATGATGGGAAATACAAATGAACCAAGCACACGTGTCCGATTTATTAACCTTGCAAGAACTCT TCAGGCACATATGGAAGATCTCGAAACTTCAGAGGATGATTCCGAAGAAGACGGAGACCATAACAGGACA TTTGATATTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239360 representing NM_001282805
Red=Cloning site Green=Tags(s) MESKPSRIPRRISVQPSSSLSARMMSGSRGSSLNDTYHSRDSSFRLDSEYQSTSASASASPFQSAWYSES EITQGARSRSQNQQRDHDSKRPKLSCTNCTTSAGRNVGNGLNTLSDSSWRHSQVPRSSSMVLGSFGTDLM RERRDLERRTDSSISNLMDYSHRSGDFTTSSYVQDRVPSYSQGARPKENSMSTLQLNTSSTNHQLPSEHQ TILSSRDSRNSLRSNFSSRESESSRSNTQPGFSYSSSRDEAPIISNSERVVSSQRPFQESSDNEGRRTTR RLLSRIASSMSSTFFSRRSSQDSLNTRSLNSENSYVSPRILTASQSRSNVPSASEVPDNRASEASQGFRF LRRRWGLSSLSHNHSSESDSENFNQESEGRNTGPWLSSSLRNRCTPLFSRRRREGRDESSRIPTSDTSSR SHIFRRESNEVVHLEAQNDPLGAAANRPQASAASSSATTGGSTSDSAQGGRNTGISGILPGSLFRFAVPP ALGSNLTDNVMITVDIIPSGWNSADGKSDKTKSAPSRDPERLQKIKESLLLEDSEEEEGDLCRICQMAAA SSSNLLIEPCKCTGSLQYVHQDCMKKWLQAKINSGSSLEAVTTCELCKEKLELNLEDFDIHELHRAHANE QAEYEFISSGLYLVVLLHLCEQSFSDMMGNTNEPSTRVRFINLARTLQAHMEDLETSEDDSEEDGDHNRT FDIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282805 |
ORF Size | 2112 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282805.2 |
RefSeq Size | 3664 bp |
RefSeq ORF | 2115 bp |
Locus ID | 64844 |
UniProt ID | Q9H992 |
MW | 78.1 kDa |
Gene Summary | MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG239360 | MARCH7 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant 1 |
CNY 6,180.00 |
|
SC337254 | MARCH7 (untagged) - Human membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant 1 |
CNY 6,750.00 |
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