FBXL20 (NM_032875) Human Tagged ORF Clone
CAT#: RG203527
- TrueORF®
FBXL20 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1
ORF Plasmid: DDK
"NM_032875" in other vectors (6)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Fbl2; Fbl20 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203527 representing NM_032875
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGGGACGTGAACGGAGTGACCAAGAGCAGGTTTGAGATGTTCTCAAATAGTGATGAAGCTGTAA TCAATAAAAAACTTCCCAAAGAACTCCTGTTACGGATATTTTCTTTTCTAGATGTTGTTACCCTGTGCCG CTGTGCTCAGGTCTCCAGGGCCTGGAATGTTCTGGCTCTGGATGGCAGTAACTGGCAGCGAATTGACCTA TTTGATTTCCAGAGGGATATTGAGGGCCGAGTAGTGGAGAATATTTCAAAACGATGTGGGGGCTTTTTAC GAAAGTTAAGTCTTCGTGGATGTCTTGGAGTGGGAGACAATGCATTAAGAACCTTTGCACAAAACTGCAG GAACATTGAAGTACTGAATCTAAATGGGTGTACAAAGACAACAGACGCTACATGTACTAGCCTTAGCAAG TTCTGTTCCAAACTCAGGCACCTTGACTTGGCTTCCTGTACATCAATAACAAACATGTCTCTAAAAGCTC TGAGTGAGGGATGTCCACTGTTGGAGCAGTTGAACATTTCCTGGTGTGACCAAGTAACCAAGGATGGCAT TCAAGCACTAGTGAGGGGCTGTGGGGGTCTCAAGGCCTTATTCTTAAAAGGCTGCACGCAGCTAGAAGAT GAAGCTCTCAAGTACATAGGTGCACACTGCCCTGAACTGGTGACTTTGAACTTGCAGACTTGCTTGCAAA TCACAGATGAAGGTCTCATTACTATATGCAGAGGGTGCCATAAGTTACAATCCCTTTGTGCCTCTGGCTG CTCCAACATCACAGATGCCATCCTGAATGCTCTAGGTCAGAACTGCCCACGGCTTAGAATATTGGAAGTG GCAAGATGTTCTCAATTAACAGATGTGGGCTTTACCACTCTAGCCAGGAATTGCCATGAACTTGAAAAGA TGGACCTGGAAGAGTGTGTTCAGATAACAGATAGCACATTAATCCAACTTTCTATACACTGTCCTCGACT TCAAGTATTGAGTCTGTCTCACTGTGAGCTGATCACAGATGATGGAATTCGTCACCTGGGGAATGGGGCC TGCGCCCATGACCAGCTGGAGGTGATTGAGCTGGACAACTGCCCACTAATCACAGATGCATCCCTGGAGC ACTTGAAGAGCTGTCATAGCCTTGAGCGGATAGAACTCTATGACTGCCAGCAAATCACACGGGCTGGAAT CAAGAGACTCAGGACCCATTTACCCAATATTAAAGTCCACGCCTACTTCGCACCTGTCACTCCACCCCCA TCAGTAGGGGGCAGCAGACAGCGCTTCTGCAGATGCTGCATCATCCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203527 representing NM_032875
Red=Cloning site Green=Tags(s) MRRDVNGVTKSRFEMFSNSDEAVINKKLPKELLLRIFSFLDVVTLCRCAQVSRAWNVLALDGSNWQRIDL FDFQRDIEGRVVENISKRCGGFLRKLSLRGCLGVGDNALRTFAQNCRNIEVLNLNGCTKTTDATCTSLSK FCSKLRHLDLASCTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLED EALKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASGCSNITDAILNALGQNCPRLRILEV ARCSQLTDVGFTTLARNCHELEKMDLEECVQITDSTLIQLSIHCPRLQVLSLSHCELITDDGIRHLGNGA CAHDQLEVIELDNCPLITDASLEHLKSCHSLERIELYDCQQITRAGIKRLRTHLPNIKVHAYFAPVTPPP SVGGSRQRFCRCCIIL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032875 |
ORF Size | 1308 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032875.3 |
RefSeq Size | 2423 bp |
RefSeq ORF | 1311 bp |
Locus ID | 84961 |
UniProt ID | Q96IG2 |
Domains | LRR, F-box, LRR_CC |
Protein Families | Druggable Genome |
Gene Summary | Members of the F-box protein family, such as FBXL20, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203527 | FBXL20 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1 |
CNY 3,656.00 |
|
RC203527L1 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC203527L2 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC203527L3 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC203527L4 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
SC320289 | FBXL20 (untagged)-Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1 |
CNY 2,950.00 |