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RGS14 (NM_006480) Human Tagged ORF Clone
CAT＃: RG204139
- TrueORF®
RGS14 (tGFP-tagged) - Human regulator of G-protein signaling 14 (RGS14)
ORF Plasmid: DDK
"NM_006480" in other vectors (7)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204139 representing NM_006480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGGAAGCCCAAGCACCTGGGCGTCCCCAACGGGCGCATGGTTCTGGCTGTGTCAGATGGAGAGC TGAGCAGCACGACGGGGCCCCAGGGCCAGGGCGAGGGCCGCGGCAGCTCTCTCAGCATCCACAGCCTCCC CAGTGGTCCCAGCAGCCCCTTCCCAACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCTGTCCTTCGAGCGG CTGTTGCAGGACCCGCTGGGCCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAGTTCAGCGCGGAAAACG TGACTTTCTGGAAGGCCTGCGAGCGCTTCCAGCAGATCCCGGCCAGCGATACCCAGCAGCTAGCTCAGGA GGCCCGCAACATCTACCAGGAGTTCCTGTCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGTCAGGCC TGGCTTGGCGAGGAGGTGCTGGCCGAGCCCCGGCCGGACATGTTTCGGGCACAGCAGCTTCAGATCTTCA ACTTGATGAAGTTCGACAGCTATGCGCGCTTCGTCAAGTCCCCGCTGTACCGCGAGTGCCTGCTAGCCGA AGCCGAGGGACGCCCTCTGCGGGAACCTGGCTCCTCGCGCCTCGGCAGCCCTGACGCCACGAGGAAGAAG CCGAAGCTGAAGCCCGGGAAGTCGCTGCCGCTGGGTGTGGAGGAGTTGGGGCAGCTGCCACCCGTTGAGG GTCCTGGGGGCCGCCCTCTCCGCAAGTCCTTCCGCCGGGAGCTGGGCGGGACTGCAAACGCCGCCTTGCG CCGAGAGTCTCAGGGCTCCCTCAACTCCTCCGCCAGCCTGGACCTTGGCTTCCTAGCCTTCGTCAGCAGC AAATCTGAGAGCCACCGGAAGAGCCTTGGGAGCACGGAGGGTGAAAGTGAAAGCCGGCCAGGGAAGTACT GCTGTGTGTACCTGCCCGATGGCACAGCCTCCTTGGCCCTGGCCAGACCTGGCCTCACCATCCGAGACAT GCTGGCAGGGATCTGTGAGAAACGAGGCCTCTCTCTACCTGACATCAAGGTCTACCTGGTGGGCAATGAA CAGGCCCTGGTCCTGGATCAGGACTGCACCGTGCTGGCGGATCAGGAAGTGCGGCTGGAAAACAGGATCA CCTTCGAGCTGGAGCTGACGGCGCTGGAGCGCGTGGTACGAATCTCAGCCAAGCCCACCAAGCGGCTGCA GGAGGCGCTGCAGCCCATTCTGGAGAAGCACGGCTTGAGCCCGCTAGAGGTGGTGCTGCACCGGCCAGGC GAGAAACAGCCTCTGGATCTGGGGAAGCTAGTGAGCTCGGTGGCGGCCCAGAGACTGGTTTTGGACACTC TTCCAGGTGTGAAGATCTCCAAAGCCCGTGACAAATCTCCCTGCCGCAGCCAGGGCTGCCCACCTAGAAC TCAGGATAAGGCCACCCATCCCCCTCCAGCGTCCCCCAGTTCTCTGGTGAAGGTGCCCAGTAGTGCCACT GGAAAGCGGCAGACCTGTGACATCGAAGGCCTGGTGGAGCTGCTGAACCGGGTGCAGAGCAGCGGGGCCC ACGACCAGAGGGGCCTTCTGAGGAAAGAGGACCTGGTACTTCCAGAATTTCTGCAGCTGCCCGCCCAAGG GCCCAGCTCCGAGGAGACCCCACCACAGACCAAATCAGCAGCCCAGCCCATCGGGGGATCCTTGAACTCC ACCACCGACTCAGCCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204139 representing NM_006480
Red=Cloning site Green=Tags(s) MPGKPKHLGVPNGRMVLAVSDGELSSTTGPQGQGEGRGSSLSIHSLPSGPSSPFPTEEQPVASWALSFER LLQDPLGLAYFTEFLKKEFSAENVTFWKACERFQQIPASDTQQLAQEARNIYQEFLSSQALSPVNIDRQA WLGEEVLAEPRPDMFRAQQLQIFNLMKFDSYARFVKSPLYRECLLAEAEGRPLREPGSSRLGSPDATRKK PKLKPGKSLPLGVEELGQLPPVEGPGGRPLRKSFRRELGGTANAALRRESQGSLNSSASLDLGFLAFVSS KSESHRKSLGSTEGESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGNE QALVLDQDCTVLADQEVRLENRITFELELTALERVVRISAKPTKRLQEALQPILEKHGLSPLEVVLHRPG EKQPLDLGKLVSSVAAQRLVLDTLPGVKISKARDKSPCRSQGCPPRTQDKATHPPPASPSSLVKVPSSAT GKRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPAQGPSSEETPPQTKSAAQPIGGSLNS TTDSAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006480 |
| ORF Size | 1698 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006480.5 |
| RefSeq Size | 2418 bp |
| RefSeq ORF | 1701 bp |
| Locus ID | 10636 |
| UniProt ID | O43566 |
| Domains | RGS, GoLoco, RBD |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204139 | RGS14 (Myc-DDK-tagged)-Human regulator of G-protein signaling 14 (RGS14) |
CNY 4,216.00 |
|
| RC204139L1 | Lenti ORF clone of Human regulator of G-protein signaling 14 (RGS14), Myc-DDK-tagged |
CNY 6,616.00 |
|
| RC204139L2 | Lenti ORF clone of Human regulator of G-protein signaling 14 (RGS14), mGFP tagged |
CNY 5,890.00 |
|
| RC204139L3 | Lenti ORF clone of Human regulator of G-protein signaling 14 (RGS14), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204139L4 | Lenti ORF clone of Human regulator of G-protein signaling 14 (RGS14), mGFP tagged |
CNY 5,890.00 |
|
| SC116059 | RGS14 (untagged)-Human regulator of G-protein signaling 14 (RGS14) |
CNY 4,224.00 |
|
| SC324464 | RGS14 (untagged)-Human regulator of G-protein signaling 14 (RGS14) |
CNY 4,224.00 |
