Rabenosyn 5 (RBSN) (NM_001302378) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rabenosyn-5; ZFYVE20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239555 representing NM_001302378 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTCTGGACGACCCAGGGGAAGTGAGGGAGGGCTTCCTCTGCCCTCTGTGCCTGAAGGATCTGC AGTCTTTCTATCAGCTTCACTCACATTACGAGGAAGAACACTCAGGGGAAGACCGTGATGTCAAAGGGCA AATTAAAAGTCTTGTCCAGAAGGCTAAAAAAGCAAAGGACAGGTTGTTGAAACGAGAAGGGGATGATCGA GCAGAGTCAGGGACCCAAGGATATGAGTCTTTCAGCTATGGAGGGGTTGATCCTTACATGTGGGAACCCC AGGAGCTTGGTGCTGTGAGAAGCCATCTTTCCGACTTCAAAAAACACCGAGCTGCTAGAATTGACCACTA TGTTGTGGAAGTCAATAAACTAATAATCAGGTTAGAGAAGCTCACTGCATTTGACAGAACAAATACTGAG TCTGCAAAGATTCGAGCAATAGAAAAGTCTGTGGTGCCTTGGGTCAACGACCAGGATGTCCCTTTCTGTC CAGACTGTGGGAATAAGTTCAGCATCCGGAACCGCCGCCACCACTGCCGCCTCTGCGGGTCTATTATGTG CAAGAAGTGTATGGAGCTCATCAGCCTTCCCTTGGCAAACAAGCTCACCAGTGCCAGCAAGGAGTCCCTG AGCACCCACACCAGCCCCAGCCAGTCACCCAACAGTGTCCATGGCTCCCGCCGAGGCAGCATCAGCAGCA TGAGCAGTGTCAGCTCGGTCCTGGATGAGAAGGACGATGACCGGATCCGCTGCTGTACACACTGCAAGGA CACGCTGCTCAAGAGAGAGCAGCAGATTGATGAGAAGGAGCACACACCTGACATCGTGAAGCTCTACGAG AAATTACGACTTTGCATGGAGAAAGTTGACCAGAAAGCTCCAGAATACATCAGGATGGCAGCATCATTAA ATGCTGGGGAGACAACCTACAGTCTGGAACATGCCAGTGACCTTCGAGTGGAAGTGCAGAAAGTGTATGA GCTGATAGACGCTTTAAGTAAGAAGATCTTAACCTTGGGCTTGAACCAGGACCCTCCACCACATCCAAGC AATTTGCGGCTGCAGAGAATGATCAGATACTCAGCTACACTTTTTGTGCAGGAAAAGTTGCTTGGTTTGA TGTCACTGCCAACCAAAGAACAGTTTGAGGAACTGAAAAAGAAAAGGAAGGAGGAAATGGAGAGGAAGAG GGCCGTGGAGAGACAAGCTGCCCTGGAGTCCCAGCGAAGGCTTGAGGAAAGGCAGAGTGGCCTGGCTTCT CGAGCGGCCAACGGGGAGGTGGCATCTCTCCGCAGGGGCCCTGCCCCCTTGAGAAAGGCTGAGGGCTGGC TCCCACTGTCAGGAGGTCAGGGGCAGAGTGAGGACTCAGACCCGCTCCTCCAGCAGATCCACAACATCAC ATCATTCATCAGGCAGGCCAAGGCCGCGGGCCGCATGGATGAAGTGCGCACTCTGCAGGAGAACCTGCGG CAGCTGCAGGACGAGTATGACCAGCAGCAGACAGAGAAGGCCATCGAGCTGTCCCGGAGGCAGGCTGAGG AGGAGGACCTGCAGCGGGAACAGCTGCAGATGTTGCGTGAACGGGAGTTGGAACGAGAAAGGGAGCAGTT TCGGGTGGCATCCCTGCACACACGGACTCGGTCCCTGGACTTCAGAGAAATCGGCCCTTTTCAGCTGGAG CCCAGCAGAGAGCCTCGCACCCACCTTGCTTATGCTTTGGATCTAGGCTCTTCCCCAGTTCCAAGCAGCA CAGCTCCCAAGACCCCTTCACTTAGCTCAACTCAACCCACCAGAGTGTGGTCTGGGCCCCCAGCCGTTGG CCAGGAGCGCTTACCCCAGAGCAGCATGCCACAGCAACATGAGGGGCCCTCCTTAAACCCCTTTGATGAG GAAGACCTCTCCAGCCCCATGGAAGAGGCCACTACTGGTCCTCCTGCTGCAGGGGTTTCCTTAGACCCTT CAGCCCGCATCCTGAAAGAGTACAATCCTTTCGAGGAAGAGGACGAGGAGGAGGAAGCAGTGGCAGGGAA TCCATTCATTCAGCCAGACAGCCCAGCTCCTAACCCCTTCAGTGAGGAAGACGAACATCCCCAGCAGAGG CTCTCAAGCCCTCTGGTTCCTGGTAACCCCTTTGAGGAACCCACCTGTATCAACCCCTTTGAGATGGACA GTGACAGTGGGCCAGAGGCTGAGGAGCCCATAGAGGAAGAGCTCCTCCTGCAGCAGATCGATAACATCAA GGCATACATCTTTGATGCCAAGCAGTGCGGCCGCCTGGATGAGGTAGAGGTGCTGACAGAGAATCTGCGG GAGCTGAAGCACACCCTGGCCAAGCAGAAGGGGGGCACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239555 representing NM_001302378 Red=Cloning site Green=Tags(s) MASLDDPGEVREGFLCPLCLKDLQSFYQLHSHYEEEHSGEDRDVKGQIKSLVQKAKKAKDRLLKREGDDR AESGTQGYESFSYGGVDPYMWEPQELGAVRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTE SAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKESL STHTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKEHTPDIVKLYE KLRLCMEKVDQKAPEYIRMAASLNAGETTYSLEHASDLRVEVQKVYELIDALSKKILTLGLNQDPPPHPS NLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKEEMERKRAVERQAALESQRRLEERQSGLAS RAANGEVASLRRGPAPLRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLR QLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLE PSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDE EDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQR LSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLR ELKHTLAKQKGGTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302378
ORF Size	2352 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302378.2
RefSeq Size	6540 bp
RefSeq ORF	2355 bp
Locus ID	64145
UniProt ID	Q9H1K0
Protein Families	Transcription Factors
Protein Pathways	Endocytosis
MW	89.3 kDa
Gene Summary	This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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