TrkB (NTRK2) (NM_001018064) Human Tagged ORF Clone
CAT#: RC600035
- TrueORF®
NTRK2 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens neurotrophic tyrosine kinase, receptor, type 2, transcript variant c, Signal peptide (1-31) plus EC domain (32-430)
"NM_001018064" in other vectors (7)
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CNY 6,032.00
Product images
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CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | DDK-His |
Synonyms | DEE58; EIEE58; GP145-TrkB; OBHD; trk-B; TRKB |
Vector | pCMV6-XL5-DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>RC600035 representing leader sequence plus the extracellular domain region of NM_001018064
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGTCGTCCTGGATAAGGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTCTGCTGGCTGGTTGTGG GCTTCTGGAGGGCCGCTTTCGCCTGTCCCACGTCCTGCAAATGCAGTGCCTCTCGGATCTGGTGCAGCGA CCCTTCTCCTGGCATCGTGGCATTTCCGAGATTGGAGCCTAACAGTGTAGATCCTGAGAACATCACCGAA ATTTTCATCGCAAACCAGAAAAGGTTAGAAATCATCAACGAAGATGATGTTGAAGCTTATGTGGGACTGA GAAATCTGACAATTGTGGATTCTGGATTAAAATTTGTGGCTCATAAAGCATTTCTGAAAAACAGCAACCT GCAGCACATCAATTTTACCCGAAACAAACTGACGAGTTTGTCTAGGAAACATTTCCGTCACCTTGACTTG TCTGAACTGATCCTGGTGGGCAATCCATTTACATGCTCCTGTGACATTATGTGGATCAAGACTCTCCAAG AGGCTAAATCCAGTCCAGACACTCAGGATTTGTACTGCCTGAATGAAAGCAGCAAGAATATTCCCCTGGC AAACCTGCAGATACCCAATTGTGGTTTGCCATCTGCAAATCTGGCCGCACCTAACCTCACTGTGGAGGAA GGAAAGTCTATCACATTATCCTGTAGTGTGGCAGGTGATCCGGTTCCTAATATGTATTGGGATGTTGGTA ACCTGGTTTCCAAACATATGAATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACTAACATTTCATC CGATGACAGTGGGAAGCAGATCTCTTGTGTGGCGGAAAATCTTGTAGGAGAAGATCAAGATTCTGTCAAC CTCACTGTGCATTTTGCACCAACTATCACATTTCTCGAATCTCCAACCTCAGACCACCACTGGTGCATTC CATTCACTGTGAAAGGCAACCCCAAACCAGCGCTTCAGTGGTTCTATAACGGGGCAATATTGAATGAGTC CAAATACATCTGTACTAAAATACATGTTACCAATCACACGGAGTACCACGGCTGCCTCCAGCTGGATAAT CCCACTCACATGAACAATGGGGACTACACTCTAATAGCCAAGAATGAGTATGGGAAGGATGAGAAACAGA TTTCTGCTCACTTCATGGGCTGGCCTGGAATTGACGATGGTGCAAACCCAAATTATCCTGATGTAATTTA TGAAGATTATGGAACTGCAGCGAATGACATCGGGGACACCACGAACAGAAGTAATGAAATCCCTTCCACA GACGTCACTGATAAAACCGGTCGGGAACAT ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600035 representing signal peptide plus the extracellular domain region of NM_001018064
Red=Cloning sites Green= DDK and 6XHIS Tags MSSWIRWHGPAMARLWGFCWLVVGFWRAAFACPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITE IFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDL SELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEE GKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVN LTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDN PTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPST DVTDKTGREH TRSGTRSGDYKDDDDKGSHHHHHH |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001018064 |
ORF Size | 2469 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001018064.1, NP_001018074.1 |
RefSeq Size | 8647 bp |
RefSeq ORF | 2469 bp |
Locus ID | 4915 |
UniProt ID | Q16620 |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
Protein Pathways | MAPK signaling pathway, Neurotrophin signaling pathway |
MW | 47.9 kDa |
Gene Summary | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221838 | NTRK2 (Myc-DDK-tagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 6,024.00 |
|
RC221838L1 | Lenti-ORF clone of NTRK2 (Myc-DDK-tagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 8,424.00 |
|
RC221838L2 | Lenti-ORF clone of NTRK2 (mGFP-tagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 7,700.00 |
|
RC221838L3 | Lenti-ORF clone of NTRK2 (Myc-DDK-tagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 7,700.00 |
|
RC221838L4 | Lenti-ORF clone of NTRK2 (mGFP-tagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 7,700.00 |
|
RG221838 | NTRK2 (tGFP-tagged) - Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 7,624.00 |
|
SC311139 | NTRK2 (untagged)-Human neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant c |
CNY 6,032.00 |