CCDC108 (CFAP65) (NM_001278296) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CCDC108; SPGF40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239433 representing NM_001278296 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTCACCCAGGCTCCAAGCTCCGTCGTGAGGTCCAGGAACAGCAGGAACCACACCGTGAACTCTG GTGGATCCTGCCTGAGTGCCAGCACAGTGGCCATCCCTGCCATCAACGACAGCAGTGCAGCCATGAGTGC CTGCAGCACCATCAGCGCCCAGCCCGCAAGCTCCATGGACACTCAGATGCACTCCCCAAAGAAGCAGGAG AGAGTGAACAAGAGGGTCATCTGGGGCATTGAGGTGGCTGAGGAGCTGCATTGGAAAGGCTGGGAGCTAG GAAAGGAGACCACAAGGAATCTGGTTCTGAAAAATCGATCCTTGAAACTCCAGAAGATGAAGTACAGGCC CCCCAAGACCAAGTTCTTCTTCACGGTCATCCCTCAGCCCATCTTCCTGAGCCCAGGCATAACCCTCACG CTCCCCATCGTCTTCCGGCCTCTGGAGGCGAAGGAGTACATGGACCAGCTGTGGTTTGAGAAAGCGGAGG GGATGTTCTGTGTCGGCCTACGGGCCACCCTGCCCTGCCACAGGCTGATCTGCCGCCCACCATCCCTGCA GCTGCCCATGTGTGCTGTGGGAGATACGACTGAGGCCTTTTTCTGCCTGGATAATGTGGGGGACCTGCCC ACCTTCTTCACCTGGGAGTTCTCCAGCCCATTCCAGATGCTGCCCGCCACGGGGCTCCTGGAGCCAGGCC AGGCCTCTCAGATCAAGGTGACCTTTCAGCCCCTTACAGCCGTCATCTACGAGGTGCAGGCCACGTGCTG GTACGGGGCGGGCAGCCGGCAGAGGAGCAGCATCCAGCTGCAGGCTGTGGCCAAGTGCGCCCAGCTGCTG GTGAGCATAAAGCACAAGTGCCCGGAGGACCAGGATGCCGAGGGCTTCCAGAAGCTGTTGTACTTTGGCT CTGTTGCTGTGGGCTGCACCTCGGAGAGGCAGATCAGGCTACACAACCCGTCGGCGGTAAATGCCCCCTT CAGGATTGAAATTTCCCCGGATGAACTGGCCGAAGACCAGGCCTTCTCATGCCCCACGGCCCATGGCATC GTGCTTCCGGGAGAGAAGAAATGTGTGTCGGTGTTCTTCCACCCCAAGACTCTGGACACCAGAACTGTGG ACTACTGCTCCATCATGCCTTCTGGCTGTGCCTCCAAGACCCTGCTTAAAGTCGTTGGTTTCTGTAGAGG CCCTGCTGTGTCCCTGCAGCACTACTGTGTCAACTTCAGCTGGGTCAACCTTGGGGAGCGCTCCGAGCAG CCCCTGTGGATTGAGAACCAATCGGACTGCACGGCCCACTTCCAGTTTGCCATCGACTGCTTGGAGAGTG TCTTTACCATCAGGCCTGCCTTTGGGACGCTGGTGGGCAAGGCCCGTATGACCCTGCACTGTGCCTTCCA GCCCACTCACCCCATCATCTGCTTTCGGCGTGTGGCCTGTCTCATCCACCACCAGGACCCACTGTTCCTG GACCTGATGGGGACCTGCCACTCGGACAGCACCAAGCCAGCCATCCTGAAGCCTCAGCACCTCACCTGGT ACCGCACACACCTGGCCCGGGGCCTGACGCTCTACCCCCCTGACATCCTGGATGCCATGCTGAAGGAGAA GAAGCTGGCACAGGACCAGAACGGGGCTCTCATGATTCCCATCCAGGATCTGGAGGACATGCCGGCCCCG CAGTACCCTTATATCCCCCCCATGACCGAGTTCTTCTTCGACGGCACCAGCGACATAACCATCTTCCCCC CGCCCATCAGTGTAGAGCCTGTCGAGGTAGACTTCGGTGCCTGCCCAGGGCCTGAGGCCCCCAACCCTGT ACCCCTGTGCCTGATGAACCACACCAAGGGCAAGATCATGGTGGTCTGGACGCGAAGGTCTGACTGCCCC TTCTGGGTGACTCCAGAGAGCTGCGACGTGCCCCCACTCAAGTCCATGGCCATGCGCCTGCACTTCCAGC CGCCTCACCCCAACTGCCTTTACACGGTGGAGCTCGAAGCCTTCGCCATCTATAAGGTGTGTGCACGCAA TGAGAGGGAGGAATGCGGGGTCTCTGCTAGGAGCCTGAGTGGCTTGGTGGGGTGGCAGGAAGTGACCGAG GGCAGCTTCAGGCTCCATCCTCTGCGTGCCAGGCTTTCTCTTGGCTGGACAGTGACCCCTATGAGTTTGT CTCCTCCAAAGCTCCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239433 representing NM_001278296 Red=Cloning site Green=Tags(s) MMLTQAPSSVVRSRNSRNHTVNSGGSCLSASTVAIPAINDSSAAMSACSTISAQPASSMDTQMHSPKKQE RVNKRVIWGIEVAEELHWKGWELGKETTRNLVLKNRSLKLQKMKYRPPKTKFFFTVIPQPIFLSPGITLT LPIVFRPLEAKEYMDQLWFEKAEGMFCVGLRATLPCHRLICRPPSLQLPMCAVGDTTEAFFCLDNVGDLP TFFTWEFSSPFQMLPATGLLEPGQASQIKVTFQPLTAVIYEVQATCWYGAGSRQRSSIQLQAVAKCAQLL VSIKHKCPEDQDAEGFQKLLYFGSVAVGCTSERQIRLHNPSAVNAPFRIEISPDELAEDQAFSCPTAHGI VLPGEKKCVSVFFHPKTLDTRTVDYCSIMPSGCASKTLLKVVGFCRGPAVSLQHYCVNFSWVNLGERSEQ PLWIENQSDCTAHFQFAIDCLESVFTIRPAFGTLVGKARMTLHCAFQPTHPIICFRRVACLIHHQDPLFL DLMGTCHSDSTKPAILKPQHLTWYRTHLARGLTLYPPDILDAMLKEKKLAQDQNGALMIPIQDLEDMPAP QYPYIPPMTEFFFDGTSDITIFPPPISVEPVEVDFGACPGPEAPNPVPLCLMNHTKGKIMVVWTRRSDCP FWVTPESCDVPPLKSMAMRLHFQPPHPNCLYTVELEAFAIYKVCARNEREECGVSARSLSGLVGWQEVTE GSFRLHPLRARLSLGWTVTPMSLSPPKLLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278296
ORF Size	2190 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278296.2
RefSeq Size	3287 bp
RefSeq ORF	2193 bp
Locus ID	255101
Protein Families	Transmembrane
MW	81.8 kDa
Gene Summary	The protein encoded by this gene has putative coiled-coil domains and may be a transmembrane protein. The chicken ortholog of this gene is involved in the Rose-comb mutation, which is a large chromosome inversion, resulting in altered comb morphology and defects in sperm motility. [provided by RefSeq, Aug 2016]

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