CCNDBP1 (NM_012142) Human Tagged ORF Clone
CAT#: RG202006
- TrueORF®
CCNDBP1 (tGFP-tagged) - Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1
ORF Plasmid: DDK
"NM_012142" in other vectors (5)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DIP1; GCIP; HHM |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202006 representing NM_012142
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGCAACTGCACCTGCAGCCGCAGTCCCCACCCTGGCTTCGCCTTTGGAGCAGCTCCGGCACT TGGCGGAGGAGCTGCGGTTGCTCCTGCCTCGAGTGCGGGTCGGCGAAGCCCAGGAGACCACCGAGGAGTT TAATCGAGAGATGTTCTGGAGAAGACTCAATGAGGCAGCTGTGACTGTGTCAAGGGAAGCCACGACTCTG ACCATAGTCTTCTCTCAGCTTCCACTGCCGTCTCCACAGGAAACCCAGAAGTTCTGTGAACAAGTCCATG CTGCCATCAAGGCATTTATTGCAGTGTACTATTTGCTTCCAAAGGATCAGGGGATCACCCTGAGAAAGCT GGTACGGGGCGCCACCCTGGACATCGTGGATGGCATGGCTCAGCTCATGGAAGTACTTTCCGTCACTCCA ACTCAGAGCCCTGAGAACAATGACCTTATTTCCTACAACAGTGTCTGGGTTGCGTGCCAGCAGATGCCTC AGATACCAAGAGATAACAAAGCTGCAGCTCTTTTGATGCTGACCAAGAATGTGGATTTTGTGAAGGATGC ACATGAAGAAATGGAGCAGGCTGTGGAAGAATGTGACCCTTACTCTGGCCTCTTGAATGATACTGAGGAG AACAACTCTGACAACCACAATCATGAGGATGATGTGTTGGGGTTTCCCAGCAATCAGGACTTGTATTGGT CAGAGGACGATCAAGAGCTCATAATCCCATGCCTTGCGCTGGTGAGAGCATCCAAAGCCTGCCTGAAGAA AATTCGGATGTTAGTGGCAGAGAATGGGAAGAAGGATCAGGTGGCACAGCTGGATGACATTGTGGATATT TCTGATGAAATCAGCCCTAGTGTGGATGATTTGGCTCTGAGCATATATCCACCTATGTGTCACCTGACCG TGCGAATCAATTCTGCGAAACTTGTATCTGTTTTAAAGAAGGCACTTGAAATTACAAAAGCAAGTCATGT GACCCCTCAGCCAGAAGATAGTTGGATCCCTTTACTTATTAATGCCATTGATCATTGCATGAATAGAATC AAGGAGCTCACTCAGAGTGAACTTGAATTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202006 representing NM_012142
Red=Cloning site Green=Tags(s) MASATAPAAAVPTLASPLEQLRHLAEELRLLLPRVRVGEAQETTEEFNREMFWRRLNEAAVTVSREATTL TIVFSQLPLPSPQETQKFCEQVHAAIKAFIAVYYLLPKDQGITLRKLVRGATLDIVDGMAQLMEVLSVTP TQSPENNDLISYNSVWVACQQMPQIPRDNKAAALLMLTKNVDFVKDAHEEMEQAVEECDPYSGLLNDTEE NNSDNHNHEDDVLGFPSNQDLYWSEDDQELIIPCLALVRASKACLKKIRMLVAENGKKDQVAQLDDIVDI SDEISPSVDDLALSIYPPMCHLTVRINSAKLVSVLKKALEITKASHVTPQPEDSWIPLLINAIDHCMNRI KELTQSELEL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012142 |
ORF Size | 1080 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012142.5 |
RefSeq Size | 1615 bp |
RefSeq ORF | 1083 bp |
Locus ID | 23582 |
UniProt ID | O95273 |
Gene Summary | This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene. [provided by RefSeq, Apr 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202006 | CCNDBP1 (Myc-DDK-tagged)-Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1 |
CNY 3,656.00 |
|
RC202006L3 | Lenti ORF clone of Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202006L4 | Lenti ORF clone of Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC110316 | CCNDBP1 (untagged)-Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1 |
CNY 3,656.00 |
|
SC322349 | CCNDBP1 (untagged)-Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1 |
CNY 3,656.00 |
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