BRMS1 (NM_015399) Human Tagged ORF Clone
CAT#: RG203428
- TrueORF®
BRMS1 (tGFP-tagged) - Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1
ORF Plasmid: DDK
"NM_015399" in other vectors (7)
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CNY 4,000.00
CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203428 representing NM_015399
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTCCAGCCTCCAAGCAAAGACACAGAAGAGATGGAAGCAGAGGGTGATTCTGCTGCTGAGATGA ATGGGGAGGAGGAAGAGAGTGAGGAGGAGCGGAGCGGCAGCCAGACAGAGTCAGAAGAGGAGAGCTCCGA GATGGATGATGAGGACTATGAGCGACGCCGCAGCGAGTGTGTCAGTGAGATGCTGGACCTAGAGAAGCAG TTCTCGGAGCTAAAGGAGAAGTTGTTCAGGGAACGACTGAGTCAGCTGCGGTTGCGGCTGGAGGAAGTGG GGGCTGAGAGAGCCCCTGAATACACGGAGCCCCTTGGGGGGCTGCAGCGGAGCCTCAAGATTCGCATTCA GGTGGCAGGGATCTACAAGGGCTTCTGTCTGGATGTGATCAGGAATAAGTACGAATGTGAGCTGCAGGGA GCCAAACAGCACCTGGAGAGTGAGAAGCTGCTGCTCTATGACACGCTGCAGGGGGAGCTGCAGGAGCGGA TCCAGAGGCTGGAGGAGGACCGCCAGAGCCTGGATCTCAGCTCTGAATGGTGGGACGACAAACTGCACGC CAGAGGCAGCTCCAGGTCTTGGGACTCCCTGCCGCCCAGCAAGAGGAAGAAGGCACCTCTGGTTTCTGGC CCATACATCGTGTACATGCTTCAAGAGATCGACATCCTGGAGGACTGGACAGCCATCAAAAAGGCTAGGG CAGCTGTGTCCCCTCAGAAGAGAAAATCGGATGGACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203428 representing NM_015399
Red=Cloning site Green=Tags(s) MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQ FSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQG AKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSG PYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015399 |
ORF Size | 738 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015399.3, NP_056214.1 |
RefSeq Size | 1455 bp |
RefSeq ORF | 741 bp |
Locus ID | 25855 |
UniProt ID | Q9HCU9 |
Protein Families | Druggable Genome |
Gene Summary | This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity
,Kramer, D;Schön, M;Bayerlová, M;Bleckmann, A;Schön, MP;Zörnig, M;Dobbelstein, M;,
Cell Death Dis
,PubMed ID 25675294
[BRMS1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203428 | BRMS1 (Myc-DDK-tagged)-Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1 |
CNY 2,400.00 |
|
RC203428L1 | Lenti ORF clone of Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203428L2 | Lenti ORF clone of Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC203428L3 | Lenti ORF clone of Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203428L4 | Lenti ORF clone of Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC111219 | BRMS1 (untagged)-Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1 |
CNY 3,990.00 |
|
SC321157 | BRMS1 (untagged)-Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1 |
CNY 2,400.00 |