NUP153 (NM_001278209) Human Tagged ORF Clone
CAT#: RC240167
- TrueORF®
NUP153 (myc-DDK-tagged) - Human nucleoporin 153kDa (NUP153), transcript variant 1
ORF Plasmid: tGFP
"NM_001278209" in other vectors (2)
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CNY 15,010.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HNUP153; N153 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC240167 representing NM_001278209
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGGAGCCGGAGGAGTCGGAGGGGGCGGTGGCGGCAAGATCCGGACGCGGCGTTGCCACCAGG GGCCAATTAAGCCTTACCAGCAGGGGCGACAACAGCATCAGGGCATTCTTAGCAGGGTTACAGAATCTGT TAAGAATATTGTGCCAGGGTGGCTACAAAGATACTTCAACAAGAATGAAGATGTATGCAGCTGTTCAACA GACACAAGCGAGGTTCCACGCTGGCCAGAAAATAAAGAGGACCATCTGGTATATGCCGATGAGGAGAGCT CTAATATTACTGATGGGAGAATCACACCTGAGCCAGCAGTCAGTAATACAGAAGAACCTTCAACAACTAG TACTGCTTCAAATTATCCAGATGTGTTAACAAGGCCTTCTCTTCATCGGAGCCATCTGAATTTTTCCATG TTGGAATCCCCTGCATTACACTGTCAGCCATCTACATCCTCGGCATTCCCAATTGGCAGTTCGGGATTTT CCCTTGTAAAGGAAATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGGTTT TTCTTCAAGAGCTTCTGATAAAGATATAACTGTTTCAAAGAACACTTCATTGCCACCTCTGTGGTCCCCA GAAGCTGAACGTTCTCACTCACTCTCACAGCACACTGCCACCAGCTCAAAAAAACCAGCATTCAACTTGT CTGCCTTTGGAACACTTTCCCCTTCACTTGGGAATTCTTCAATCCTTAAAACCAGTCAGCTTGGAGATTC TCCTTTTTATCCTGGAAAAACAACATACGGTGGGGCAGCAGCTGCTGTAAGACAGTCTAAACTACGAAAT ACACCTTATCAGGCACCAGTTAGAAGACAAATGAAAGCTAAGCAACTCAGTGCACAATCTTACGGTGTGA CCAGTTCAACAGCTCGGCGAATATTGCAGTCTTTAGAGAAGATGTCAAGCCCTTTAGCGGATGCAAAAAG AATTCCATCCATTGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATAGATATCACAGATTTT CAGGCCAAAAGAGAAAAGGTGGATTCTCAATATCCTCCTGTTCAGAGACTTATGACCCCAAAGCCAGTTT CCATAGCAACAAATCGAAGTGTTTATTTTAAACCATCTCTGACTCCTTCTGGTGAATTCAGGAAGACTAA TCAAAGAATAGATAACAAGTGCAGTACTGGATATGAAAAAAATATGACACCCGGACAAAATAGAGAACAA CGAGAAAGTGGCTTTTCATATCCAAATTTCAGTTTGCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTG GTGGAGGTGGCAAGATGAGACGAGAAAGAACACGCTTTGTTGCTTCTAAACCTCTGGAGGAGGAGAGACA AGGTCTCACCGTATTGCCCAAGCTGATCTCGAGCTCCTGCGCTCAAGCGATCATCCCATCTTGGCCTCTC AAAGTGCTGAGATTACAGGAAATGGAAGTTCCAGTATTACCGAAAATCTCTCTACCGATCACCAGTTCTT CACTGCCTACCTTTAATTTTAGTTCCCCTGAGATCACAACTTCCTCTCCATCACCCATCAATTCGTCTCA AGCATTAACAAACAAGGTACAAATGACCTCTCCGAGCAGCACTGGCAGTCCCATGTTTAAATTTTCATCT CCAATCGTAAAATCTACTGAGGCAAATGTACTACCTCCATCATCTATTGGATTTACATTTAGTGTGCCTG TTGCAAAAACAGCAGAACTTTCTGGTTCTAGTAGTACTTTAGAACCAATTATAAGTAGTTCAGCTCATCA TGTCACTACAGTGAACAGTACAAATTGTAAGAAGACACCACCTGAAGATTGTGAGGGTCCTTTTAGACCT GCAGAAATCCTGAAAGAAGGAAGTGTTCTAGATATTCTGAAAAGCCCTGGTTTCGCATCGCCGAAGATAG ATTCTGTTGCTGCTCAGCCCACCGCAACAAGCCCAGTAGTTTATACAAGACCAGCAATAAGTAGCTTTTC TTCTAGTGGAATTGGGTTTGGGGAGAGTTTAAAAGCTGGGTCATCATGGCAGTGTGATACATGTCTACTC CAGAACAAAGTTACAGACAACAAATGCATAGCCTGTCAAGCAGCAAAATTGTCACCCAGAGATACTGCTA AACAGACTGGAATTGAAACACCAAATAAAAGTGGCAAAACAACTCTTTCTGCATCAGGGACAGGCTTTGG AGACAAATTTAAACCAGTGATAGGCACTTGGGATTGTGATACCTGTTTAGTGCAAAATAAACCTGAAGCA ATAAAATGTGTAGCCTGTGAAACACCGAAACCTGGAACTTGTGTGAAGCGAGCCCTTACATTGACAGTGG TTTCGGAAAGTGCTGAGACTATGACTGCTTCATCTTCCAGCTGCACTGTAACCACTGGTACCTTAGGATT TGGAGATAAATTCAAAAGGCCCATTGGATCTTGGGAGTGTTCAGTATGCTGTGTTTCTAATAATGCAGAA GACAATAAGTGTGTGTCCTGTATGTCTGAGAAACCAGGAAGTTCAGTACCTGCTTCAAGTAGCAGCACTG TACCTGTCTCTCTGCCTTCTGGAGGCTCTCTAGGATTGGAAAAGTTCAAGAAACCCGAGGGAAGCTGGGA CTGTGAATTGTGCCTAGTGCAGAATAAGGCAGACTCTACCAAATGTTTGGCATGTGAAAGTGCAAAGCCA GGCACAAAATCTGGGTTTAAAGGCTTTGACACATCTTCCTCATCTTCGAACTCAGCAGCCTCCTCATCCT TCAAATTTGGTGTCTCATCATCCTCTTCTGGGCCTTCTCAGACTTTAACAAGCACTGGAAATTTTAAATT TGGAGATCAGGGAGGATTCAAAATAGGTGTGTCATCCGATTCTGGGTCTATAAACCCCATGAGTGAAGGC TTTAAATTTTCTAAACCAATAGGAGATTTTAAATTTGGAGTTTCATCTGAATCTAAGCCCGAAGAAGTTA AAAAAGATAGTAAGAATGATAATTTTAAGTTTGGACTTTCTTCTGGTTTAAGCAACCCAGTTTCTTTAAC TCCATTTCAATTTGGGGTATCTAATCTTGGACAGGAAGAAAAGAAAGAGGAACTGCCCAAATCTTCCTCT GCAGGTTTTAGCTTTGGTACAGGTGTTATTAACTCCACCCCTGCTCCTGCTAACACCATAGTGACCTCTG AGAACAAGAGCAGCTTCAACCTTGGAACCATAGAAACCAAGAGTGCTTCAGTGGCTCCTTTCACATGTAA GACATCAGAAGCTAAAAAAGAAGAAATGCCTGCCACCAAAGGAGGATTCTCTTTTGGCAACGTGGAGCCT GCCTCTCTGCCATCTGCCTCAGTGTTTGTTTTGGGAAGGACAGAAGAGAAACAGCAAGAGCCTGTCACTT CTACTTCCCTAGTTTTTGGGAAGAAAGCTGACAATGAAGAGCCAAAGTGTCAACCAGTGTTTTCCTTTGG GAATTCAGAGCAAACCAAAGATGAGAATTCTTCAAAGTCCACATTTAGTTTTAGTATGACAAAACCATCT GAGAAGGAATCTGAACAGCCAGCAAAAGCCACTTTTGCCTTTGGAGCTCAAACTAGTACTACAGCTGATC AAGGTGCAGCAAAGCCAGTTTTTAGTTTCTTGAACAACAGTTCCTCTAGTTCAAGTACACCAGCCACTTC TGCTGGTGGTGGCATATTTGGTAGTTCCACCTCTTCCTCCAATCCACCTGTGGCTACCTTTGTGTTTGGA CAGTCCAGCAATCCTGTGAGCAGCTCTGCCTTTGGTAACACTGCTGAATCCAGCACCTCTCAGTCTTTGC TATTTTCTCAAGATAGCAAACTAGCAACCACATCCAGCACAGGTACAGCTGTCACCCCATTTGTCTTTGG TCCAGGAGCCAGCAGTAATAATACTACCACCTCTGGTTTCGGCTTTGGAGCCACAACCACATCTAGCTCT GCAGGATCCTCCTTTGTATTTGGAACTGGACCCTCAGCACCATCTGCCAGTCCAGCATTTGGTGCTAACC AGACCCCAACATTTGGACAAAGTCAAGGTGCCAGCCAGCCCAATCCCCCAGGCTTTGGATCTATATCATC TTCCACAGCATTATTTCCCACTGGTTCTCAGCCTGCACCACCTACTTTTGGGACAGTGTCAAGCAGTAGC CAGCCCCCTGTGTTTGGACAGCAACCTAGTCAGTCTGCATTTGGCTCTGGAACAACTCCTAATTCTAGTT CGGCTTTCCAGTTTGGCAGCAGCACTACAAATTTCAACTTCACAAACAACAGTCCATCAGGAGTGTTCAC ATTTGGTGCAAATTCTAGCACACCTGCAGCCTCAGCCCAGCCTTCAGGCTCGGGGGGCTTTCCATTTAAC CAGTCTCCAGCAGCATTTACAGTGGGGTCAAATGGGAAAAATGTGTTCTCTTCTTCTGGAACTTCATTCT CTGGTCGCAAGATAAAGACTGCTGTTAGACGCAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240167 representing NM_001278209
Red=Cloning site Green=Tags(s) MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCST DTSEVPRWPENKEDHLVYADEESSNITDGRITPEPAVSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSM LESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSLPPLWSP EAERSHSLSQHTATSSKKPAFNLSAFGTLSPSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQSKLRN TPYQAPVRRQMKAKQLSAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSIVSSPLNSPLDRSGIDITDF QAKREKVDSQYPPVQRLMTPKPVSIATNRSVYFKPSLTPSGEFRKTNQRIDNKCSTGYEKNMTPGQNREQ RESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEERQGLTVLPKLISSSCAQAIIPSWPL KVLRLQEMEVPVLPKISLPITSSSLPTFNFSSPEITTSSPSPINSSQALTNKVQMTSPSSTGSPMFKFSS PIVKSTEANVLPPSSIGFTFSVPVAKTAELSGSSSTLEPIISSSAHHVTTVNSTNCKKTPPEDCEGPFRP AEILKEGSVLDILKSPGFASPKIDSVAAQPTATSPVVYTRPAISSFSSSGIGFGESLKAGSSWQCDTCLL QNKVTDNKCIACQAAKLSPRDTAKQTGIETPNKSGKTTLSASGTGFGDKFKPVIGTWDCDTCLVQNKPEA IKCVACETPKPGTCVKRALTLTVVSESAETMTASSSSCTVTTGTLGFGDKFKRPIGSWECSVCCVSNNAE DNKCVSCMSEKPGSSVPASSSSTVPVSLPSGGSLGLEKFKKPEGSWDCELCLVQNKADSTKCLACESAKP GTKSGFKGFDTSSSSSNSAASSSFKFGVSSSSSGPSQTLTSTGNFKFGDQGGFKIGVSSDSGSINPMSEG FKFSKPIGDFKFGVSSESKPEEVKKDSKNDNFKFGLSSGLSNPVSLTPFQFGVSNLGQEEKKEELPKSSS AGFSFGTGVINSTPAPANTIVTSENKSSFNLGTIETKSASVAPFTCKTSEAKKEEMPATKGGFSFGNVEP ASLPSASVFVLGRTEEKQQEPVTSTSLVFGKKADNEEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPS EKESEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFG QSSNPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSS AGSSFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSS QPPVFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFN QSPAAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278209 |
ORF Size | 4518 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001278209.1, NP_001265138.1 |
RefSeq Size | 6030 bp |
RefSeq ORF | 4521 bp |
Locus ID | 9972 |
UniProt ID | P49790 |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
MW | 157.8 kDa |
Gene Summary | Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
Documents
Product Manuals |
FAQs |
SDS |