PER3 (NM_001289863) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FASPS3; GIG13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240031 representing NM_001289863 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGGGGAAGCTCCTGGCCCCGGGAGACGGGGGGCTAAGGACGAGGCCCTGGGCGAAGAATCGG GGGAGCGGTGGAGCCCCGAGTTCCATCTGCAGAGGAAATTGGCGGACAGCAGCCACAGTGAACAGCAAGA TCGAAACAGAGTTTCTGAAGAACTTATCATGGTTGTCCAAGAAATGAAAAAATACTTCCCCTCGGAGAGA CGCAATAAACCAAGCACTCTAGATGCCCTCAACTATGCTCTCCGCTGTGTCCACAGCGTTCAAGCAAACA GTGAGTTTTTCCAGATTCTCAGTCAGAATGGAGCACCTCAGGCAGATGTGAGCATGTACAGTCTTGAGGA GCTGGCCACTATCGCTTCAGAACACACTTCCAAAAACACAGATACCTTTGTGGCAGTATTTTCATTTCTG TCTGGAAGGTTAGTGCACATTTCTGAACAGGCTGCTTTGATCCTGAATCGTAAGAAAGATGTCCTGGCGT CTTCTCACTTTGTTGACCTGCTTGCACCTCAAGACATGAGGGTATTCTACGCGCACACTGCCAGAGCTCA GCTTCCTTTCTGGAACAACTGGACCCAAAGAGCAGCTGCACGGTATGAATGTGCTCCGGTGAAACCTTTT TTCTGCAGGATCCGTGGAGGTGAAGACAGAAAGCAAGAGAAGTGTCACTCCCCATTCCGGATCATCCCCT ATCTGATTCATGTACATCACCCTGCCCAGCCAGAATTGGAATCGGAACCTTGCTGTCTCACTGTGGTTGA AAAGATTCACTCTGGTTATGAAGCTCCTCGGATCCCAGTGAATAAAAGAATCTTCACCACCACACACACC CCAGGGTGTGTTTTTCTTGAAGTAGATGAAAAAGCAGTGCCTTTGCTGGGTTACCTACCTCAGGACCTGA TTGGAACATCGATCCTAAGCTACCTGCACCCTGAAGATCGTTCTCTGATGGTTGCCATACACCAAAAAGT TTTGAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGATTTTGTACTCAAAACGGAGACTAC ATCATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGCCGGAAGATTTCTTTCATCATTGGTC GGCATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCTACCAAAATTAAAAAGATGAACGATAA TGACAAAGACATAACAGAATTACAAGAACAAATTTACAAACTTCTCTTACAGCCAGTTCACGTGAGCGTG TCCAGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAGCAGCTTGTCAGCATCGCCTCCTCCAGTG AGGCCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAGATGACCTTGCAGCAGGTCTATGCCAGTGT GAACAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAGTCAATGACCAAATCATCATTCAAGCCAGTG ACGGGGACACGCACAGAACCGAATGGTGGTGGTGAATGTAAGACCTTTACTTCCTTCCACCAAACACTGA AAAACAATAGTGTGTACACTGAGCCCTGTGAGGATTTGAGGAACGATGAGCACAGCCCATCCTATCAACA GATCAACTGTATCGACAGTGTCATCAGATACCTGAAGAGCTACAACATTCCAGCTTTGAAAAGAAAGTGT ATCTCCTGTACAAATACAACTTCTTCCTCCTCAGAAGAAGACAAACAGAACCACAAGGCAGATGATGTCC AAGCCTTACAAGCTGGTTTGCAAATCCCAGCCATACCTAAATCAGAAATGCCAACAAATGGACGGTCCAT AGACACAGGAGGAGGAGCTCCACAGATCCTGTCCACGGCGATGCTGAGCTTGGGGTCGGGCATAAGCCAA TGCGGTTACAGCAGCACCATTGTCCATGTCCCACCCCCAGAGACAGCCAGGGATGCTACCCTCTTCTGTG AGCCCTGGACCCTGAACATGCAGCCAGCCCCTTTGACCTCGGAAGAATTTAAACACGTGGGGCTCACAGC GGCTGTTCTGTCAGCGCACACCCAGAAGGAAGAGCAGAATTATGTTGATAAATTCCGAGAAAAGATCCTG TCATCACCCTACAGCTCCTATCTTCAGCAAGAAAGCAGGAGCAAAGCTAAATATTCATATTTTCAAGGAG ATTCTACTTCCAAGCAGACGCGGTCGGCCGGCTGCAGGAAAGGGAAGCACAAGCGGAAGAAGCTGCCGGA GCCGCCAGACAGCAGCAGCTCGAACACCGGCTCTGGTCCCCGCAGGGGAGCGCATCAGAACGCACAGCCC TGCTGCCCCTCCGCGGCCTCCTCTCCGCACACCTCGAGCCCGACCTTCCCACCTGCCGCCATGGTGCCCA GCCAGGCCCCTTACCTCGTCCCAGCTTTTCCCCTCCCAGCCGCGACCTCACCCGGAAGAGAATACGCAGC CCCCGGAACTGCACCGGAAGGCCTGCATGGGCTGCCCTTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTC CCTTTTCCTTACTTGGATACTTTTATGACCGTTTTCCTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGC CATCGTTTTTGCCATGTCCATTCCTGGGGGCGACAGCCTCTTCTGCGATATCACCCTCAATGTCGTCAGC AATGAGTCCAACTCTGGACCCACCCCCTTCAGTCACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCA CAAAGCGAGGGGCACCCGTTCATTACTTCGAGAAGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAAG AGATGCCCAGACCCTCTGAATCTCCAGATCAGATGAGAAGGAACACGTGCCCACAAACTGAGTATTGTGT TACAGGCAACAATGGCAGTGAGAGCAGTCCTGCTACTACCGGTGCACTGTCCACGGGGTCACCTCCCAGG GAGAATCCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATC CTACTGCCAGCACACTGTCCATGGGATTGCCTCCCAGCAGGACTCCATCCCATCCTACTGCCACTGTTCT GTCCACGGGGTCACCTCCCAGCGAATCCCCATCCAGAACTGGTTCAGCAGCATCAGGAAGCAGCGACAGC AGTATATACCTTACTAGTAGTGTTTATTCTTCTAAAATCTCCCAAAATGGGCAGCAATCTCAGGACGTAC AGAAAAAAGAAACATTTCCTAATGTCGCCGAAGAGCCCATCTGGAGAATGATACGGCAGACACCTGAGCG CATTCTCATGACATACCAGGTACCTGAGAGGGTTAAAGAAGTTGTACTAAAAGAAGACCTGGAAAAGCTA GAAAGTATGAGGCAGCAGCAGCCCCAGTTTTCTCATGGGCAAAAGGAGGAGCTGGCTAAGGTGTATAATT GGATTCAAAGCCAGACTGTCACTCAAGAAATCGACATTCAAGCCTGTGTCACTTGTGAAAATGAAGATTC AGCTGATGGTGCGGCCACATCCTGTGGTCAGGTTCTGGTAGAAGACAGCTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240031 representing NM_001289863 Red=Cloning site Green=Tags(s) MPRGEAPGPGRRGAKDEALGEESGERWSPEFHLQRKLADSSHSEQQDRNRVSEELIMVVQEMKKYFPSER RNKPSTLDALNYALRCVHSVQANSEFFQILSQNGAPQADVSMYSLEELATIASEHTSKNTDTFVAVFSFL SGRLVHISEQAALILNRKKDVLASSHFVDLLAPQDMRVFYAHTARAQLPFWNNWTQRAAARYECAPVKPF FCRIRGGEDRKQEKCHSPFRIIPYLIHVHHPAQPELESEPCCLTVVEKIHSGYEAPRIPVNKRIFTTTHT PGCVFLEVDEKAVPLLGYLPQDLIGTSILSYLHPEDRSLMVAIHQKVLKYAGHPPFEHSPIRFCTQNGDY IILDSSWSSFVNPWSRKISFIIGRHKVRTSPLNEDVFATKIKKMNDNDKDITELQEQIYKLLLQPVHVSV SSGYGSLGSSGSQEQLVSIASSSEASGHRVEETKAEQMTLQQVYASVNKIKNLGQQLYIESMTKSSFKPV TGTRTEPNGGGECKTFTSFHQTLKNNSVYTEPCEDLRNDEHSPSYQQINCIDSVIRYLKSYNIPALKRKC ISCTNTTSSSSEEDKQNHKADDVQALQAGLQIPAIPKSEMPTNGRSIDTGGGAPQILSTAMLSLGSGISQ CGYSSTIVHVPPPETARDATLFCEPWTLNMQPAPLTSEEFKHVGLTAAVLSAHTQKEEQNYVDKFREKIL SSPYSSYLQQESRSKAKYSYFQGDSTSKQTRSAGCRKGKHKRKKLPEPPDSSSSNTGSGPRRGAHQNAQP CCPSAASSPHTSSPTFPPAAMVPSQAPYLVPAFPLPAATSPGREYAAPGTAPEGLHGLPLSEGLQPYPAF PFPYLDTFMTVFLPDPPVCPLLSPSFLPCPFLGATASSAISPSMSSAMSPTLDPPPSVTSQRREEEKWEA QSEGHPFITSRSSSPLQLNLLQEEMPRPSESPDQMRRNTCPQTEYCVTGNNGSESSPATTGALSTGSPPR ENPSHPTASALSTGSPPMKNPSHPTASTLSMGLPPSRTPSHPTATVLSTGSPPSESPSRTGSAASGSSDS SIYLTSSVYSSKISQNGQQSQDVQKKETFPNVAEEPIWRMIRQTPERILMTYQVPERVKEVVLKEDLEKL ESMRQQQPQFSHGQKEELAKVYNWIQSQTVTQEIDIQACVTCENEDSADGAATSCGQVLVEDSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289863
ORF Size	3552 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289863.3
RefSeq Size	6258 bp
RefSeq ORF	3555 bp
Locus ID	8863
UniProt ID	P56645
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Circadian rhythm - mammal
MW	130.6 kDa
Gene Summary	This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.