ATP7A (NM_001282224) Human Tagged ORF Clone
CAT#: RC240136
- TrueORF®
ATP7A (myc-DDK-tagged) - Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A), transcript variant 2
ORF Plasmid: tGFP
"NM_001282224" in other vectors (2)
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CNY 14,160.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DSMAX; MK; MNK; SMAX3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC240136 representing NM_001282224
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTATGGGTGTGAATTCTGTTACCATTTCTGTTGAGGGTATGACTTGCAATTCCTGTGTTT GGACCATTGAGCAGCAGATTGGAAAAGTGAATGGTGTGCATCACATTAAGGTATCACTGGAAGAAAAAAA TGCAACTATTATTTATGACCCTAAACTACAGACTCCAAAGACCCTACAGGAAGCTATTGATGACATGGGC TTTGATGCTGTTATCCATAATCCTGACCCTCTCCCTGTTTTAACTGACACCTTGTTTCTGACTGTTACGG CGTCACTGACTTTGCCATGGGACCATATCCAAAGCACATTGCTGAAGACCAAGGGTGTGACAGACATTAA AATTTACCCTCAGAAAAGAACTGTAGCAGTGACAATAATCCCTTCTATAGTGAATGCCAATCAGATAAAA GAGCTGGTTCCAGAACTCAGTTTAGATACTGGGACACTGGAGAAAAAGTCAGGAGCTTGTGAAGATCATA GTATGGCTCAAGCTGGTGAAGTCGTGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAG CACTATTGAAGGAAAAATTGGGAAACTGCAAGGTGTTCAGCGAATTAAAGTCTCCCTGGACAATCAAGAA GCTACTATTGTTTATCAACCTCATCTTATCTCAGTAGAGGAAATGAAAAAGCAGATTGAAGCTATGGGCT TTCCAGCATTTGTCAAAAAGCAGCCCAAGTACCTCAAATTGGGAGCTATTGATGTAGAACGTCTAAAGAA CACACCAGTTAAATCCTCAGAAGGGTCACAGCAAAGGAGTCCATCATATACCAATGATTCAACAGCCACT TTCATCATTGATGGCATGCATTGTAAATCATGTGTGTCAAATATTGAAAGTACTTTATCTGCACTCCAAT ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCTGCCATTGTGAAGTATAATGCAAGCTCAGTCAC TCCAGAATCCCTGAGAAAAGCAATAGAGGCTGTATCACCGGGGCTATATAGAGTTAGTATCACAAGTGAA GTTGAGAGTACCTCAAACTCTCCCTCCAGCTCATCTCTTCAGAAGATTCCTTTGAATGTAGTTAGCCAGC CTCTGACACAAGAAACTGTGATAAACATTGATGGCATGACTTGTAATTCCTGTGTGCAGTCTATTGAGGG TGTCATATCAAAAAAGCCAGGTGTAAAATCCATACGAGTCTCCCTTGCAAATAGCAATGGGACTGTTGAG TATGATCCTCTACTAACCTCTCCAGAAACGTTGAGAGGAGCAATAGAAGACATGGGATTTGATGCTACCT TGTCAGACACGAATGAGCCGTTGGTAGTAATAGCTCAGCCTTCATCGGAAATGCCGCTTTTGACTTCAAC TAATGAATTTTATACTAAAGGGATGACACCAGTTCAAGACAAGGAGGAAGGAAAGAATTCATCTAAGTGT TACATACAGGTCACTGGCATGACTTGCGCTTCCTGTGTAGCAAACATTGAACGGAATTTAAGGCGGGAAG AAGGAATATATTCTATACTTGTGGCCCTGATGGCTGGCAAGGCAGAAGTAAGGTATAATCCTGCTGTTAT ACAACCCCCAATGATAGCAGAGTTCATCCGAGAACTTGGATTTGGAGCCACTGTGATAGAAAATGCTGAT GAAGGAGATGGTGTTTTGGAACTTGTTGTGAGGGGAATGACGTGTGCCTCCTGCGTACATAAAATAGAGT CTAGTCTCACAAAACACAGAGGGATCCTATACTGCTCCGTGGCCCTGGCAACCAACAAAGCACATATTAA ATATGACCCAGAAATTATTGGTCCTAGAGATATTATCCATACAATTGAAAGCTTAGGTTTTGAAGCTTCT TTGGTCAAGAAGGATCGGTCAGCAAGTCACTTAGATCATAAACGAGAAATAAGACAATGGAGACGGTCTT TTCTTGTGAGTCTGTTTTTCTGTATTCCTGTAATGGGGCTGATGATATATATGATGGTTATGGACCACCA CTTTGCAACTCTTCACCATAATCAAAACATGAGTAAAGAAGAAATGATCAACCTTCATTCTTCTATGTTC CTGGAGCGCCAGATTCTTCCAGGATTGTCTGTTATGAATTTGCTGTCCTTTTTATTGTGTGTACCTGTAC AGGGCAAAACATCAGAGGCTCTTGCAAAGTTAATTTCACTACAAGCTACAGAAGCAACTATTGTAACTCT TGATTCTGATAATATCCTCCTCAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATT AAAGTAGTTCCAGGAGGCAAATTTCCAGTGGATGGTCGTGTTATTGAAGGACATTCTATGGTAGATGAGT CCCTCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAGCACAGTGATTGCTGGTTCCATTAA CCAGAACGGGTCACTGCTTATCTGCGCAACACATGTTGGAGCAGACACAACCCTTTCTCAAATTGTCAAA CTTGTGGAAGAGGCACAAACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTATTTTG TTCCTTTTATTGTTTTTGTTTCCATTGCCACCCTCTTGGTATGGATTGTAATTGGATTTCTGAATTTTGA AATTGTGGAAACCTACTTTCCTGGCTACAATAGAAGTATCTCCCGAACAGAAACGATAATACGATTTGCT TTCCAAGCCTCTATCACAGTTCTGTGTATTGCATGTCCCTGTTCACTGGGACTGGCCACTCCAACTGCTG TGATGGTGGGTACAGGAGTAGGTGCTCAAAATGGCATACTAATAAAAGGTGGAGAGCCATTGGAGATGGC TCATAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATTACTCACGGAACCCCAGTGGTGAATCAA GTAAAGGTTCTAACTGAAAGTAACAGAATATCACACCATAAAATCTTGGCCATTGTGGGAACTGCTGAAA GTAACAGTGAACACCCTCTAGGAACAGCCATAACCAAATATTGCAAACAGGAGCTGGACACTGAAACCTT GGGTACCTGCATAGATTTCCAGGTTGTGCCAGGCTGTGGTATTAGCTGTAAAGTCACCAATATTGAAGGC TTGCTACATAAGAATAACTGGAATATAGAGGACAATAATATTAAAAATGCATCCCTGGTTCAAATTGATG CCAGTAATGAACAGTCATCAACTTCGTCTTCCATGATTATTGATGCCCAGATCTCAAATGCTCTTAATGC TCAGCAGTATAAAGTCCTCATTGGTAACCGGGAGTGGATGATTAGAAATGGTCTTGTCATTAATAACGAT GTAAATGATTTCATGACTGAACATGAGAGAAAAGGTCGGACTGCTGTATTAGTAGCAGTTGATGATGAGC TGTGTGGCTTGATAGCCATTGCAGACACAGTGAAGCCTGAAGCAGAACTGGCTATCCATATTCTGAAATC TATGGGCTTAGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACAGCTAGATCTATTGCTTCTCAGGTT GGCATTACTAAGGTGTTTGCTGAAGTTCTACCTTCTCACAAGGTTGCTAAAGTGAAGCAACTTCAAGAGG AGGGGAAACGGGTAGCAATGGTGGGAGATGGAATCAATGACTCCCCAGCTCTGGCAATGGCTAATGTGGG AATTGCTATTGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGATGTGGTTTTGATAAGGAATGATCTT CTGGATGTAGTGGCAAGTATTGACTTATCAAGAAAGACAGTCAAGAGGATTCGGATAAATTTTGTCTTTG CTCTAATTTATAATCTGGTTGGAATTCCCATAGCTGCTGGAGTTTTTATGCCCATTGGTTTGGTTTTGCA GCCCTGGATGGGATCTGCAGCAATGGCTGCTTCATCTGTTTCTGTAGTACTTTCTTCTCTCTTCCTTAAA CTTTACAGGAAACCAACTTACGAGAGTTATGAACTGCCTGCCCGGAGCCAGATAGGACAGAAGAGTCCTT CAGAAATCAGCGTTCATGTTGGAATAGATGATACCTCAAGGAATTCTCCTAAACTGGGTTTGCTGGACCG GATTGTTAATTATAGCAGAGCCTCTATAAACTCACTACTGTCTGATAAACGCTCCCTAAACAGTGTTGTT ACCAGTGAACCTGACAAGCACTCACTCCTGGTGGGAGACTTCAGGGAAGATGATGACACTGCATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240136 representing NM_001282224
Red=Cloning site Green=Tags(s) MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMG FDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIK ELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQE ATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTAT FIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSE VESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVE YDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKC YIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENAD EGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEAS LVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMF LERQILPGLSVMNLLSFLLCVPVQGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDII KVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVK LVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISRTETIIRFA FQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQ VKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEG LLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINND VNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQV GITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLIRNDL LDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSVVLSSLFLK LYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVV TSEPDKHSLLVGDFREDDDTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282224 |
ORF Size | 4266 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282224.1, NP_001269153.1 |
RefSeq Size | 8306 bp |
RefSeq ORF | 4269 bp |
Locus ID | 538 |
UniProt ID | Q04656 |
Protein Families | Druggable Genome, Transmembrane |
MW | 154.8 kDa |
Gene Summary | This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
SDS |