TLE 1 (TLE1) (NM_001303103) Human Tagged ORF Clone
CAT#: RC239546
- TrueORF®
TLE1 (myc-DDK-tagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), transcript variant 1
ORF Plasmid: tGFP
"NM_001303103" in other vectors (2)
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CNY 5,800.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ESG; ESG1; GRG1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239546 representing NM_001303103
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCGG AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATG TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGC AGAATTGAATGCCATCATCGGGGTACGTGGCCTACCAGGTCTACCTCCTACACAGCAGCAGTTGCAAGCT CAGCATCTTTCTCATGGCCACGGACCCCCAGTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTG GAATCCCGCCCCTCGGGGGCAGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTT GGCAATAAAAGATGACAAGAAGCACCACGATGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAAT TCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAACGCAGAAATGGACCTGAATTTTCCAATGACA TCAAGAAAAGGAAGGTGGATGATAAGGACTCCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAA CTTAGTTGTGGATGTGTCTAATGAGGACCCTTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAA AATGGAATCGACAAAAATCGCCTGCTAAAGAAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGG CAAGTTCCACTTCTTTGAAATCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATC CAGCACACCAACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGGT CTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAAGCGGCAGCTGGCTTGAGGACACCCCTGGCAG TGCCCGGCCCATATCCTGCTCCTTTTGGGATGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCC AGGCGCTGCCTACGCCAGTTTACACAACATGTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTG GTGGCCTACGGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTC CAAACCTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCA GCCTGTCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACC CTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGCAACCCCACGAGACACGTGTACACAGGCGGGA AGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCT GAACAGAGACAATTATATCCGTTCCTGTAAATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAA GCCAGTACTTTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGG CCCCCGCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAA CATCGCTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGC TGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACGGGTGGTTTGGACAACACAGTCAGGTCCTGGG ACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCC CACCGGGGAGTGGCTGGCAGTGGGCATGGAGAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGAC AAGTACCAGCTGCACCTGCATGAGAGCTGCGTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTG TGAGTACTGGAAAAGATAACCTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAA AGAGTCCTCGTCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGAC AAGAAGGCTACAGTCTATGAAGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239546 representing NM_001303103
Red=Cloning site Green=Tags(s) MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQLQA QHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSN SLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRE NGIDKNRLLKKDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPG LGKPPAIDPLVNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAV VAYGRSPMVGFDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINT LNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGE ASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGAS CIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPD KYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGD KKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001303103 |
ORF Size | 2340 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001303103.1, NP_001290032.1 |
RefSeq Size | 4167 bp |
RefSeq ORF | 2343 bp |
Locus ID | 7088 |
UniProt ID | Q04724 |
Protein Families | Transcription Factors |
MW | 84.6 kDa |
Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG239546 | TLE1 (tGFP-tagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), transcript variant 1 |
CNY 6,180.00 |
|
SC337440 | TLE1 (untagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), transcript variant 1 |
CNY 7,410.00 |