Nectin 2 (NECTIN2) (NM_002856) Human Tagged ORF Clone
CAT#: RG200286
- TrueORF®
PVRL2 (tGFP-tagged) - Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_002856" in other vectors (6)
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CNY 7,088.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CD112; HVEB; PRR2; PVRL2; PVRR2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200286 ORF sequence, codon optimized.
Due to the complexity of NM_002856, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGCCGCTGCCCTCCTGCCGTCGAGATCGCCGCCGACGCCGCTGCTGTGGCCGCTGCTGCTGC TGCTGCTCCTGGAAACCGGAGCCCAGGATGTGCGAGTTCAAGTGCTACCCGAGGTGCGAGGCCAGCTCGG GGGCACCGTGGAGCTGCCGTGCCACCTGCTGCCACCTGTTCCTGGACTGTACATCTCCCTGGTGACCTGG CAGCGCCCAGATGCACCTGCGAACCACCAGAATGTGGCCGCCTTCCACCCTAAGATGGGTCCCAGCTTCC CCAGCCCGAAGCCTGGCAGCGAGCGGCTGTCCTTCGTCTCTGCCAAGCAGAGCACTGGGCAAGACACAGA GGCAGAGCTCCAGGACGCCACGCTGGCCCTCCACGGGCTCACGGTGGAGGACGAGGGCAACTACACTTGC GAGTTTGCCACCTTCCCCAAGGGGTCCGTCCGAGGGATGACCTGGCTCAGAGTCATAGCCAAGCCCAAGA ACCAAGCTGAGGCCCAGAAGGTCACGTTCAGCCAGGACCCTACGACAGTGGCCCTCTGCATCTCCAAAGA GGGCCGCCCACCTGCCCGGATCTCCTGGCTCTCATCCCTGGACTGGGAAGCCAAAGAGACTCAGGTGTCA GGGACCCTGGCCGGAACTGTCACTGTCACCAGCCGCTTCACCTTGGTGCCCTCGGGCCGAGCAGATGGTG TCACGGTCACCTGCAAAGTGGAGCATGAGAGCTTCGAGGAACCAGCCCTGATACCTGTGACCCTCTCTGT ACGCTACCCTCCTGAAGTGTCCATCTCCGGCTATGATGACAACTGGTACCTCGGCCGTACTGATGCCACC CTGAGCTGTGACGTCCGCAGCAACCCAGAGCCCACGGGCTATGACTGGAGCACGACCTCAGGCACCTTCC CGACCTCCGCAGTGGCCCAGGGCTCCCAGCTGGTCATCCACGCAGTGGACAGTCTGTTCAATACCACCTT CGTCTGCACAGTCACCAATGCCGTGGGCATGGGCCGCGCTGAGCAGGTCATCTTTGTCCGAGAAACCCCC AGGGCCTCGCCCCGAGATGTGGGCCCGCTGGTGTGGGGGGCCGTGGGGGGGACACTGCTGGTGCTGCTGC TTCTGGCTGGGGGGTCCTTGGCCTTCATCCTGCTGAGGGTGAGGAGGAGGAGGAAGAGCCCTGGAGGAGC AGGAGGAGGAGCCAGTGGCGACGGGGGATTCTACGATCCGAAAGCTCAGGTGTTGGGAAATGGGGACCCC GTCTTCTGGACACCAGTAGTCCCTGGTCCCATGGAACCAGATGGCAAGGATGAGGAGGAGGAGGAGGAGG AAGAGAAGGCAGAGAAAGGCCTCATGTTGCCTCCACCCCCAGCACTCGAGGATGACATGGAGTCCCAGCT GGACGGCTCCCTCATCTCACGGCGGGCAGTTTATGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200286 representing NM_002856
Red=Cloning site Green=Tags(s) MARAAALLPSRSPPTPLLWPLLLLLLLETGAQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTW QRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTC EFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVS GTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDAT LSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETP RASPRDVGPLVWGAVGGTLLVLLLLAGGSLAFILLRVRRRRKSPGGAGGGASGDGGFYDPKAQVLGNGDP VFWTPVVPGPMEPDGKDEEEEEEEEKAEKGLMLPPPPALEDDMESQLDGSLISRRAVYV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002856 |
ORF Size | 1437 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002856.3 |
RefSeq Size | 2166 bp |
RefSeq ORF | 1440 bp |
Locus ID | 5819 |
UniProt ID | Q92692 |
Domains | ig, IGv, IG |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Adherens junction, Cell adhesion molecules (CAMs) |
Gene Summary | This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200286 | PVRL2 (Myc-DDK-tagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha. Note: ORF is codon optimized |
CNY 5,488.00 |
|
RC200286L1 | Lenti-ORF clone of PVRL2 (Myc-DDK-tagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha. Note: ORF is codon optimized |
CNY 7,888.00 |
|
RC200286L2 | Lenti-ORF clone of PVRL2 (mGFP-tagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha. Note: ORF is codon optimized |
CNY 5,890.00 |
|
RC200286L3 | Lenti-ORF clone of PVRL2 (Myc-DDK-tagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha. Note: ORF is codon optimized |
CNY 7,888.00 |
|
RC200286L4 | Lenti-ORF clone of PVRL2 (mGFP-tagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha. Note: ORF is codon optimized |
CNY 5,890.00 |
|
SC118376 | PVRL2 (untagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha |
CNY 5,488.00 |
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