PRKCBP1 (ZMYND8) (NM_001281781) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PRKCBP1; PRO2893; RACK7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239958 representing NM_001281781 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCCACAGAGCTTGGCTGAAGAGGAAATAAAAACAGAACAGGAGGTGGTAGAGGGCATGGATATCT CTACTCGCTCCAAAGATCCTGGCTCTGCAGAGAGAACAGCCCAGAAAAGAAAGTTCCCCAGCCCTCCACA TTCTTCCAATGGCCACTCGCCGCAGGACACATCAACAAGCCCCATTAAAAAGAAAAAGAAACCTGGCTTA CTGAACAGTAACAATAAGGAGCAGGATGGACGGAATGATTTCTACTGCTGGGTTTGTCACCGGGAAGGCC AAGTCCTTTGCTGTGAGCTCTGTCCCCGGGTTTATCACGCTAAGTGTCTGAGACTGACATCGGAACCAGA GGGGGACTGGTTTTGTCCTGAATGTGAGAAAATTACAGTAGCAGAATGCATCGAGACCCAGAGTAAAGCC ATGACAATGCTCACCATTGAACAGTTATCCTACCTGCTCAAGTTTGCCATTCAGAAAATGAAACAGCCAG GGACAGATGCATTCCAGAAGCCCGTTCCATTGGAACAGCACCCTGACTATGCGGAATACATCTTCCATCC AATGGACCTTTGTACATTGGAAAAGAATGCGAAAAAGAAAATGTATGGCTGCACAGAAGCCTTCCTGGCT GATGCAAAGTGGATTTTGCACAACTGCATCATTTATAATGGGGGAAATCACAAATTGACGCAAATAGCGA AAGTAGTCATCAAAATCTGTGAACATGAGATGAATGAAATCGAAGTATGTCCAGAATGTTATCTAGCTGC TTGCCAAAAACGAGATAACTGGTTTTGTGAGCCTTGTAGCAATCCACATCCTTTGGTCTGGGCCAAACTG AAGGGGTTTCCATTCTGGCCTGCAAAAGCTCTAAGGGATAAAGACGGGCAGGTCGATGCCCGATTCTTTG GACAACATGACAGGGCCTGGGTTCCAATAAATAATTGCTACCTCATGTCTAAAGAAATTCCTTTTTCTGT GAAAAAGACTAAGAGCATCTTCAACAGTGCCATGCAAGAGATGGAGGTTTACGTGGAGAACATCCGCAGG AAGTTTGGGGTTTTTAATTACTCTCCATTTAGGACACCCTACACACCCAACAGCCAGTATCAAATGCTGC TCGATCCCACCAACCCCAGCGCCGGCACTGCCAAGATAGACAAGCAGGAGAAGGTCAAGCTCAACTTTGA CATGACGGCATCCCCCAAGATCCTGATGAGCAAGCCTGTGCTGAGTGGGGGCACAGGCCGCCGGATTTCC TTGTCGGATATGCCGCGCTCCCCCATGAGCACAAACTCTTCTGTGCACACGGGCTCCGACGTGGAGCAGG ATGCTGAGAAGAAGGCCACGTCGAGCCACTTCAGTGCGAGCGAGGAGTCCATGGACTTCCTGGATAAGAG CACAGATCGAAGCAAAGCTGAGATGGATTTGAAGGAGCTGAGCGAGTCGGTCCAGCAACAGTCCACCCCT GTTCCTCTCATCTCTCCCAAGCGCCAGATTCGTAGCAGGTTCCAGCTGAATCTTGACAAGACCATAGAGA GTTGCAAAGCACAATTAGGCATAAATGAAATCTCGGAAGATGTCTATACGGCCGTAGAGCACAGCGATTC GGAGGATTCTGAGAAGTCAGATAGTAGCGATAGTGAGTATATCAGTGATGATGAGCAGAAGTCTAAGAAC GAGCCAGAAGACACAGAGGACAAAGAAGGTTGTCAGATGGACAAAGAGCCATCTGCTGTTAAAAAAAAGC CCAAGCCTACAAACCCAGTGGAGATTAAAGAGGAGCTGAAAAGCACGTCACCAGCCAGCGAGAAGGCAGA CCCTGGAGCAGTCAAGGACAAGGCCAGCCCTGAGCCTGAGAAGGACTTTTCCGAAAAGGCAAAACCTTCA CCTCACCCCATAAAGGATAAACTGAAGGGAAAAGATGAGACGGATTCCCCAACAGTCCATTTGGGCCTGG ACTCTGATTCAGAGAGCGAACTTGTCATAGATTTAGGAGAAGACCATTCTGGGCGGGAGGGTCGAAAAAA TAAGAAGGAACCCAAAGAACCATCTCCCAAACAGGATGTTGTAGGTAAAACTCCACCATCCACGACGGTG GGCAGCCATTCTCCCCCGGAAACACCGGTGCTCACCCGCTCTTCCGCCCAAACTTCCGCGGCTGGCGCCA CAGCCACCACCAGCACGTCCTCCACGGTCACCGTCACGGCCCCGGCCCCCGCCGCCACAGGAAGCCCAGT GAAAAAGCAGAGGCCGCTTTTACCGAAGGAGACTGCCCCGGCCGTGCAGCGGGTCGTGTGGAACTCATCA ACTGTCCAGCAGAAGGAGATCACACAGAGCCCATCCACGTCCACCATCACCCTGGTGACCAGCACACAGT CATCGCCCCTGGTCACCAGCTCGGGGTCCATGAGCACCCTTGTGTCCTCAGTCAACGCTGACCTGCCCAT CGCCACTGCCTCAGCTGATGTCGCCGCTGATATTGCCAAGTACACTAGCAAAATGATGGATGCAATAAAA GGAACAATGACAGAAATATACAACGATCTTTCTAAAAACACTACTGGAAGCACAATAGCTGAGATTCGCA GGCTGAGGATCGAGATAGAGAAGCTCCAGTGGCTGCACCAGCAAGAGCTCTCCGAAATGAAACACAACTT AGAGCTGACCATGGCGGAGATGCGGCAGAGCCTGGAGCAGGAGCGGGACCGGCTCATCGCCGAGGTGAAG AAGCAGCTGGAGTTGGAGAAGCAGCAGGCGGTGGATGAGACCAAGAAGAAGCAGTGGTGCGCCAACTGCA AGAAGGAGGCCATCTTTTACTGCTGTTGGAACACCAGCTACTGTGACTACCCCTGCCAGCAAGCCCACTG GCCTGAGCACATGAAGTCCTGCACCCAGTCAGCTACTGCTCCTCAGCAGGAAGCGGATGCTGAGGTGAAC ACAGAAACACTAAATAAGTCCTCCCAGGGGAGCTCCTCGAGCACACAATCAGCACCTTCAGAAACGGCCA GCGCCTCCAAAGAGAAGGAGACGTCAGCTGAGAAAAGCAAGGAGAGTGGCTCGACCCTTGACCTTTCTGG CTCCAGAGAGACGCCCTCCTCCATTCTCTTAGGCTCCAACCAAGGCTCTGACCATTCCCGGAGTAATAAA TCCAGTTGGAGCAGCAGTGATGAGAAGAGGGGATCGACACGTTCCGATCACAACACCAGTACCAGCACGA AGAGCCTCCTCCCGAAAGAGTCTCGGCTGGACACCTTCTGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239958 representing NM_001281781 Red=Cloning site Green=Tags(s) MHPQSLAEEEIKTEQEVVEGMDISTRSKDPGSAERTAQKRKFPSPPHSSNGHSPQDTSTSPIKKKKKPGL LNSNNKEQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLTSEPEGDWFCPECEKITVAECIETQSKA MTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAEYIFHPMDLCTLEKNAKKKMYGCTEAFLA DAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCPECYLAACQKRDNWFCEPCSNPHPLVWAKL KGFPFWPAKALRDKDGQVDARFFGQHDRAWVPINNCYLMSKEIPFSVKKTKSIFNSAMQEMEVYVENIRR KFGVFNYSPFRTPYTPNSQYQMLLDPTNPSAGTAKIDKQEKVKLNFDMTASPKILMSKPVLSGGTGRRIS LSDMPRSPMSTNSSVHTGSDVEQDAEKKATSSHFSASEESMDFLDKSTDRSKAEMDLKELSESVQQQSTP VPLISPKRQIRSRFQLNLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSDSSDSEYISDDEQKSKN EPEDTEDKEGCQMDKEPSAVKKKPKPTNPVEIKEELKSTSPASEKADPGAVKDKASPEPEKDFSEKAKPS PHPIKDKLKGKDETDSPTVHLGLDSDSESELVIDLGEDHSGREGRKNKKEPKEPSPKQDVVGKTPPSTTV GSHSPPETPVLTRSSAQTSAAGATATTSTSSTVTVTAPAPAATGSPVKKQRPLLPKETAPAVQRVVWNSS TVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIK GTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVK KQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVN TETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSDHSRSNK SSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281781
ORF Size	3264 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281781.3
RefSeq Size	5033 bp
RefSeq ORF	3267 bp
Locus ID	23613
UniProt ID	Q9ULU4
Protein Families	Druggable Genome, Transcription Factors
MW	121.3 kDa
Gene Summary	The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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