SLC9A3 (NM_001284351) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DIAR8; NHE-3; NHE3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239640 representing NM_001284351 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGACTCGGGGCCCGGGGCCCCGACCGGGGGCTGCTGCTGGCGCTGGCGCTGGGCGGGCTGGCGC GGGCCGGGGGCGTCGAGGTGGAGCCCGGCGGCGCGCACGGCGAGAGCGGGGGCTTCCAGGTGGTCACCTT CGAGTGGGCCCACGTGCAGGATCCCTACGTCATCGCGCTCTGGATCCTCGTGGCCAGCTTGGCCAAGATC GGGTTCCACCTGTCCCACAAGGTCACCAGCGTGGTTCCCGAGAGCGCCCTGCTCATCGTGCTGGGCCTGG TGCTGGGCGGCATCGTCTGGGCGGCCGACCACATCGCGTCCTTCACACTGACGCCCACCGTCTTCTTCTT CTACCTGCTGCCCCCCATCGTGCTGGACGCCGGCTACTTCATGCCCAACCGCCTCTTCTTCGGCAACCTG GGGACCATCCTGTTGTACGCCGTCGTGGGTACCGTGTGGAACGCGGCCACCACCGGGCTGTCCCTCTACG GCGTCTTCCTCAGTGGGCTCATGGGCGACCTGCAGATTGGGCTGCTGGACTTCCTCCTGTTTGGCAGCCT CATGGCGGCTGTGGACCCGGTGGCCGTCCTGGCCGTGTTTGAGGAGGTCCATGTCAACGAGGTCCTGTTC ATCATCGTCTTCGGGGAGTCGCTGCTGAACGACGCAGTCACCGTGGTTCTGTACAATGTGTTTGAATCTT TCGTGGCGCTGGGAGGTGACAACGTGACTGGCGTGGACTGCGTGAAGGGCATAGTGTCCTTCTTCGTGGT GAGCCTGGGGGGCACGCTGGTGGGGGTGGTCTTCGCCTTCCTGCTGTCGCTGGTGACGCGCTTCACCAAG CATGTGCGTATCATCGAGCCCGGCTTCGTGTTCATCATCTCCTACCTGTCCTACCTGACGTCCGAGATGC TGTCGCTGTCGGCCATCCTCGCCATCACCTTCTGTGGCATCTGCTGTCAGAAGTATGTGAAGGCCAACAT CTCGGAGCAGTCGGCCACCACCGTGCGCTACACCATGAAGATGCTGGCCAGCAGCGCCGAGACCATCATC TTCATGTTCCTGGGTATCTCGGCCGTGAACCCGTTCATCTGGACCTGGAACACGGCCTTCGTGCTCCTGA CGCTGGTCTTCATCTCCGTGTACCGGGCCATCGGTGTGGTCCTGCAGACCTGGCTTCTGAACCGCTACCG CATGGTGCAGCTGGAGCCCATTGACCAGGTGGTCCTGTCCTACGGGGGCCTGCGCGGGGCCGTGGCCTTT GCCCTGGTGGTGCTTCTGGATGGAGACAAGGTCAAGGAGAAGAACCTGTTCGTCAGCACCACCATCATCG TAGTGTTCTTCACCGTCATCTTCCAGTGGCTGAAGGTGAAGAGGAGCGAGCACCGGGAACCTCGGCTCAA CGAGAAGCTGCACGGGCGCGCTTTCGACCACATCCTCTCGGCCATCGAGGACATATCCGGACAGATCGGG CACAATTATCTCAGAGACAAGTGGTCCCACTTCGACAGGAAGTTCCTCAGCAGGGTCCTCATGAGACGGT CGGCCCAGAAGTCTCGAGACCGGATCCTGAATGTCTTCCACGAGCTGAACCTGAAGGATGCCATCAGCTA CGTGGCTGAGGGAGAGCGCCGCGGGTCCCTGGCCTTCATCCGCTCCCCCAGCACCGACAACGTGGTCAAC GTGGACTTCACGCCACGATCGTCCACCGTGGAGGCCTCTGTCTCCTACCTCCTGAGAGAAAATGTCAGCG CTGTCTGCCTGGACATGCAGTCTCTGGAGCAGCGACGGCGGAGCATCCGGGACGCGGAGGACATGGTCAC GCACCACACGCTACAGCAGTACCTGTACAAGCCGCGGCAGGAGTACAAGCATCTGTACAGCCGACACGAG CTCACGCCCACGGAGGACGAGAAACAGGACCGGGAAATCTTCCACAGGACCATGCGGAAGCGCCTGGAGT CCTTCAAGTCGACCAAGCTGGGGCTCAACCAGAACAAGAAGGCGGCCAAGCTGTACAAGCGGGAGCGTGC CCAGAAGCGGAGAAACAGCAGCATCCCCAATGGGAAGCTGCCCATGGAGAGCCCTGCGCAGAATTTCACC ATCAAGGAGAAAGACTTGGAACTTTCAGACACCGAGGAGCCCCCCAACTATGATGAGGAGATGAGTGGGG GGATCGAGTTCCTGGCTAGTGTCACCAAGGACACAGCGTCCGACTCCCCTGCAGGAATTGACAACCCTGT GTTTTCTCCGGACGAGGCCCTGGACCGCAGCCTCCTGGCCAGGCTGCCGCCCTGGCTGTCTCCCGGGGAG ACGGTGGTCCCCTCGCAGAGGGCCCGCACGCAGATTCCCTACTCTCCCGGCACCTTCTGCCGCCTGATGC CCTTCCGCCTCAGCAGCAAGTCCGTGGACTCCTTCCTGCAGGCAGACGGCCCCGAGGAGCGGCCCCCCGC CGCCCTCCCCGAGTCCACACACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239640 representing NM_001284351 Red=Cloning site Green=Tags(s) MWGLGARGPDRGLLLALALGGLARAGGVEVEPGGAHGESGGFQVVTFEWAHVQDPYVIALWILVASLAKI GFHLSHKVTSVVPESALLIVLGLVLGGIVWAADHIASFTLTPTVFFFYLLPPIVLDAGYFMPNRLFFGNL GTILLYAVVGTVWNAATTGLSLYGVFLSGLMGDLQIGLLDFLLFGSLMAAVDPVAVLAVFEEVHVNEVLF IIVFGESLLNDAVTVVLYNVFESFVALGGDNVTGVDCVKGIVSFFVVSLGGTLVGVVFAFLLSLVTRFTK HVRIIEPGFVFIISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTMKMLASSAETII FMFLGISAVNPFIWTWNTAFVLLTLVFISVYRAIGVVLQTWLLNRYRMVQLEPIDQVVLSYGGLRGAVAF ALVVLLDGDKVKEKNLFVSTTIIVVFFTVIFQWLKVKRSEHREPRLNEKLHGRAFDHILSAIEDISGQIG HNYLRDKWSHFDRKFLSRVLMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNVVN VDFTPRSSTVEASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKHLYSRHE LTPTEDEKQDREIFHRTMRKRLESFKSTKLGLNQNKKAAKLYKRERAQKRRNSSIPNGKLPMESPAQNFT IKEKDLELSDTEEPPNYDEEMSGGIEFLASVTKDTASDSPAGIDNPVFSPDEALDRSLLARLPPWLSPGE TVVPSQRARTQIPYSPGTFCRLMPFRLSSKSVDSFLQADGPEERPPAALPESTHM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284351
ORF Size	2475 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284351.3
RefSeq Size	2750 bp
RefSeq ORF	2478 bp
Locus ID	6550
UniProt ID	P48764
Protein Families	Druggable Genome, Transmembrane
MW	92.4 kDa
Gene Summary	The protein encoded by this gene is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell. Defects in this gene are a cause of congenital secretory sodium diarrhea. Pseudogenes of this gene exist on chromosomes 10 and 22. [provided by RefSeq, Mar 2016]

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