Spermidine synthase (SRM) (NM_003132) Human Tagged ORF Clone
CAT#: RG201761
- TrueORF®
SRM (tGFP-tagged) - Human spermidine synthase (SRM)
ORF Plasmid: DDK
"NM_003132" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PAPT; SPDSY; SPS1; SRML1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201761 representing NM_003132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGGCCCCGACGGCCCCGCCGCCTCCGGCCCCGCCGCCATCCGCGAGGGCTGGTTCCGCGAGA CCTGCAGCCTGTGGCCCGGCCAGGCCCTGTCGCTGCAGGTGGAGCAGCTGCTCCACCACCGGCGCTCGCG CTACCAGGACATCCTCGTCTTCCGCAGTAAGACCTATGGCAACGTGCTGGTGTTGGACGGTGTCATCCAG TGCACGGAGAGAGACGAGTTCTCCTACCAGGAGATGATCGCCAACCTGCCTCTCTGCAGCCACCCCAACC CGCGAAAGGTGCTGATCATCGGGGGCGGAGATGGAGGTGTCCTGCGGGAGGTGGTGAAGCACCCCTCCGT GGAGTCCGTGGTCCAGTGTGAGATCGACGAGGATGTCATCCAAGTCTCCAAGAAGTTCCTGCCAGGCATG GCCATTGGCTACTCTAGCTCGAAGCTGACCCTACATGTGGGTGACGGTTTTGAGTTCATGAAACAGAATC AGGATGCCTTCGACGTGATCATCACTGACTCCTCAGACCCCATGGGCCCCGCCGAAAGTCTCTTCAAGGA GTCCTATTACCAGCTCATGAAGACAGCCCTCAAGGAAGATGGTGTCCTCTGCTGCCAGGGCGAGTGCCAG TGGCTGCACCTGGACCTCATCAAGGAGATGCGGCAGTTCTGCCAGTCCCTGTTCCCCGTGGTGGCCTATG CCTACTGCACCATCCCCACCTACCCCAGCGGCCAGATCGGCTTCATGCTGTGCAGCAAGAACCCGAGCAC GAACTTCCAGGAGCCGGTGCAGCCGCTGACACAGCAGCAGGTGGCGCAGATGCAGCTGAAGTACTACAAC TCCGACGTGCACCGCGCCGCCTTTGTGCTGCCCGAGTTTGCCCGCAAGGCCCTGAATGATGTGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201761 representing NM_003132
Red=Cloning site Green=Tags(s) MEPGPDGPAASGPAAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQ CTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGM AIGYSSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGVLCCQGECQ WLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYN SDVHRAAFVLPEFARKALNDVS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003132 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003132.2, NP_003123.2 |
RefSeq Size | 1273 bp |
RefSeq ORF | 909 bp |
Locus ID | 6723 |
UniProt ID | P19623 |
Domains | Spermine_synth |
Protein Pathways | Arginine and proline metabolism, beta-Alanine metabolism, Cysteine and methionine metabolism, Glutathione metabolism, Metabolic pathways |
Gene Summary | The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201761 | SRM (Myc-DDK-tagged)-Human spermidine synthase (SRM) |
CNY 2,400.00 |
|
RC201761L1 | Lenti ORF clone of Human spermidine synthase (SRM), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201761L2 | Lenti ORF clone of Human spermidine synthase (SRM), mGFP tagged |
CNY 5,890.00 |
|
RC201761L3 | Lenti ORF clone of Human spermidine synthase (SRM), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201761L4 | Lenti ORF clone of Human spermidine synthase (SRM), mGFP tagged |
CNY 5,890.00 |
|
SC118178 | SRM (untagged)-Human spermidine synthase (SRM) |
CNY 2,400.00 |
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