RNF190 (MARCH10) (NM_001288779) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MARCH-X; MARCH10; RNF190
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239671 representing NM_001288779 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCATA AGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAACG CGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAACAG AGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGTGACT CCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGGAAAGC AGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGAGTCAGAGCCGACTTGGTGGAATAAACCGGAAACA GACCTCGGAGGACCTTCTGGCTGGTGCAGTCTGGGGAAGAAAGGTCCCATGAGCAGAAGCATCCCTCCCT GTGAAGCAGACCAAGCACCAATGGTTTTATTAAGAAAGAGGAAGCCAAACCTGAGGAGATTTACAGTCAG CCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGAAGCAGACAGAAACAGCAGTGGCCTGCAAAGGTG CCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAAGAAGGCCTGATGTGTAACACTAAGCTGAAGAGGC CAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCACAGCCAATGACTGAGAACGCCCCTGATAGAGCCAA AAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAGCTGCATCCAGCCCTGTCCCAGGCCTTCCAAGGAAAA AATAGTCCTCAAGTATTGAGTGAGTTCTCGGGGCCACCACTCACACCCACCACTGTAGGAGGGCCAAGAA AGGCATCATTTAGGTTCCGAGATGAAGACTTTTATTCCATTTTGTCATTGAACAGCAGAAGAGAAAGCGA TGACACTGAAGAGGAAACCCAGTCTGAAGAATGTCTGTGGGTTGGTGTGCGCTCACCCTGTTCTCCTTCC CATCACAAAAGAAGTAGATTTGGGGGGACATCGACCCCTCAGGCCAAAAATAAAAATTTTGAAGAAAATG CTGAAAATTGCAGAGGTCATTCTTCAAGAAGAAGTGAGCCCAGTCATGGCTCATTGAGAATAAGCAATGC AATGGAGCCAGCAACAGAGCGGCCTTCTGCTGGGCAAAGGTTGTCCCAAGACCCCGGGCTGCCTGATAGG GAATCTGCTACAGAGAAGGACAGAGGTGGCAGTGAAAATGCGAAAAAGAGCCCTCTTTCGTGGGACACCA AATCCGAGCCCAGACAAGAGGTTGGTGTCAATGCTGAAAATGTATGGAGTGATTGTATTTCTGTGGAACA TAGGCCTGGCACCCATGATTCTGAAGGATACTGGAAAGACTATTTAAACAGTTCTCAAAATTCTCTTGAC TACTTTATTTCTGGCAGACCAATATCTCCAAGATCATCAGTGAATTCATCCTATAACCCTCCTGCATCAT TCATGCATTCTGCTCTGAGAGATGATATTCCAGTAGACTTGTCAATGTCATCGACTTCAGTTCACAGCTC AGACTCAGAGGGAAACTCAGGGTTTCATGTTTGCCAACCACTGTCTCCCATTAGAAATAGGACTCCATTT GCCAGTGCCGAAAACCATAATTATTTCCCAGTAAACAGTGCACACGAATTTGCTGTCAGGGAAGCAGAAG ATACTACTTTAACAAGCCAGCCTCAGGGGGCTCCACTATATACAGATCTCTTACTAAATCCACAGGGCAA TTTGTCCTTAGTGGATTCTTCCAGCTCCTCTCCATCAAGAATGAACTCAGAAGGCCATTTGCATGTGTCT GGGTCTCTGCAAGAAAATACACCATTTACTTTCTTTGCAGTGTCTCATTTCCCAAATCAAAATGATAATG GGAGCAGGATGGCAGCCTCTGGTTTCACAGATGAAAAGGAAACCAGTAAGATAAAGGCAGACCCTGAGAA ACTCAAGAAATTGCAAGAAAGTCTCCTGGAGGAGGACTCCGAGGAGGAGGGAGACTTGTGTCGCATCTGT CAGATAGCCGGGGGTTCCCCAAGCAACCCCCTCCTGGAGCCTTGCGGCTGTGTGGGAAGCCTGCAGTTTG TTCATCAAGAGTGCCTGAAAAAGTGGCTGAAAGTGAAAATAACATCAGGAGCAGATCTTGGTGCCGTGAA GACCTGTGAGATGTGTAAGCAAGGCCTGCTGGTTGACCTGGGTGACTTTAACATGATTGAGTTCTACCAG AAGCACCAGCAATCTCAGGCACAAAACGAGCTGATGAATTCAGGCTTGTACCTGGTGCTGCTGCTTCACC TCTATGAGCAGAGGTTTGCAGAACTCATGAGGCTCAACCACAACCAGGTGGAAAGAGAGAGGTTGTCAAG AAATTACCCACAACCCAGAACAGAGGAAAATGAAAATTCGGAGTTGGGAGATGGAAATGAAGGCAGCATT TCTCAAAGCCAGGTCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239671 representing NM_001288779 Red=Cloning site Green=Tags(s) MLHDARDRQKFFSDVQYLRDMQHKVDSEYQACLRRQEYRRDPNEKKRDQFWGQETSFERSRFSSRSSSKQ SSSEEDALTEPRSSIKISAFKCDSKLPAIDQTSVKQKHKSTMTVRKAEKVDPSEPSPAESEPTWWNKPET DLGGPSGWCSLGKKGPMSRSIPPCEADQAPMVLLRKRKPNLRRFTVSPESHSPRASGDRSRQKQQWPAKV PVPRGADQVVQQEGLMCNTKLKRPNQERRNLVPSSQPMTENAPDRAKKGDPSAPSQSELHPALSQAFQGK NSPQVLSEFSGPPLTPTTVGGPRKASFRFRDEDFYSILSLNSRRESDDTEEETQSEECLWVGVRSPCSPS HHKRSRFGGTSTPQAKNKNFEENAENCRGHSSRRSEPSHGSLRISNAMEPATERPSAGQRLSQDPGLPDR ESATEKDRGGSENAKKSPLSWDTKSEPRQEVGVNAENVWSDCISVEHRPGTHDSEGYWKDYLNSSQNSLD YFISGRPISPRSSVNSSYNPPASFMHSALRDDIPVDLSMSSTSVHSSDSEGNSGFHVCQPLSPIRNRTPF ASAENHNYFPVNSAHEFAVREAEDTTLTSQPQGAPLYTDLLLNPQGNLSLVDSSSSSPSRMNSEGHLHVS GSLQENTPFTFFAVSHFPNQNDNGSRMAASGFTDEKETSKIKADPEKLKKLQESLLEEDSEEEGDLCRIC QIAGGSPSNPLLEPCGCVGSLQFVHQECLKKWLKVKITSGADLGAVKTCEMCKQGLLVDLGDFNMIEFYQ KHQQSQAQNELMNSGLYLVLLLHLYEQRFAELMRLNHNQVERERLSRNYPQPRTEENENSELGDGNEGSI SQSQVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288779
ORF Size	2538 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288779.2
RefSeq Size	3268 bp
RefSeq ORF	2541 bp
Locus ID	162333
UniProt ID	Q8NA82
MW	95.1 kDa
Gene Summary	MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

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