ZNF227 (NM_001289167) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239466 representing NM_001289167 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGAACTTCAAGAACCTGGTTGCAGTGGGGCATCTTCCCTTCCAACCAGATATGGTATCCCAAT TGGAAGCAGAAGAAAAGCTTTGGATGATGGAAACAGAAACCCAAAGAAGCAGCAAGCATCAAAATAAGAT GGAAACACTCCAAAAATTTGCATTAAAATACCTTTCAAATCAAGAGCTGTCCTGCTGGCAAATCTGGAAA CAGGTTGCAAGTGAATTAACCAGGTGTCTTCAGGGGAAGAGTTCCCAGTTATTACAAGGTGACTCTATTC AGGTTTCTGAAAATGAGAACAATATAATGAACCCTAAAGGAGATAGCTCTATTTATATTGAAAATCAAGA GTTTCCATTTTGGAGAACCCAGCATTCTTGCGGGAATACATATCTGAGTGAGTCACAGATTCAGAGTAGA GGTAAGCAAATTGATGTGAAAAATAACCTGCAAATACATGAAGACTTCATGAAGAAATCACCATTTCATG AGCATATTAAAACTGACACAGAACCAAAACCCTGCAAAGGTAATGAATATGGCAAAATCATTAGTGATGG CTCCAATCAGAAATTACCCTTAGGAGAGAAACCCCATCCATGTGGTGAGTGTGGAAGGGGCTTCAGTTAT AGCCCAAGGCTTCCCCTTCATCCGAATGTTCATACAGGAGAAAAATGCTTCAGTCAAAGCTCACATCTGC GAACTCATCAGAGAATTCACCCAGGAGAGAAACTCAATAGATGTCATGAATCTGGTGATTGCTTCAATAA GAGCTCTTTTCATTCTTATCAATCTAATCATACAGGAGAGAAGTCTTATAGATGCGACAGTTGCGGCAAG GGATTCAGTAGCAGCACGGGTCTTATCATTCATTACAGAACTCATACTGGAGAGAAACCCTATAAATGCG AGGAATGTGGTAAATGCTTTAGTCAAAGTTCAAATTTTCAGTGCCATCAGAGAGTCCACACTGAAGAAAA ACCATACAAATGCGAAGAGTGTGGTAAGGGCTTCGGTTGGAGTGTTAATCTCCGTGTTCACCAGAGGGTC CACAGGGGTGAGAAGCCCTATAAATGTGAGGAATGTGGTAAGGGCTTCACTCAGGCTGCACATTTTCACA TCCATCAGAGAGTCCACACTGGAGAGAAACCCTACAAGTGTGATGTGTGTGGTAAGGGCTTCAGCCACAA TTCACCATTAATATGCCATCGGAGAGTCCACACAGGAGAGAAGCCATACAAGTGTGAGGCGTGTGGGAAA GGCTTTACCCGTAATACAGATCTGCATATTCATTTCAGAGTTCACACGGGAGAGAAACCCTATAAATGTA AGGAGTGTGGTAAGGGCTTCAGTCAGGCTTCAAATCTTCAAGTCCATCAGAATGTCCACACTGGGGAGAA ACGATTCAAGTGTGAAACGTGTGGGAAGGGCTTCAGTCAGTCCTCAAAGCTTCAAACCCATCAGCGAGTC CACACTGGAGAGAAACCATATAGATGTGATGTGTGTGGTAAGGACTTCAGTTATAGTTCAAATCTTAAAC TACACCAAGTAATTCACACTGGAGAAAAACCATATAAATGTGAGGAATGTGGGAAGGGCTTCAGTTGGAG ATCAAATCTTCATGCACATCAAAGAGTTCACTCAGGAGAAAAACCCTATAAATGTGAGCAGTGTGATAAG AGCTTCAGTCAGGCCATAGATTTTCGGGTACATCAGAGAGTCCATACTGGAGAGAAGCCATACAAATGTG GTGTCTGTGGTAAGGGCTTCAGTCAGTCCTCTGGTCTTCAATCCCATCAGAGAGTCCACACGGGGGAAAA GCCATACAAATGTGATGTGTGTGGAAAGGGCTTTAGATACAGTTCGCAGTTTATATACCATCAGAGAGGC CACACTGGAGAAAAACCTTACAAATGTGAAGAGTGTGGGAAAGGCTTTGGTAGGAGCTTGAATCTTCGCC ATCATCAGAGGGTCCACACGGGAGAGAAACCCCATATATGTGAGGAGTGTGGTAAGGCCTTCAGTCTCCC CTCAAATCTTCGAGTCCACCTGGGTGTTCACACCAGGGAAAAACTCTTTAAATGTGAAGAGTGTGGTAAA GGCTTCAGTCAGAGTGCACGTCTTGAAGCCCATCAGAGAGTCCACACTGGAGAAAAACCATACAAATGTG ACATATGTGATAAGGACTTCCGTCACCGTTCACGTCTTACATATCATCAGAAAGTCCATACTGGTAAAAA GCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239466 representing NM_001289167 Red=Cloning site Green=Tags(s) MVENFKNLVAVGHLPFQPDMVSQLEAEEKLWMMETETQRSSKHQNKMETLQKFALKYLSNQELSCWQIWK QVASELTRCLQGKSSQLLQGDSIQVSENENNIMNPKGDSSIYIENQEFPFWRTQHSCGNTYLSESQIQSR GKQIDVKNNLQIHEDFMKKSPFHEHIKTDTEPKPCKGNEYGKIISDGSNQKLPLGEKPHPCGECGRGFSY SPRLPLHPNVHTGEKCFSQSSHLRTHQRIHPGEKLNRCHESGDCFNKSSFHSYQSNHTGEKSYRCDSCGK GFSSSTGLIIHYRTHTGEKPYKCEECGKCFSQSSNFQCHQRVHTEEKPYKCEECGKGFGWSVNLRVHQRV HRGEKPYKCEECGKGFTQAAHFHIHQRVHTGEKPYKCDVCGKGFSHNSPLICHRRVHTGEKPYKCEACGK GFTRNTDLHIHFRVHTGEKPYKCKECGKGFSQASNLQVHQNVHTGEKRFKCETCGKGFSQSSKLQTHQRV HTGEKPYRCDVCGKDFSYSSNLKLHQVIHTGEKPYKCEECGKGFSWRSNLHAHQRVHSGEKPYKCEQCDK SFSQAIDFRVHQRVHTGEKPYKCGVCGKGFSQSSGLQSHQRVHTGEKPYKCDVCGKGFRYSSQFIYHQRG HTGEKPYKCEECGKGFGRSLNLRHHQRVHTGEKPHICEECGKAFSLPSNLRVHLGVHTREKLFKCEECGK GFSQSARLEAHQRVHTGEKPYKCDICDKDFRHRSRLTYHQKVHTGKKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289167
ORF Size	2244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289167.1, NP_001276096.1
RefSeq Size	3032 bp
RefSeq ORF	2247 bp
Locus ID	7770
UniProt ID	Q86WZ6
Protein Families	Transcription Factors
MW	86.3 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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