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fast skeletal Myosin (MYLPF) (NM_013292) Human Tagged ORF Clone
CAT＃: RG203081
- TrueORF®
MYLPF (tGFP-tagged) - Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF)
ORF Plasmid: DDK
"NM_013292" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HUMMLC2B; MLC2B; MRLC2; MYL11 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203081 representing NM_013292
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCCAAGAGGGCCAAGAGAAGGACAGTAGAGGGCGGAAGCTCCAGCGTCTTCTCCATGTTCGACC AGACTCAGATCCAGGAGTTCAAAGAGGCCTTCACTGTGATCGACCAGAACCGTGATGGTATTATAGACAA GGAGGACCTTCGGGACACCTTCGCAGCCATGGGCCGCCTCAATGTGAAGAATGAGGAGTTGGATGCCATG ATGAAGGAAGCCAGCGGTCCCATCAACTTCACCGTCTTCCTGACCATGTTCGGGGAGAAGCTCAAGGGTG CCGACCCTGAGGATGTGATCACCGGAGCCTTCAAGGTCTTGGACCCTGAGGGAAAGGGCACCATCAAGAA GAAGTTCCTGGAGGAGCTGCTGACCACGCAGTGTGACCGCTTCTCCCAGGAGGAGATCAAGAACATGTGG GCGGCCTTCCCCCCCGACGTGGGCGGCAACGTCGACTACAAAAACATCTGCTACGTCATCACGCACGGCG ACGCCAAGGACCAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203081 representing NM_013292
Red=Cloning site Green=Tags(s) MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAM MKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMW AAFPPDVGGNVDYKNICYVITHGDAKDQE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013292 |
| ORF Size | 507 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013292.5 |
| RefSeq Size | 687 bp |
| RefSeq ORF | 510 bp |
| Locus ID | 29895 |
| UniProt ID | Q96A32 |
| Protein Pathways | Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203081 | MYLPF (Myc-DDK-tagged)-Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF) |
CNY 2,400.00 |
|
| RC203081L3 | Lenti ORF clone of Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203081L4 | Lenti ORF clone of Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), mGFP tagged |
CNY 5,890.00 |
|
| SC122791 | MYLPF (untagged)-Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF) |
CNY 2,400.00 |
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