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CHST10 (NM_004854) Human Tagged ORF Clone
CAT＃: RG203793
- TrueORF®
CHST10 (tGFP-tagged) - Human carbohydrate sulfotransferase 10 (CHST10)
ORF Plasmid: DDK
"NM_004854" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HNK-1ST; HNK1ST |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203793 representing NM_004854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCACCAGTGGCTTCTGCTGGCCGCATGCTTTTGGGTGATTTTCATGTTCATGGTGGCTAGCAAGT TCATCACGTTGACCTTTAAAGACCCAGATGTGTACAGTGCCAAACAGGAGTTTCTGTTCCTGACAACCAT GCCGGAAGTGAGGAAGTTGCCAGAAGAGAAGCACATTCCTGAGGAACTGAAGCCAACTGGGAAGGAGCTT CCAGACAGCCAGCTCGTTCAGCCCCTGGTCTACATGGAGCGCCTGGAACTCATCAGAAACGTCTGCAGGG ATGATGCCCTGAAGAATCTCTCGCACACTCCTGTCTCCAAGTTTGTCCTGGACCGAATATTTGTCTGTGA CAAGCACAAGATTCTTTTCTGCCAGACTCCCAAAGTGGGCAACACCCAGTGGAAGAAAGTGCTGATTGTT CTAAATGGAGCATTTTCTTCCATTGAGGAGATCCCCGAAAACGTGGTGCACGACCACGAGAAGAACGGCC TTCCTCGGCTCTCTTCCTTCAGTGATGCAGAAATTCAGAAGCGATTGAAAACATACTTCAAGTTTTTTAT TGTAAGAGATCCCTTCGAAAGACTTATTTCTGCATTTAAGGATAAATTTGTTCACAATCCCCGGTTTGAG CCTTGGTACAGGCATGAGATTGCTCCTGGCATCATCAGAAAATACAGGAGGAACCGGACAGAGACGCGGG GGATCCAGTTTGAAGATTTCGTGCGCTACCTCGGCGATCCGAACCACAGATGGCTAGACCTTCAGTTTGG GGACCACATCATTCACTGGGTGACGTATGTAGAGCTCTGTGCTCCCTGTGAGATAATGTACAGTGTGATT GGACACCACGAGACCCTGGAGGACGATGCCCCATACATCTTAAAAGAGGCTGGCATTGACCACCTGGTGT CATACCCGACTATCCCTCCGGGCATTACCGTGTATAACAGAACCAAGGTGGAGCACTATTTCCTGGGCAT CAGCAAACGAGACATCCGACGCCTGTATGCCCGTTTCGAAGGGGACTTTAAGCTCTTTGGGTACCAGAAA CCAGACTTTTTGCTAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203793 representing NM_004854
Red=Cloning site Green=Tags(s) MHHQWLLLAACFWVIFMFMVASKFITLTFKDPDVYSAKQEFLFLTTMPEVRKLPEEKHIPEELKPTGKEL PDSQLVQPLVYMERLELIRNVCRDDALKNLSHTPVSKFVLDRIFVCDKHKILFCQTPKVGNTQWKKVLIV LNGAFSSIEEIPENVVHDHEKNGLPRLSSFSDAEIQKRLKTYFKFFIVRDPFERLISAFKDKFVHNPRFE PWYRHEIAPGIIRKYRRNRTETRGIQFEDFVRYLGDPNHRWLDLQFGDHIIHWVTYVELCAPCEIMYSVI GHHETLEDDAPYILKEAGIDHLVSYPTIPPGITVYNRTKVEHYFLGISKRDIRRLYARFEGDFKLFGYQK PDFLLN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004854 |
| ORF Size | 1068 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004854.2 |
| RefSeq Size | 2815 bp |
| RefSeq ORF | 1071 bp |
| Locus ID | 9486 |
| UniProt ID | O43529 |
| Domains | Sulfotransfer2 |
| Protein Families | Transmembrane |
| Gene Summary | This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.[provided by RefSeq, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203793 | CHST10 (Myc-DDK-tagged)-Human carbohydrate sulfotransferase 10 (CHST10) |
CNY 3,656.00 |
|
| RC203793L3 | Lenti ORF clone of Human carbohydrate sulfotransferase 10 (CHST10), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203793L4 | Lenti ORF clone of Human carbohydrate sulfotransferase 10 (CHST10), mGFP tagged |
CNY 5,890.00 |
|
| SC117100 | CHST10 (untagged)-Human carbohydrate sulfotransferase 10 (CHST10) |
CNY 2,950.00 |
|
| SC322257 | CHST10 (untagged)-Human carbohydrate sulfotransferase 10 (CHST10) |
CNY 2,950.00 |
