PARS2 (NM_152268) Human Tagged ORF Clone
CAT#: RG203961
- TrueORF®
PARS2 (tGFP-tagged) - Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_152268" in other vectors (4)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DEE75; EIEE75; MT-PRORS; proRS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203961 representing NM_152268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGCTGCTGACAAGATGCAGAGCATTGCCCGCCCTGGCCACCTGCAGCCGCCAGCTCTCTGGGT ATGTTCCTTGCAGGTTTCACCACTGTGCCCCAAGAAGAGGGCGGCGCCTGCTGCTGTCTCGTGTGTTCCA GCCACAGAACCTTCGGGAAGACCGGGTGCTCTCCCTGCAGGACAAATCTGATGACCTGACCTGTAAGAGC CAGCGGCTGATGCTGCAGGTGGGCCTGATCTACCCAGCAAGCCCCGGCTGTTACCACCTCCTGCCATATA CCGTCCGTGCCATGGAGAAGCTCGTGCGAGTGATAGACCAGGAGATGCAGGCCATCGGGGGCCAGAAAGT CAACATGCCCAGCCTCAGCCCGGCAGAGCTCTGGCAAGCCACCAACCGGTGGGACTTGATGGGCAAAGAG CTGCTAAGACTTAGAGACAGGCATGGCAAGGAATACTGCTTAGGACCAACTCACGAGGAAGCCATTACGG CCTTAATTGCCTCCCAGAAGAAACTGTCCTACAAGCAGCTTCCCTTCCTGCTGTACCAAGTGACAAGGAA GTTTCGGGATGAGCCCAGGCCCCGCTTTGGTCTTCTCCGTGGCCGAGAGTTTTACATGAAGGATATGTAC ACCTTTGACTCCTCCCCAGAGGCTGCCCAGCAGACCTACAGCCTGGTGTGTGATGCCTACTGCAGCCTGT TCAACAAGCTAGGGCTGCCATTTGTCAAGGTCCAGGCCGATGTGGGCACCATCGGGGGCACAGTGTCTCA TGAGTTCCAGCTCCCAGTGGATATTGGAGAGGACCGGCTTGCCATCTGTCCCCGCTGCAGCTTCTCAGCC AACATGGAGACACTAGACTTGTCACAAATGAACTGCCCTGCTTGCCAGGGCCCATTGACTAAAACCAAAG GCATTGAGGTGGGGCACACATTTTACCTGGGTACCAAGTACTCATCCATTTTCAATGCCCAGTTTACCAA TGTCTGTGGCAAACCAACCCTGGCTGAAATGGGGTGCTATGGCTTGGGTGTGACACGGATCTTGGCTGCT GCCATTGAAGTCCTCTCTACAGAAGACTGTGTCCGCTGGCCCAGCCTACTGGCCCCTTACCAAGCCTGCC TCATCCCCCCTAAGAAGGGCAGTAAGGAGCAGGCGGCCTCCGAGCTCATAGGGCAGCTGTACGACCACAT CACAGAGGCAGTGCCTCAGCTTCACGGGGAGGTGCTCCTGGACGACAGGACCCATCTGACCATCGGAAAC AGACTGAAAGATGCCAACAAGTTTGGCTACCCCTTTGTGATAATCGCTGGCAAGAGGGCCCTGGAGGACC CTGCACATTTTGAGGTTTGGTGTCAGAACACTGGTGAGGTGGCCTTCCTCACCAAAGATGGAGTCATGGA TTTACTGACCCCAGTGCAGACTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203961 representing NM_152268
Red=Cloning site Green=Tags(s) MEGLLTRCRALPALATCSRQLSGYVPCRFHHCAPRRGRRLLLSRVFQPQNLREDRVLSLQDKSDDLTCKS QRLMLQVGLIYPASPGCYHLLPYTVRAMEKLVRVIDQEMQAIGGQKVNMPSLSPAELWQATNRWDLMGKE LLRLRDRHGKEYCLGPTHEEAITALIASQKKLSYKQLPFLLYQVTRKFRDEPRPRFGLLRGREFYMKDMY TFDSSPEAAQQTYSLVCDAYCSLFNKLGLPFVKVQADVGTIGGTVSHEFQLPVDIGEDRLAICPRCSFSA NMETLDLSQMNCPACQGPLTKTKGIEVGHTFYLGTKYSSIFNAQFTNVCGKPTLAEMGCYGLGVTRILAA AIEVLSTEDCVRWPSLLAPYQACLIPPKKGSKEQAASELIGQLYDHITEAVPQLHGEVLLDDRTHLTIGN RLKDANKFGYPFVIIAGKRALEDPAHFEVWCQNTGEVAFLTKDGVMDLLTPVQTV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_152268 |
ORF Size | 1425 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152268.4 |
RefSeq Size | 2364 bp |
RefSeq ORF | 1428 bp |
Locus ID | 25973 |
UniProt ID | Q7L3T8 |
Protein Pathways | Aminoacyl-tRNA biosynthesis |
Gene Summary | This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of proline to tRNA molecules. Mutations have been found in this gene in some patients with Alpers syndrome. [provided by RefSeq, Mar 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203961 | PARS2 (Myc-DDK-tagged)-Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
RC203961L3 | Lenti ORF clone of Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203961L4 | Lenti ORF clone of Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
SC123237 | PARS2 (untagged)-Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |