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POGLUT2 (NM_024089) Human Tagged ORF Clone
CAT＃: RG201660
- TrueORF®
KDELC1 (tGFP-tagged) - Human KDEL (Lys-Asp-Glu-Leu) containing 1 (KDELC1)
ORF Plasmid: DDK
"NM_024089" in other vectors (5)
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CNY 5,336.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EP58; ERp58; KDEL1; KDELC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201660 representing NM_024089
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGCACTTTGCTACTTTATTGCTTCTTTCTGGCGACAGTTCCAGCACTCGCCGAGACCGGCGGAG AAAGGCAGCTGAGCCCGGAGAAGAGCGAAATATGGGGACCCGGGCTAAAAGCAGACGTCGTCCTTCCCGC CCGCTATTTCTATATTCAGGCAGTGGATACATCAGGGAATAAATTCACATCTTCTCCAGGCGAAAAGGTC TTCCAGGTGAAAGTCTCAGCACCAGAGGAGCAATTCACTAGAGTTGGAGTCCAGGTTTTAGACCGAAAAG ATGGGTCCTTCATAGTAAGATACAGAATGTATGCAAGCTACAAAAATCTGAAGGTGGAAGTTAAATTCCA AGGGCAACATGTGGCCAAATCCCCATATATTTTAAAAGGGCCGGTTTACCATGAGAACTGTGACTGTCCT CTGCAAGATAGTGCAGCCTGGCTACGGGAGATGAACTGCCCTGAAACCATTGCTCAGATTCAGAGAGATC TGGCACATTTCCCTGCTGTGGATCCAGAAAAGATTGCAGTAGAAATCCCAAAAAGATTTGGACAGAGGCA GAGCCTATGTCACTACACCTTAAAGGATAACAAGGTTTATATCAAGACTCATGGTGAACATGTAGGTTTT AGAATTTTCATGGATGCCATACTACTTTCTTTGACTAGAAAGGTGAAGATGCCAGATGTGGAGCTCTTTG TTAATTTGGGAGACTGGCCTTTGGAAAAAAAGAAATCCAATTCAAACATCCATCCGATCTTTTCCTGGTG TGGCTCCACAGATTCCAAGGATATCGTGATGCCTACGTACGATTTGACTGATTCTGTTCTGGAAACCATG GGCCGGGTAAGTCTGGATATGATGTCCGTGCAAGCTAACACGGGTCCTCCCTGGGAAAGCAAAAATTCCA CTGCCGTCTGGAGAGGGCGAGACAGCCGCAAAGAGAGACTCGAGCTGGTTAAACTCAGTAGAAAACACCC AGAACTCATAGACGCTGCTTTCACCAACTTTTTCTTCTTTAAACACGATGAAAACCTGTATGGTCCCATT GTGAAACATATTTCATTTTTTGATTTCTTCAAGCATAAGTATCAAATAAATATCGATGGCACTGTAGCAG CTTATCGCCTGCCATATTTGCTAGTTGGTGACAGTGTTGTGCTGAAGCAGGATTCCATCTACTATGAACA TTTTTACAATGAGCTGCAGCCCTGGAAACACTACATTCCAGTTAAGAGCAACCTGAGCGATCTGCTAGAA AAACTTAAATGGGCGAAAGATCACGATGAAGAGGCCAAAAAGATAGCAAAAGCAGGACAAGAATTTGCAA GAAATAATCTCATGGGCGATGACATATTCTGTTATTATTTCAAACTTTTCCAGGAATATGCCAATTTACA AGTGAGTGAGCCCCAAATCCGAGAGGGCATGAAAAGGGTAGAACCACAGACTGAGGACGACCTCTTCCCT TGTACTTGCCATAGGAAAAAGACCAAAGATGAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201660 representing NM_024089
Red=Cloning site Green=Tags(s) MFGTLLLYCFFLATVPALAETGGERQLSPEKSEIWGPGLKADVVLPARYFYIQAVDTSGNKFTSSPGEKV FQVKVSAPEEQFTRVGVQVLDRKDGSFIVRYRMYASYKNLKVEVKFQGQHVAKSPYILKGPVYHENCDCP LQDSAAWLREMNCPETIAQIQRDLAHFPAVDPEKIAVEIPKRFGQRQSLCHYTLKDNKVYIKTHGEHVGF RIFMDAILLSLTRKVKMPDVELFVNLGDWPLEKKKSNSNIHPIFSWCGSTDSKDIVMPTYDLTDSVLETM GRVSLDMMSVQANTGPPWESKNSTAVWRGRDSRKERLELVKLSRKHPELIDAAFTNFFFFKHDENLYGPI VKHISFFDFFKHKYQINIDGTVAAYRLPYLLVGDSVVLKQDSIYYEHFYNELQPWKHYIPVKSNLSDLLE KLKWAKDHDEEAKKIAKAGQEFARNNLMGDDIFCYYFKLFQEYANLQVSEPQIREGMKRVEPQTEDDLFP CTCHRKKTKDEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024089 |
| ORF Size | 1506 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024089.1, NP_076994.1 |
| RefSeq Size | 2082 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 79070 |
| UniProt ID | Q6UW63 |
| Domains | Filamin, CAP10 |
| Gene Summary | This gene encodes a protein product localized to the lumen of the endoplasmic reticulum. As a member of the endoplasmic reticulum protein family the encoded protein contains a Lys-Asp-Glu-Leu or KDEL motif located at the extreme C-terminus which prevents all endoplasmic reticulum resident proteins from being secreted. Proteins carrying this motif are bound by a receptor in the Golgi apparatus so that the receptor-ligand complex returns to the endoplasmic reticulum. A processed non-transcribed pseudogene located in an intron of a sodium transporter gene on chromosome 5 has been defined for this gene. This gene has multiple transcript variants which are predicted to encode distinct isoforms. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201660 | KDELC1 (Myc-DDK-tagged)-Human KDEL (Lys-Asp-Glu-Leu) containing 1 (KDELC1) |
CNY 3,736.00 |
|
| RC201660L3 | Lenti ORF clone of Human KDEL (Lys-Asp-Glu-Leu) containing 1 (KDELC1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC201660L4 | Lenti ORF clone of Human KDEL (Lys-Asp-Glu-Leu) containing 1 (KDELC1), mGFP tagged |
CNY 6,460.00 |
|
| SC112273 | KDELC1 (untagged)-Human KDEL (Lys-Asp-Glu-Leu) containing 1 (KDELC1) |
CNY 7,220.00 |
|
| SC322202 | KDELC1 (untagged)-Human KDEL (Lys-Asp-Glu-Leu) containing 1 (KDELC1) |
CNY 3,744.00 |
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