IRAG1 (NM_001206880) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IRAG; JAW1L; MRVI1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239371 representing NM_001206880 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAAAAGCTCCCCAGAGTGAAGAGAGGCTGGCCAGAGGAGGAAAGGAGAATAACTCAGTTTTAGCCT GTGGAGCCCAGGCCTCTTGGAGCATCTTTGGGGCTGACGCAGCGGAGGTTCCGGGCACACGTGGCCACTC CCAGCAGGAGGCTGCCATGCCCCACATTCCCGAGGACGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAG AGCCCTGCCGGCCAAGGTCCTCCTGCCGCAGGAGTATCTTGCAGTCCAACTCCCACGATTGTCCTGACTG GGGATGCCACTTCACCAGAAGGAGAAACCGACAAAAACCTGGCCAACAGAGTTCACAGTCCCCACAAGAG GCTTTCTCACCGACACTTGAAGGTGTCCACTGCCTCCCTGACATCTGTGGACCCCGCGGGGCACATCATT GACCTGGTGAATGACCAGCTGCCAGACATCAGCATCTCAGAGGAGGACAAGAAGAAAAACCTGGCGCTGC TGGAAGAAGCCAAGTTGGTGAGTGAGCGATTCCTGACCCGCCGTGGGAGGAAGTCCAGGAGCAGCCCCGG AGACTCCCCATCAGCTGTTTCCCCGAACCTCAGCCCCAGCGCTTCTCCTACATCCTCTCGGAGCAACTCA CTTACAGTCCCCACCCCGCCAGGTTTGGATGTGTGCAGTGGCCCGCCATCCCCTCTGCCTGGAGCACCAC CACAGAAGGGGGATGAGGCCGACGTCTCTTCACCTCACCCTGGCGAGCCTGAGCACATCCTGATGAGAAA TCAGAACTTAGTGGGGCTCAAGCTTCCAGACCTTAGTGAAGCAGCTGAGCAGGAAAAAGGGCTTCCTTCT GAACTCTCCCCAGCTATTGAGGAAGAAGAGTCAAAGAGTGGCTTAGATGTCATGCCTAATATTTCTGATG TGCTGCTGCGCAAACTGCGGGTCCACAGGAGTCTCCCTGGAAGTGCCCCTCCACTCACTGAAAAGGAAGT TGAGAACGTGTTTGTGCAACTGTCCTTGGCCTTTAGAAATGACAGCTACACTCTGGAATCTAGAATTAAC CAGGCTGAAAGGGAACGCAACCTGACAGAGGAGAACACTGAGAAAGAACTGGAAAACTTCAAAGCTTCCA TTACGTCCTCAGCTTCACTCTGGCACCACTGTGAGCACCGGGAAACCTACCAGAAGTTGCTGGAGGACAT CGCTGTCCTGCACCGCCTGGCTGCCCGCCTCTCCAGCCGAGCTGAGGTGGTAGGCGCCGTCCGCCAGGAA AAGCGCATGTCGAAAGCAACGGAAGTGATGATGCAGTATGTGGAGAATCTAAAGAGGACGTATGAGAAGG ACCATGCGGAGCTCATGGAGTTTAAAAAGCTTGCAAATCAGAATTCAAGCCGCAGCTGTGGCCCCTCTGA AGATGGGGTCCCTCGCACGGCACGGTCCATGTCCCTCACGCTGGGAAAGAATATGCCTCGCCGGAGGGTC AGCGTTGCTGTGGTTCCTAAGTTTAATGCCCTGAATCTGCCTGGCCAAACTCCCAGCTCATCATCCATTC CCTCCTTACCAGCCTTGTCGGAATCACCCAATGGGAAAGGCAGCCTACCTGTCACTTCAGCACTGCCTGC ACTTTTGGAAAATGGAAAGACAAATGGGGACCCAGATTGTGAAGCCTCTGCTCCTGCGCTGACCCTGAGC TGCCTGGAGGAGCTTAGTCAGGAGACCAAGGCCAGGATGGAGGAAGAAGCCTACAGCAAGGGATTCCAAG AAGGTCTAAAGAAGACCAAAGAACTTCAAGACCTGAAGGAGGAGGAGGAAGAACAGAAGAGTGAGAGTCC TGAGGAACCTGAAGAGGTAGAAGAAACTGAGGAAGAGGAAAAGGGCCCAAGAAGCAGCAAACTTGAAGAA TTGGTCCATTTCTTACAAGTCATGTATCCCAAACTGTGTCAGCACTGGCAAGTGATCTGGATGATGGCTG CAGTGATGCTGGTCTTGACTGTTGTGCTGGGGCTCTACAATTCCTATAACTCTTGTGCAGAGCAGGCTGA TGGGCCCCTTGGAAGATCCACTTGCTCGGCAGCCCAGAGGGACTCCTGGTGGAGCTCAGGACTCCAGCAT GAGCAGCCTACAGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239371 representing NM_001206880 Red=Cloning site Green=Tags(s) MVKAPQSEERLARGGKENNSVLACGAQASWSIFGADAAEVPGTRGHSQQEAAMPHIPEDEEPPGEPQAAQ SPAGQGPPAAGVSCSPTPTIVLTGDATSPEGETDKNLANRVHSPHKRLSHRHLKVSTASLTSVDPAGHII DLVNDQLPDISISEEDKKKNLALLEEAKLVSERFLTRRGRKSRSSPGDSPSAVSPNLSPSASPTSSRSNS LTVPTPPGLDVCSGPPSPLPGAPPQKGDEADVSSPHPGEPEHILMRNQNLVGLKLPDLSEAAEQEKGLPS ELSPAIEEEESKSGLDVMPNISDVLLRKLRVHRSLPGSAPPLTEKEVENVFVQLSLAFRNDSYTLESRIN QAERERNLTEENTEKELENFKASITSSASLWHHCEHRETYQKLLEDIAVLHRLAARLSSRAEVVGAVRQE KRMSKATEVMMQYVENLKRTYEKDHAELMEFKKLANQNSSRSCGPSEDGVPRTARSMSLTLGKNMPRRRV SVAVVPKFNALNLPGQTPSSSSIPSLPALSESPNGKGSLPVTSALPALLENGKTNGDPDCEASAPALTLS CLEELSQETKARMEEEAYSKGFQEGLKKTKELQDLKEEEEEQKSESPEEPEEVEETEEEEKGPRSSKLEE LVHFLQVMYPKLCQHWQVIWMMAAVMLVLTVVLGLYNSYNSCAEQADGPLGRSTCSAAQRDSWWSSGLQH EQPTEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001206880
ORF Size	2118 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206880.2
RefSeq Size	5758 bp
RefSeq ORF	2121 bp
Locus ID	10335
UniProt ID	Q9Y6F6
Protein Families	Transmembrane
Protein Pathways	Vascular smooth muscle contraction
MW	77.3 kDa
Gene Summary	This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011]

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