SECISBP2 (NM_001282689) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SBP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239549 representing NM_001282689 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTAGAGTCCTCAGAAGCATGTGTCTTCCCCAGCTCTGCAGCCACATACTATCCGTTTGTTCAGGAA CCACCAGTGACAGAAATGTTTACTCAGTGCCTGGCTCCCAGTATCTTTATAACCAACCCAGTTGTTACCG AGGTTTTCAAACAGTGAAGCATCGAAATGAGAACACATGCCCTCTCCCACAAGAAATGAAAGCTCTGTTT AAGAAGAAAACCTATGATGAGAAAAAAACGTATGATCAGCAAAAGTTTGACAGTGAAAGGGCTGATGGAA CTATATCATCTGAGATAAAATCAGCTAGAGGTTCACATCATTTGTCCATTTACGCTGAGAATAGTTTGAA ATCAGATGGTTACCATAAGCGAACAGACAGGAAATCCAGAATCATTGCAAAAAATGTATCTACCTCCAAA CCTGAGTTTGAATTTACCACACTGGACTTTCCTGAACTGCAAGGTGCAGAGAACAATATGTCAGAGATAC AGAAGCAACCCAAGTGGGGACCTGTCCACTCTGTCTCTACCGACATTTCTCTTCTAAGAGAAGTAGTAAA ACCAGCTGCAGTGTTATCAAAGGGTGAAATAGTGGTGAAAAATAACCCAAATGAATCTGTAACTGCTAAT GCCGCTACCAATTCTCCTTCATGTACAAGAGAGTTATCTTGGACACCAATGGGTTATGTTGTTCGACAGA CATTATCTACAGAACTGTCAGCAGCCCCTAAAAATGTTACTTCTATGATAAACTTAAAGACCATTGCTTC ATCAGCAGATCCTAAAAATGTTAGTATACCATCTTCTGAAGCTTTATCTTCGGATCCTTCCTACAACAAA GAAAAACACATTATTCATCCTACCCAAAAGTCTAAAGCATCACAAGGTAGTGACCTTGAACAAAATGAAG CCTCAAGAAAGAATAAGAAAAAGAAAGAAAAATCTACATCAAAATATGAAGTCCTGACAGTTCAAGAGCC TCCAAGGATTGAAGATGCCGAGGAATTTCCCAACCTGGCAGTTGCATCTGAAAGAAGAGACAGAATAGAG ACACCGAAATTTCAATCTAAGCAGCAGCCACAGGATAATTTTAAAAATAATGTAAAGAAGAGCCAGCTTC CAGTGCAGTTGGACTTGGGGGGCATGCTGACAGCCCTGGAGAAGAAGCAGCACTCTCAGCATGCAAAGCA GTCCTCCAAACCAGTGGTAGTCTCAGTTGGAGCAGTGCCAGTCCTTTCCAAAGAATGTGCATCAGGGGAG AGAGGCCGCCGCATGAGTCAAATGAAGACCCCGCACAATCCCTTGGACTCCAGCGCCCCACTGATGAAGA AAGGGAAGCAGAGGGAGATCCCCAAGGCCAAGAAGCCAACCTCACTGAAGAAGATTATTTTGAAAGAACG GCAAGAGAGAAAGCAGCGTCTCCAAGAAAATGCTGTGAGTCCAGCTTTTACCAGTGATGACACACAAGAT GGAGAGAGTGGTGGTGATGACCAGTTTCCCGAGCAGGCAGAGCTGTCAGGGCCAGAGGGGATGGACGAAC TGATCTCCACTCCTTCGGTTGAGGACAAGTCTGAAGAGCCACCAGGCACAGAGCTCCAGAGGGACACAGA GGCCTCCCACCTTGCTCCCAATCACACCACCTTCCCTAAGATCCACAGCCGCAGATTCAGGGATTACTGC AGCCAGATGCTTAGTAAAGAAGTGGATGCTTGTGTTACCGACCTACTCAAAGAACTGGTCCGTTTCCAAG ACCGTATGTACCAGAAAGATCCAGTCAAGGCCAAGACTAAACGTCGACTTGTGTTGGGGTTGAGGGAGGT TCTCAAACACCTGAAGCTCAAAAAACTGAAATGTGTCATTATTTCTCCCAACTGTGAGAAGATACAGTCA AAAGGTGGGCTGGATGACACTTTGCACACAATTATTGATTATGCCTGTGAGCAGAACATTCCCTTTGTGT TTGCTCTCAACCGCAAAGCTCTGGGGCGCAGTTTGAATAAGGCAGTTCCTGTCAGTGTGGTGGGGATCTT CAGCTATGATGGGGCCCAGGATCAGTTCCACAAGATGGTTGAGCTGACAGTGGCGGCCCGACAGGCGTAC AAGACCATGCTGGAGAATGTGCAGCAGGAGCTGGTGGGAGAGCCCAGGCCTCAGGCACCTCCCAGCCTAC CCACACAGGGCCCCAGCTGCCCTGCAGAAGATGGCCCCCCAGCCCTGAAAGAAAAAGAAGAGCCACACTA CATTGAAATCTGGAAAAAACATCTGGAAGCATACAGTGGATGTACCCTGGAGCTAGAAGAATCCTTGGAG GCTTCAACCTCTCAAATGATGAATTTGAATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239549 representing NM_001282689 Red=Cloning site Green=Tags(s) MVRVLRSMCLPQLCSHILSVCSGTTSDRNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTCPLPQEMKALF KKKTYDEKKTYDQQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRIIAKNVSTSK PEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESVTAN AATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNK EKHIIHPTQKSKASQGSDLEQNEASRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRDRIE TPKFQSKQQPQDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECASGE RGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDTQD GESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFRDYC SQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQS KGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAY KTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTLELEESLE ASTSQMMNLNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282689
ORF Size	2343 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282689.2
RefSeq Size	3414 bp
RefSeq ORF	2346 bp
Locus ID	79048
UniProt ID	Q96T21
MW	87.8 kDa
Gene Summary	The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]

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