NUT (NUTM1) (NM_001284293) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C15orf55; FAM22H; NUT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240007 representing NM_001284293 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCAGCGGTCGAACCAAGATTTAAAGTTAGGTCCCTACCGCAAATTCAGCGCTCTTTCTTATGGTG CATCTGCATTGCCGGGACCGGATATGAGCATGAAACCTAGTGCCGCCCTGTCTCCATCCCCTGCACTTCC CTTTCTCCCACCAACTTCTGACCCACCAGACCACCCACCCAGGGAGCCACCTCCACAGCCCATCATGCCT TCAGTATTCTCTCCAGACAACCCTCTGATGCTCTCTGCTTTCCCCAGCTCACTGTTGGTGACAGGGGACG GGGGCCCTTGCCTCAGTGGGGCTGGGGCTGGCAAGGTCATTGTCAAAGTCAAGACAGAAGGGGGGTCAGC TGAGCCCTCTCAAACTCAGAACTTTATCCTTACTCAGACTGCCCTCAATTCGACTGCCCCGGGCACTCCC TGTGGAGGCCTTGAGGGTCCTGCACCTCCATTTGTGACAGCATCTAATGTGAAGACCATTCTGCCCTCTA AGGCTGTTGGTGTCAGCCAGGAGGGTCCTCCAGGCCTTCCGCCTCAGCCTCCACCACCAGTTGCTCAACT GGTCCCCATTGTGCCCCTGGAAAAAGCTTGGCCAGGGCCACATGGGACAACCGGGGAAGGAGGTCCTGTG GCCACTCTATCCAAGCCTTCCCTAGGTGACCGCTCCAAAATTTCCAAGGACGTTTATGAGAACTTCCGTC AGTGGCAGCGTTACAAAGCCTTGGCCCGGAGGCACCTATCCCAGAGTCCTGACACAGAAGCTCTTTCCTG TTTTCTTATCCCAGTGCTTCGTTCCCTGGCCCGGCTGAAGCCCACTATGACCCTGGAGGAGGGACTGCCA TTGGCTGTGCAGGAGTGGGAGCACACCAGCAACTTTGACCGGATGATCTTTTATGAGATGGCAGAAAGGT TCATGGAGTTTGAGGCTGAGGAGATGCAGATTCAGAACACACAGCTGATGAATGGGTCTCAGGGCCTGTC TCCTGCAACCCCTTTGAAACTTGATCCTCTAGGGCCCCTGGCCTCTGAGGTTTGCCAGCAGCCAGTGTAC ATTCCGAAGAAGGCAGCCTCCAAGACACGGGCCCCCCGCCGGCGTCAGCGTAAAGCCCAGAGACCTCCTG CTCCTGAGGCACCCAAGGAGATCCCACCAGAAGCTGTGAAGGAGTATGTTGACATCATGGAATGGCTGGT GGGGACTCACTTGGCCACTGGGGAGTCAGATGGAAAACAAGAGGAAGAAGGGCAGCAGCAGGAGGAGGAA GGGATGTATCCAGATCCAGGTCTCCTGAGCTACATCAATGAGCTGTGTTCTCAGAAGGTCTTTGTCTCCA AGGTGGAGGCTGTCATTCACCCTCAATTTCTGGCAGATCTGCTGTCCCCAGAAAAACAGAGAGATCCCTT GGCCTTAATTGAGGAGCTAGAGCAAGAAGAAGGACTCACTCTTGCCCAGCTGGTCCAGAAGCGACTCATG GCCTTGGAAGAGGAGGAAGATGCAGAGGCGCCTCCAAGTTTCAGTGGCGCTCAGTTGGACTCAAGTCCTT CTGGTTCTGTTGAGGATGAAGATGGGGATGGGCGGCTTCGGCCCTCACCTGGGCTTCAGGGGGCTGGGGG CGCCGCTTGCCTTGGAAAGGTTTCTTCTTCAGGAAAACGGGCAAGAGAAGTGCATGGTGGGCAGGAGCAA GCCCTAGATAGCCCCAGAGGGATGCACAGGGATGGGAACACTCTGCCATCCCCCAGCAGCTGGGACCTGC AGCCAGAACTTGCAGCTCCACAGGGAACTCCGGGACCCTTGGGTGTGGAGAGGAGAGGGTCTGGGAAGGT TATAAACCAGGTATCTCTACATCAGGATGGCCATCTAGGAGGCGCTGGGCCTCCTGGGCACTGCCTGGTG GCTGATAGGACTTCAGAGGCTCTGCCCCTTTGTTGGCAGGGAGGCTTCCAGCCTGAGAGCACTCCCAGTT TGGATGCTGGACTTGCAGAGCTGGCTCCTCTGCAAGGACAAGGGTTAGAAAAGCAAGTCCTGGGATTGCA GAAAGGACAACAAACAGGGGGTCGTGGAGTGCTTCCTCAAGGGAAGGAGCCTTTAGCAGTGCCCTGGGAA GGCTCTTCAGGAGCCATGTGGGGAGATGACAGAGGTACCCCCATGGCTCAGAGTTATGATCAGAATCCTT CCCCTAGAGCAGCTGGGGAGAGGGACGATGTCTGTCTCAGCCCAGGAGTTTGGCTGAGCAGTGAGATGGA TGCTGTAGGCTTGGAGCTGCCTGTACAAATAGAGGAGGTCATAGAGAGCTTCCAAGTTGAGAAGTGTGTA ACTGAGTATCAGGAAGGCTGCCAGGGACTGGGCTCCAGGGGCAACATTTCCCTGGGTCCTGGAGAAACCC TAGTACCTGGGGATACGGAGAGCAGTGTGATTCCCTGTGGAGGCACAGTTGCGGCAGCTGCCCTAGAAAA GAGAAACTATTGCAGCTTGCCAGGACCTTTGAGGGCCAACAGCCCACCCTTGAGGTCCAAAGAAAATCAA GAACAGAGCTGTGAAACCGTAGGGCATCCCAGTGATCTGTGGGCAGAAGGTTGCTTCCCATTGCTAGAAA GTGGTGATTCCACACTGGGGTCTTCCAAAGAAACCCTTCCACCCACATGCCAAGGCAATCTCCTTATCAT GGGGACTGAGGATGCCTCCTCCTTGCCTGAAGCCAGTCAAGAGGCAGGGAGCAGAGGCAATTCCTTTTCT CCTCTGTTGGAAACCATAGAACCTGTCAACATACTAGATGTTAAAGATGACTGTGGCCTCCAACTAAGGG TCAGCGAGGACACCTGCCCACTGAATGTTCATTCTTATGACCCCCAAGGAGAAGGCAGGGTGGATCCTGA TCTGTCCAAGCCTAAAAACCTTGCTCCTTTACAAGAGAGTCAGGAGTCTTACACAACTGGGACTCCCAAA GCAACATCTTCTCACCAGGGCCTTGGAAGCACTTTGCCTAGAAGGGGAACCAGGAATGCCATAGTTCCGA GAGAAACTTCTGTTAGTAAAACACACAGGTCAGCAGACAGGGCCAAAGGAAAGGAGAAAAAGAAAAAGGA AGCAGAGGAAGAGGATGAGGAACTCTCCAACTTTGCTTACCTCTTGGCCTCTAAACTTAGCCTCTCACCA AGGGAGCATCCCCTCAGTCCTCACCATGCCTCAGGAGGTCAGGGCAGCCAGAGAGCATCCCACCTGCTCC CTGCTGGAGCAAAAGGCCCCAGCAAACTTCCATATCCTGTTGCCAAGTCTGGGAAGCGAGCTCTAGCTGG AGGTCCAGCCCCTACTGAAAAGACACCCCACTCAGGAGCTCAACTTGGGGTCCCCAGGGAGAAACCCCTA GCTCTGGGAGTAGTTCGACCCTCACAGCCTCGTAAAAGGCGGTGTGACAGTTTTGTCACGGGCAGAAGGA AGAAACGACGTCGTAGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240007 representing NM_001284293 Red=Cloning site Green=Tags(s) MFQRSNQDLKLGPYRKFSALSYGASALPGPDMSMKPSAALSPSPALPFLPPTSDPPDHPPREPPPQPIMP SVFSPDNPLMLSAFPSSLLVTGDGGPCLSGAGAGKVIVKVKTEGGSAEPSQTQNFILTQTALNSTAPGTP CGGLEGPAPPFVTASNVKTILPSKAVGVSQEGPPGLPPQPPPPVAQLVPIVPLEKAWPGPHGTTGEGGPV ATLSKPSLGDRSKISKDVYENFRQWQRYKALARRHLSQSPDTEALSCFLIPVLRSLARLKPTMTLEEGLP LAVQEWEHTSNFDRMIFYEMAERFMEFEAEEMQIQNTQLMNGSQGLSPATPLKLDPLGPLASEVCQQPVY IPKKAASKTRAPRRRQRKAQRPPAPEAPKEIPPEAVKEYVDIMEWLVGTHLATGESDGKQEEEGQQQEEE GMYPDPGLLSYINELCSQKVFVSKVEAVIHPQFLADLLSPEKQRDPLALIEELEQEEGLTLAQLVQKRLM ALEEEEDAEAPPSFSGAQLDSSPSGSVEDEDGDGRLRPSPGLQGAGGAACLGKVSSSGKRAREVHGGQEQ ALDSPRGMHRDGNTLPSPSSWDLQPELAAPQGTPGPLGVERRGSGKVINQVSLHQDGHLGGAGPPGHCLV ADRTSEALPLCWQGGFQPESTPSLDAGLAELAPLQGQGLEKQVLGLQKGQQTGGRGVLPQGKEPLAVPWE GSSGAMWGDDRGTPMAQSYDQNPSPRAAGERDDVCLSPGVWLSSEMDAVGLELPVQIEEVIESFQVEKCV TEYQEGCQGLGSRGNISLGPGETLVPGDTESSVIPCGGTVAAAALEKRNYCSLPGPLRANSPPLRSKENQ EQSCETVGHPSDLWAEGCFPLLESGDSTLGSSKETLPPTCQGNLLIMGTEDASSLPEASQEAGSRGNSFS PLLETIEPVNILDVKDDCGLQLRVSEDTCPLNVHSYDPQGEGRVDPDLSKPKNLAPLQESQESYTTGTPK ATSSHQGLGSTLPRRGTRNAIVPRETSVSKTHRSADRAKGKEKKKKEAEEEDEELSNFAYLLASKLSLSP REHPLSPHHASGGQGSQRASHLLPAGAKGPSKLPYPVAKSGKRALAGGPAPTEKTPHSGAQLGVPREKPL ALGVVRPSQPRKRRCDSFVTGRRKKRRRSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284293
ORF Size	3450 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284293.1, NP_001271222.1
RefSeq Size	4017 bp
RefSeq ORF	3453 bp
Locus ID	256646
UniProt ID	Q86Y26
MW	123 kDa
Gene Summary	Plays a role in the regulation of proliferation. Regulates TERT expression by modulating SP1 binding to TERT promoter binding sites.[UniProtKB/Swiss-Prot Function]

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