PYROXD1 (NM_024854) Human Tagged ORF Clone

CAT#: RG204614

  • TrueORF®

PYROXD1 (tGFP-tagged) - Human pyridine nucleotide-disulphide oxidoreductase domain 1 (PYROXD1)

ORF Plasmid: DDK tGFP



  "NM_024854" in other vectors (4)

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CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-PYROXD1 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms MFM8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG204614 representing NM_024854
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCAGCGCGCCCTCCCCCGACGGCAGGGAAGTTCGTGGTGGTCGGCGGCGGCATCGCGGGCGTCA
CTTGTGCGGAGCAGTTGGCTACTCACTTTCCATCGGAAGATATTCTCTTGGTAACAGCTTCTCCTGTTAT
TAAAGCAGTTACAAATTTCAAGCAGATTTCTAAAATATTGGAAGAATTCGATGTTGAAGAACAATCAAGT
ACCATGTTAGGAAAACGCTTTCCCAACATTAAGGTTATAGAATCTGGCGTAAAGCAACTGAAGAGTGAAG
AACACTGCATTGTAACAGAAGATGGCAATCAGCACGTATATAAGAAACTCTGTCTGTGTGCTGGAGCTAA
ACCAAAGTTGATATGTGAAGGAAATCCTTATGTATTAGGAATCCGTGATACAGACAGTGCTCAGGAATTT
CAGAAACAGCTTACTAAAGCTAAAAGAATAATGATCATAGGGAACGGTGGTATTGCACTTGAGTTAGTGT
ATGAAATTGAAGGCTGTGAAGTGATTTGGGCCATTAAAGATAAAGCTATAGGGAATACTTTCTTCGATGC
AGGAGCAGCTGAATTCTTGACTTCAAAGCTCATTGCTGAAAAATCAGAGGCTAAAATTGCACATAAAAGA
ACCAGATATACAACTGAAGGAAGGAAAAAGGAAGCTAGAAGCAAATCTAAAGCAGATAATGTAGGAAGTG
CATTGGGACCAGATTGGCATGAAGGCTTGAATCTTAAAGGAACAAAAGAGTTTTCTCATAAGATTCACCT
TGAAACTATGTGTGAAGTAAAGAAAATCTACCTTCAGGATGAGTTTAGAATTTTGAAGAAAAAGTCCTTC
ACTTTTCCAAGAGACCATAAGTCAGTTACAGCTGATACAGAGATGTGGCCTGTCTATGTGGAATTGACCA
ATGAAAAGATATATGGCTGCGATTTCATTGTCAGTGCTACAGGAGTTACACCAAATGTAGAACCTTTTCT
CCATGGTAACAGTTTTGATCTAGGAGAAGATGGTGGCCTGAAAGTGGATGATCATATGCACACATCCCTT
CCTGATATCTATGCTGCCGGTGACATCTGTACTACATCCTGGCAGCTGAGCCCAGTCTGGCAGCAGATGA
GGCTGTGGACCCAGGCTAGACAGATGGGATGGTATGCAGCAAAGTGCATGGCTGCAGCGAGTTCAGGAGA
CTCTATTGACATGGATTTCAGCTTTGAACTGTTTGCTCATGTGACAAAATTTTTTAACTATAAGGTTGTA
CTGCTGGGAAAATACAATGCACAGGGCTTAGGTTCAGATCATGAATTAATGCTGAGATGTACCAAAGGAC
GAGAATACATCAAAGTCGTCATGCAAAATGGACGAATGATGGGAGCTGTCTTAATTGGTGAAACCGATTT
AGAAGAAACATTTGAAAACCTAATCTTAAACCAAATGAATCTTTCATCATATGGAGAAGATCTGCTAGAT
CCAAATATTGATATAGAAGATTATTTTGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG204614 representing NM_024854
Red=Cloning site Green=Tags(s)

MEAARPPPTAGKFVVVGGGIAGVTCAEQLATHFPSEDILLVTASPVIKAVTNFKQISKILEEFDVEEQSS
TMLGKRFPNIKVIESGVKQLKSEEHCIVTEDGNQHVYKKLCLCAGAKPKLICEGNPYVLGIRDTDSAQEF
QKQLTKAKRIMIIGNGGIALELVYEIEGCEVIWAIKDKAIGNTFFDAGAAEFLTSKLIAEKSEAKIAHKR
TRYTTEGRKKEARSKSKADNVGSALGPDWHEGLNLKGTKEFSHKIHLETMCEVKKIYLQDEFRILKKKSF
TFPRDHKSVTADTEMWPVYVELTNEKIYGCDFIVSATGVTPNVEPFLHGNSFDLGEDGGLKVDDHMHTSL
PDIYAAGDICTTSWQLSPVWQQMRLWTQARQMGWYAAKCMAAASSGDSIDMDFSFELFAHVTKFFNYKVV
LLGKYNAQGLGSDHELMLRCTKGREYIKVVMQNGRMMGAVLIGETDLEETFENLILNQMNLSSYGEDLLD
PNIDIEDYFD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024854
ORF Size 1500 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024854.5
RefSeq Size 3171 bp
RefSeq ORF 1503 bp
Locus ID 79912
UniProt ID Q8WU10
Domains pyr_redox
Protein Families Druggable Genome
Gene Summary This gene encodes a nuclear-cytoplasmic pyridine nucleotide-disulphide reductase (PNDR). PNDRs are flavoproteins that catalyze the pyridine nucleotide-dependent reduction of thiol residues in other proteins. The encoded protein belongs to the class I pyridine nucleotide-disulphide oxidoreductase family but lacks the C-terminal dimerization domain found in other family members and instead has a C-terminal nitrile reductase domain. It localizes to the nucleus and to striated sarcomeric compartments. Naturally occurring mutations in this gene cause early-onset myopathy with internalized nuclei and myofibrillar disorganization. A pseudogene of this gene has been defined on chromosome 11. [provided by RefSeq, Apr 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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