DDX17 (NM_006386) Human Tagged ORF Clone
CAT#: RG200599
- TrueORF®
DDX17 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1
ORF Plasmid: DDK
"NM_006386" in other vectors (6)
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CNY 6,940.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | P72; RH70 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200599 representing NM_006386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGCCCACCGGCTTTGTAGCCCCGATTCTCTGTGTTTTGCTCCCGTCTCCGACGAGAGAGGCGGCGACGG TGGCGTCTGCGACGGGAGACAGCGCGTCGGAGCGAGAGAGCGCTGCGCCTGCCGCCGCCCCAACAGCGGA GGCGCCGCCGCCATCGGTCGTCACCAGACCGGAGCCGCAGGCCCTCCCGAGCCCGGCCATCCGTGCCCCG CTCCCAGATCTCTATCCTTTTGGGACCATGCGCGGAGGAGGCTTTGGGGACCGGGACCGGGATCGTGACC GTGGAGGATTTGGAGCAAGAGGTGGTGGTGGCCTTCCCCCGAAGAAATTTGGTAATCCTGGGGAGCGTTT GCGTAAAAAAAAGTGGGATTTGAGTGAGCTCCCCAAGTTTGAGAAAAATTTTTATGTGGAACATCCGGAA GTAGCAAGGCTGACACCATATGAGGTTGATGAGCTACGCCGAAAGAAGGAGATTACAGTGAGGGGGGGAG ATGTTTGTCCTAAACCCGTGTTTGCCTTCCATCATGCTAACTTCCCACAATATGTAATGGATGTGTTGAT GGATCAGCACTTTACAGAACCAACTCCAATTCAGTGCCAGGGATTTCCGTTGGCTCTTAGTGGCCGGGAT ATGGTGGGCATTGCTCAGACTGGCTCTGGGAAGACGTTGGCGTATCTCCTGCCTGCAATTGTTCATATTA ACCACCAGCCATACTTGGAAAGGGGAGATGGCCCAATCTGTCTAGTTCTGGCTCCTACCAGAGAGCTTGC CCAGCAAGTACAGCAGGTGGCCGATGACTATGGCAAATGTTCTAGATTGAAGAGTACTTGTATTTATGGA GGTGCTCCTAAAGGTCCCCAGATTCGAGACTTGGAAAGAGGTGTTGAGATCTGCATAGCCACTCCTGGAC GTCTGATAGATTTCCTGGAGTCAGGAAAGACAAATCTTCGCCGATGTACTTACCTTGTATTGGACGAAGC TGACAGAATGCTTGATATGGGGTTTGAACCCCAGATCCGTAAAATTGTTGACCAAATCAGGCCTGATAGG CAGACACTGATGTGGAGTGCAACCTGGCCAAAAGAAGTAAGACAGCTTGCAGAGGATTTCCTTCGTGATT ACACCCAGATCAACGTAGGCAATCTGGAGTTGAGTGCCAACCACAACATCCTCCAGATAGTGGATGTCTG CATGGAAAGTGAAAAAGACCACAAGTTGATCCAACTAATGGAAGAAATAATGGCTGAAAAGGAAAACAAA ACAATAATATTTGTGGAGACAAAGAGACGCTGTGATGATCTGACTCGAAGGATGCGCAGAGATGGTTGGC CAGCTATGTGTATCCATGGAGACAAGAGTCAACCAGAAAGAGATTGGGTACTTAATGAGTTCCGTTCTGG AAAGGCACCCATCCTTATTGCTACAGATGTAGCCTCCCGTGGGCTAGATGTGGAAGATGTCAAGTTTGTG ATCAACTATGACTATCCAAACAGCTCAGAGGATTATGTGCACCGTATTGGCCGAACAGCCCGTAGCACCA ACAAGGGTACCGCCTATACCTTCTTCACCCCAGGGAACCTAAAACAGGCCAGAGAGCTTATCAAAGTGCT GGAAGAGGCCAATCAGGCTATCAATCCAAAACTGATGCAGCTTGTGGACCACAGAGGAGGCGGCGGAGGC GGGGGTGGTCGTTCTCGTTACCGGACCACTTCTTCAGCCAACAATCCCAATCTGATGTATCAGGATGAGT GTGACCGAAGGCTTCGAGGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAGTGA AACCGATAGAGCTGGTTATGCTAATGGCAGTGGCTATGGAAGTCCAAATTCTGCCTTTGGAGCACAAGCA GGCCAATACACCTATGGTCAAGGCACCTATGGGGCAGCTGCTTATGGCACCAGTAGCTATACAGCTCAAG AATATGGTGCTGGCACTTATGGAGCTAGTAGCACCACCTCAACTGGGAGAAGTTCACAGAGCTCTAGCCA GCAGTTTAGTGGGATAGGCCGGTCTGGGCAGCAGCCACAGCCACTGATGTCACAACAGTTTGCACAGCCT CCGGGAGCTACCAATATGATAGGTTACATGGGGCAGACTGCCTACCAATACCCTCCTCCTCCTCCCCCTC CTCCTCCTTCACGTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200599 representing NM_006386
Red=Cloning site Green=Tags(s) LPTGFVAPILCVLLPSPTREAATVASATGDSASERESAAPAAAPTAEAPPPSVVTRPEPQALPSPAIRAP LPDLYPFGTMRGGGFGDRDRDRDRGGFGARGGGGLPPKKFGNPGERLRKKKWDLSELPKFEKNFYVEHPE VARLTPYEVDELRRKKEITVRGGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRD MVGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQQVADDYGKCSRLKSTCIYG GAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDR QTLMWSATWPKEVRQLAEDFLRDYTQINVGNLELSANHNILQIVDVCMESEKDHKLIQLMEEIMAEKENK TIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLNEFRSGKAPILIATDVASRGLDVEDVKFV INYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGG GGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDRAGYANGSGYGSPNSAFGAQA GQYTYGQGTYGAAAYGTSSYTAQEYGAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQP PGATNMIGYMGQTAYQYPPPPPPPPPSRK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006386 |
ORF Size | 2187 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006386.5 |
RefSeq Size | 4761 bp |
RefSeq ORF | 2190 bp |
Locus ID | 10521 |
UniProt ID | Q92841 |
Domains | DEAD, helicase_C |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200599 | DDX17 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1 |
CNY 5,344.00 |
|
RC200599L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1, Myc-DDK-tagged |
CNY 7,744.00 |
|
RC200599L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1, mGFP tagged |
CNY 8,170.00 |
|
RC200599L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1, Myc-DDK-tagged |
CNY 8,170.00 |
|
RC200599L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1, mGFP tagged |
CNY 7,744.00 |
|
SC108844 | DDX17 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 1 |
CNY 5,344.00 |