BRD8 (NM_001300961) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	p120; SMAP; SMAP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239701 representing NM_001300961 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCATCCGAGAGAAGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGGTAAGGG TATCAGTTAGCAGAGCAATCAAGCCCTTTGCAGAACCTGGCCGCCCTCCAGACTGGTTCTCTCAAAAACA TTGTGCTTCCCAGTACTCGGAGCTTTTAGAGACCACTGAGACACCAAAACGGAAACGAGGTGAAAAGGGA GAAGTGGTGGAAACTGTTGAAGATGTTATTGTTCGGAAATTGACTGCTGAGCGAGTTGAAGAACTAAAGA AAGTGATAAAGGAAACCCAGGAGAGATATAGACGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACA CATGGACAGCAGACTGGATGAGCTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCT GAAGTAAAGAGGAAGGCTACAGATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGT TACCCACTGTGATGGTTCGCTCTCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTT GACTCCAACCACTATGGAAGAGGCTACCTCTGGGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCAC CTGAACAGTACAGGTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGC AAACACCCAATACTGTTGCAGCCTCCCCTGCTGCATCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGC TGGTCCTACACAGTTCACCACACCTCTTGCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTAAACTT GTGCCACCCCCTGTAGAGTCTGTGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCAGCACCATCCT CTGCTCCGGCTGTCTCCACTACTGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTGTTCCCATGGA GGCTGTGGGGGATCCACATACTGTGACTGTTTCCATGGACAGCAGTGAAATATCCATGATCATCAATTCT ATCAAAGAAGAGTGTTTTCGATCAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATG GGAAGGAAGAATTAGATCTGGCTGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTT TGAGACTGTTGGAGACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTG GCAGCAGTGGAAGCAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCCCTGGG AGCATCCTATCCAGCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGGTCAAGCAAGA GAGACTGGACTTTGAGGAAACGGAAAACAAGGGAATACATGAACTGGTGGACATCAGGGAGCCCAGTGCA GAGATCAAGGTGGAACCTGCAGAACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTC CAGCCACAAGTATGGAGCCACCAGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGC TGGAGAGATTGTTGAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACA GAGGCATCCCCTGAAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTAGAGGCAG AGACTCAGCACAAGTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGAT AAAGGATGCCCCAGGTGAGGATGAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGAGGAGCCTAAG GAAGAGGATCAAGGAGAAGGCTACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATG GCTTCAGCATACACAATGCTACACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTC TTCACAGTTCTCTGTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATC ATGCTTGTATGGAGAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACAGATGACA TAGCACCTGGCTACCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAAACATAGAAAA TGGACTGATCCGAAGCACAGCTGAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGCTGTAATGTAC AATAGCTCAGACCATGATGTCTATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGC AATTCTTGGCCACGCAGTTGATTATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAG AGATTCTACCCGCAAACAGGATGCTTCAGAGAAGGACAGTGTCCCAATGGGCTCTCCTGCCTTCCTTCTC TCTCTCTTTGATGGAGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239701 representing NM_001300961 Red=Cloning site Green=Tags(s) MVHPREAMFSIFCHEKWRSKLVRVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKG EVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIATKKKLEEEEA EVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEEATSGVNESEMAVASGH LNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQFTTPLASFTTVASEPPVKL VPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGDPHTVTVSMDSSEISMIINS IKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDV AAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEETENKGIHELVDIREPSA EIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIVEADVAIGKGDETPLTNVKT EASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGEDEEEDGVSEAASLEEPK EEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAI MLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMY NSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLL SLFDGGTRGRRCAIEADMKMKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300961
ORF Size	2586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300961.2
RefSeq Size	3039 bp
RefSeq ORF	2589 bp
Locus ID	10902
UniProt ID	Q9H0E9
Protein Families	Druggable Genome, Transcription Factors
MW	94.4 kDa
Gene Summary	The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014]

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