G protein beta 4 (GNB4) (NM_021629) Human Tagged ORF Clone

CAT#: RG201651

  • TrueORF®

GNB4 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), beta polypeptide 4 (GNB4)

ORF Plasmid: DDK tGFP



  "NM_021629" in other vectors (5)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-GNB4 Antibody - middle region
    • 50 ug

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CMTD1F
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG201651 representing NM_021629
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGAACTGGAACAGTTGAGGCAAGAAGCAGAACAACTGCGGAATCAGATTCAGGATGCTCGGAAAG
CATGTAATGATGCAACGCTTGTTCAGATTACATCAAATATGGACTCCGTGGGTCGAATACAAATGCGAAC
AAGACGTACACTGAGGGGCCACCTAGCTAAAATCTATGCTATGCATTGGGGATACGATTCCAGGCTGCTA
GTCAGTGCTTCTCAAGATGGAAAATTAATTATTTGGGATAGCTATACAACAAATAAGATGCATGCTATTC
CTTTGAGGTCCTCCTGGGTGATGACCTGTGCTTATGCTCCCTCTGGTAATTATGTTGCCTGTGGAGGCTT
GGACAACATCTGCTCTATATATAACTTAAAGACCAGAGAGGGAAATGTGAGAGTAAGCCGAGAGTTGCCA
GGTCACACAGGGTACTTGTCCTGCTGTCGTTTTTTAGATGACAGCCAAATTGTTACAAGTTCAGGAGATA
CAACTTGTGCTTTATGGGACATCGAAACTGCCCAGCAGACCACCACATTCACTGGGCATTCTGGAGATGT
GATGAGTCTTTCTTTGAGTCCTGACATGAGGACTTTTGTTTCTGGTGCTTGTGATGCCTCTTCCAAATTA
TGGGATATTCGAGATGGAATGTGTAGACAGTCTTTCACGGGACATGTCTCAGATATCAATGCTGTCAGTT
TTTTCCCAAATGGATATGCCTTCGCCACTGGCTCTGATGATGCCACTTGCCGGCTCTTTGACCTTCGTGC
AGATCAAGAGTTATTATTGTATTCTCATGACAATATCATCTGTGGAATCACTTCTGTAGCCTTCTCAAAA
AGTGGGCGTCTCTTGTTGGCTGGTTACGATGACTTTAATTGTAATGTATGGGACACGCTAAAAGGAGATC
GTGCAGGTGTCCTTGCTGGTCATGACAACCGTGTGAGCTGCTTAGGTGTAACTGATGATGGCATGGCTGT
GGCAACAGGCTCTTGGGACAGTTTTCTTAGAATCTGGAAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG201651 representing NM_021629
Red=Cloning site Green=Tags(s)

MSELEQLRQEAEQLRNQIQDARKACNDATLVQITSNMDSVGRIQMRTRRTLRGHLAKIYAMHWGYDSRLL
VSASQDGKLIIWDSYTTNKMHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELP
GHTGYLSCCRFLDDSQIVTSSGDTTCALWDIETAQQTTTFTGHSGDVMSLSLSPDMRTFVSGACDASSKL
WDIRDGMCRQSFTGHVSDINAVSFFPNGYAFATGSDDATCRLFDLRADQELLLYSHDNIICGITSVAFSK
SGRLLLAGYDDFNCNVWDTLKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLRIWN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021629
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021629.1
RefSeq Size 6428 bp
RefSeq ORF 1023 bp
Locus ID 59345
UniProt ID Q9HAV0
Domains WD40
Protein Families Druggable Genome
Protein Pathways Chemokine signaling pathway
Gene Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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