RRAD (NM_004165) Human Tagged ORF Clone

CAT#: RG202927

  • TrueORF®

RRAD (tGFP-tagged) - Human Ras-related associated with diabetes (RRAD), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_004165" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal anti-RAD antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms RAD; RAD1; REM3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG202927 representing NM_004165
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGAACGGCGGCGGCAGCGGAGCGGGCGGGAGCCGCGGTGGGGGCCAGGAGCGCGAGCGCCGTC
GGGGCAGCACACCCTGGGGCCCCGCCCCGCCGCTGCACCGCCGCAGCATGCCGGTGGACGAGCGCGACCT
GCAGGCGGCGCTGACCCCGGGTGCCCTGACGGCGGCCGCGGCCGGGACGGGGACCCAGGGTCCCAGGCTG
GACTGGCCCGAGGACTCCGAGGACTCGCTCAGCTCAGGGGGCAGCGACTCAGACGAGAGCGTTTACAAGG
TGCTGCTGCTGGGGGCGCCCGGCGTGGGCAAGAGCGCCCTGGCGCGCATCTTCGGCGGTGTGGAGGACGG
GCCTGAAGCAGAGGCAGCAGGGCACACCTATGATCGCTCCATTGTAGTGGACGGAGAAGAGGCATCACTC
ATGGTCTACGACATTTGGGAGCAGGACGGGGGCCGCTGGTTGCCCGGCCACTGCATGGCCATGGGGGATG
CCTATGTCATTGTGTACTCAGTGACGGACAAGGGCAGCTTCGAGAAGGCCTCAGAACTGCGGGTCCAGCT
GCGGCGTGCACGGCAAACAGATGATGTGCCCATCATCCTCGTGGGCAACAAGAGCGACCTGGTGCGCTCT
CGTGAGGTCTCGGTGGATGAGGGCCGGGCCTGCGCGGTGGTCTTTGACTGCAAGTTCATTGAGACATCAG
CGGCATTGCACCACAATGTCCAGGCGCTGTTTGAAGGTGTCGTGCGCCAGATACGCCTGCGCAGGGACAG
CAAAGAAGCCAACGCACGACGGCAAGCAGGCACCCGGAGGCGAGAGAGCCTTGGCAAAAAGGCGAAGCGC
TTCTTGGGCCGCATCGTAGCTCGTAACAGCCGCAAGATGGCCTTTCGCGCCAAATCCAAGTCCTGCCACG
ACCTCTCGGTTCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202927 representing NM_004165
Red=Cloning site Green=Tags(s)

MTLNGGGSGAGGSRGGGQERERRRGSTPWGPAPPLHRRSMPVDERDLQAALTPGALTAAAAGTGTQGPRL
DWPEDSEDSLSSGGSDSDESVYKVLLLGAPGVGKSALARIFGGVEDGPEAEAAGHTYDRSIVVDGEEASL
MVYDIWEQDGGRWLPGHCMAMGDAYVIVYSVTDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRS
REVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEANARRQAGTRRRESLGKKAKR
FLGRIVARNSRKMAFRAKSKSCHDLSVL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004165
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004165.3
RefSeq Size 1443 bp
RefSeq ORF 927 bp
Locus ID 6236
UniProt ID P55042
Domains ras, RAS, RAB
Gene Summary May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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