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IFT81 (NM_031473) Human Tagged ORF Clone
CAT＃: RG201265
- TrueORF®
IFT81 (tGFP-tagged) - Human intraflagellar transport 81 homolog (Chlamydomonas) (IFT81), transcript variant 2
ORF Plasmid: DDK
"NM_031473" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDV-1; CDV-1R; CDV1; CDV1R; DV1; SRTD19 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201265 representing NM_031473
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGATCAAATTAAATTCATTATGGACAGTCTCAATAAGGAGCCCTTTAGGAAGAACTATAATTTAA TCACGTTTGATTCCTTGGAGCCAATGCAACTATTACAAGTTCTCAGTGATGTTCTGGCTGAGATTGACCC AAAGCAACTTGTGGATATCAGAGAGGAGATGCCAGAGCAGACAGCCAAACGAATGTTGAGCCTTCTTGGT ATTCTTAAGTACAAACCTTCAGGAAATGCCACAGATATGAGTACTTTTCGTCAGGGTTTGGTGATTGGAA GTAAACCTGTAATTTACCCAGTGCTCCACTGGCTTCTTCAGAGGACTAATGAACTGAAGAAAAGAGCATA TTTAGCTCGTTTTTTAATAAAACTTGAGGTACCAAGTGAGTTTCTTCAGGATGAAACTGTGGCTGACACC AATAAACAGTATGAAGAGTTAATGGAAGCCTTTAAAACTTTGCATAAAGAATATGAGCAGCTCAAGATAT CTGGATTTTCTACAGCAGAAATAAGAAAGGATATCAGTGCAATGGAAGAAGAAAAGGATCAGCTCATTAA GAGAGTTGAACATTTGAAGAAAAGGGTTGAGACAGCTCAGAATCATCAATGGATGCTTAAAATAGCAAGG CAACTTCGAGTTGAAAAAGAGAGAGAAGAATATCTTGCACAACAGAAACAGGAACAAAAGAATCAGCTAT TTCATGCAGTGCAAAGATTGCAAAGAGTACAAAACCAGCTGAAAAGCATGCGCCAAGCTGCAGCAGATGC AAAGCCTGAAAGTTTAATGAAGAGGCTAGAGGAGGAGATAAAATTTAATTTATATATGGTAACTGAAAAA TTTCCTAAAGAATTAGAAAATAAGAAAAAGGAATTACATTTTTTACAAAAAGTAGTTTCAGAGCCAGCTA TGGGCCATTCTGATCTTCTTGAACTTGAATCTAAAATAAATGAAATAAACACAGAAATTAACCAGTTGAT TGAAAAGAAAATGATGAGAAATGAGCCCATTGAAGGCAAACTCTCACTGTATAGGCAACAGGCATCTATC ATTTCCCGTAAAAAAGAAGCCAAAGCTGAGGAACTTCAGGAGGCCAAGGAGAAGTTAGCCAGCCTAGAGA GAGAAGCATCAGTAAAGAGAAATCAGACCCGTGAATTTGATGGTACTGAAGTTTTAAAGGGAGATGAGAG ACAGGATCTCACTCTGTCACCCAGGCTGGAGTGCGGTGGTGTGATCATGGCTTACTGCAGCCTGAAACTC CTAGGCTCAAGTGATCCTCCTACCTCAGCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201265 representing NM_031473
Red=Cloning site Green=Tags(s) MSDQIKFIMDSLNKEPFRKNYNLITFDSLEPMQLLQVLSDVLAEIDPKQLVDIREEMPEQTAKRMLSLLG ILKYKPSGNATDMSTFRQGLVIGSKPVIYPVLHWLLQRTNELKKRAYLARFLIKLEVPSEFLQDETVADT NKQYEELMEAFKTLHKEYEQLKISGFSTAEIRKDISAMEEEKDQLIKRVEHLKKRVETAQNHQWMLKIAR QLRVEKEREEYLAQQKQEQKNQLFHAVQRLQRVQNQLKSMRQAAADAKPESLMKRLEEEIKFNLYMVTEK FPKELENKKKELHFLQKVVSEPAMGHSDLLELESKINEINTEINQLIEKKMMRNEPIEGKLSLYRQQASI ISRKKEAKAEELQEAKEKLASLEREASVKRNQTREFDGTEVLKGDERQDLTLSPRLECGGVIMAYCSLKL LGSSDPPTSAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031473 |
| ORF Size | 1293 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031473.4 |
| RefSeq Size | 2906 bp |
| RefSeq ORF | 1296 bp |
| Locus ID | 28981 |
| UniProt ID | Q8WYA0 |
| Gene Summary | The protein encoded by this gene, together with IFT74, forms a tubulin-binding module of intraflagellar transport complex B. This module is involved in transport of tubulin within the cilium, and the encoded protein is required for ciliogenesis. Mutations in this gene are a cause of short-rib polydactyly syndromes. [provided by RefSeq, Dec 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201265 | IFT81 (Myc-DDK-tagged)-Human intraflagellar transport 81 homolog (Chlamydomonas) (IFT81), transcript variant 2 |
CNY 3,656.00 |
|
| RC201265L3 | Lenti ORF clone of Human intraflagellar transport 81 homolog (Chlamydomonas) (IFT81), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201265L4 | Lenti ORF clone of Human intraflagellar transport 81 homolog (Chlamydomonas) (IFT81), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC107602 | IFT81 (untagged)-Human intraflagellar transport 81 homolog (Chlamydomonas) (IFT81), transcript variant 2 |
CNY 3,656.00 |
