CORO2A (NM_052820) Human Tagged ORF Clone
CAT#: RG203993
- TrueORF®
CORO2A (tGFP-tagged) - Human coronin, actin binding protein, 2A (CORO2A), transcript variant 2
ORF Plasmid: DDK
"NM_052820" in other vectors (4)
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CNY 5,512.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CLIPINB; IR10; WDR2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203993 representing NM_052820
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATGGCACCCCCAGTACCGGAGCTCCAAGTTCCGTCATGTCTTTGGCAAACCAGCCAGCAAGGAGA ACTGCTACGACTCCGTGCCTATCACCCGCAGCGTTCACGACAACCACTTCTGTGCCGTGAACCCCCACTT CATTGCAGTTGTGACTGAGTGTGCTGGTGGAGGGGCCTTCCTCGTCATCCCCCTGCACCAGACAGGGAAG TTGGACCCCCACTACCCAAAAGTCTGCGGGCACAGAGGCAACGTTTTGGATGTCAAGTGGAACCCTTTTG ATGATTTTGAGATCGCCTCCTGTTCTGAAGATGCCACAATTAAGATCTGGAGCATCCCCAAGCAGCTGCT GACCAGGAACCTCACGGCCTACAGGAAGGAACTCGTGGGCCACGCGCGCAGAGTAGGCCTGGTGGAGTGG CACCCCACGGCCGCCAACATCCTCTTCAGTGCTGGCTATGACTACAAGGTGATGATCTGGAACCTGGATA CAAAGGAGTCTGTCATCACAAGCCCCATGAGTACAATTAGCTGTCACCAAGATGTGATCCTCTCCATGTC CTTCAACACCAACGGCAGCCTGTTGGCCACCACCTGCAAAGACCGCAAGATTCGGGTTATTGACCCCCGA GCAGGGACCGTCCTCCAGGAGGCCAGCTACAAAGGGCACCGGGCCAGCAAAGTGCTGTTTCTGGGGAACC TGAAGAAGCTGATGTCCACAGGCACATCCCGATGGAACAACCGGCAGGTGGCCTTGTGGGACCAGGATAA CCTCTCTGTGCCTCTGATGGAGGAGGACCTGGACGGCTCCTCGGGCGTGCTGTTTCCCTTCTATGACGCG GACACCAGCATGCTCTACGTGGTGGGGAAGGGAGATGGCAACATCCGCTACTACGAGGTGAGCGCCGACA AGCCTCACCTGAGCTACCTGACTGAGTACCGCTCCTATAACCCACAGAAGGGGATCGGTGTCATGCCAAA GAGAGGACTCGACGTGTCCTCCTGCGAGATCTTCCGCTTCTACAAGCTGATCACAACCAAAAGCCTCATC GAGCCCATCTCCATGATTGTGCCCCGGCGGTCAGAATCCTACCAAGAGGACATATACCCTCCAACAGCAG GGGCCCAGCCCTCCCTGACGGCCCAGGAGTGGCTCAGCGGGATGAATCGAGACCCAATCCTGGTGTCCCT TAGGCCTGGCTCTGAGCTGCTGAGACCCCACCCACTGCCTGCAGAGAGACCTATCTTCAATTCCATGGCC CCAGCCTCACCCCGGCTCTTGAATCAGACAGAAAAGCTGGCTGCAGAAGATGGCTGGAGGTCTTCCTCCC TGTTGGAGGAGAAGATGCCAAGGTGGGCAGCAGAACACAGGCTGGAGGAGAAGAAAACCTGGCTGACAAA TGGCTTTGACGTTTTCGAATGCCCCCCACCAAAGACAGAGAATGAGTTGCTGCAGATGTTCTACCGGCAA CAGGAGGAGATCCGAAGGCTCCGGGAGCTGTTGACCCAGCGAGAGGTCCAGGCCAAACAGTTGGAACTGG AGATCAAAAACTTGCGGATGGGCTCAGAGCAGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203993 representing NM_052820
Red=Cloning site Green=Tags(s) MSWHPQYRSSKFRHVFGKPASKENCYDSVPITRSVHDNHFCAVNPHFIAVVTECAGGGAFLVIPLHQTGK LDPHYPKVCGHRGNVLDVKWNPFDDFEIASCSEDATIKIWSIPKQLLTRNLTAYRKELVGHARRVGLVEW HPTAANILFSAGYDYKVMIWNLDTKESVITSPMSTISCHQDVILSMSFNTNGSLLATTCKDRKIRVIDPR AGTVLQEASYKGHRASKVLFLGNLKKLMSTGTSRWNNRQVALWDQDNLSVPLMEEDLDGSSGVLFPFYDA DTSMLYVVGKGDGNIRYYEVSADKPHLSYLTEYRSYNPQKGIGVMPKRGLDVSSCEIFRFYKLITTKSLI EPISMIVPRRSESYQEDIYPPTAGAQPSLTAQEWLSGMNRDPILVSLRPGSELLRPHPLPAERPIFNSMA PASPRLLNQTEKLAAEDGWRSSSLLEEKMPRWAAEHRLEEKKTWLTNGFDVFECPPPKTENELLQMFYRQ QEEIRRLRELLTQREVQAKQLELEIKNLRMGSEQL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_052820 |
ORF Size | 1575 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_052820.2, NP_438171.1 |
RefSeq Size | 2265 bp |
RefSeq ORF | 1578 bp |
Locus ID | 7464 |
UniProt ID | Q92828 |
Domains | WD40 |
Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 5 WD repeats, and has a structural similarity with actin-binding proteins: the D. discoideum coronin and the human p57 protein, suggesting that this protein may also be an actin-binding protein that regulates cell motility. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203993 | CORO2A (Myc-DDK-tagged)-Human coronin, actin binding protein, 2A (CORO2A), transcript variant 2 |
CNY 3,912.00 |
|
RC203993L3 | Lenti ORF clone of Human coronin, actin binding protein, 2A (CORO2A), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC203993L4 | Lenti ORF clone of Human coronin, actin binding protein, 2A (CORO2A), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
SC109892 | CORO2A (untagged)-Human coronin, actin binding protein, 2A (CORO2A), transcript variant 2 |
CNY 3,920.00 |