LDB2 (NM_001290) Human Tagged ORF Clone
CAT#: RG204722
- TrueORF®
LDB2 (tGFP-tagged) - Human LIM domain binding 2 (LDB2), transcript variant 1
ORF Plasmid: DDK
"NM_001290" in other vectors (6)
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CNY 7,088.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CLIM1; LDB-2; LDB1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204722 representing NM_001290
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCACACCACATGACCCCTTCTATTCTTCTCCTTTCGGCCCATTTTATAGGAGGCATACACCAT ACATGGTACAGCCAGAGTACCGAATCTATGAGATGAACAAGAGACTGCAGTCTCGCACAGAGGATAGTGA CAACCTCTGGTGGGACGCCTTTGCCACTGAATTTTTTGAAGATGACGCCACATTAACCCTTTCATTTTGT TTGGAAGATGGACCAAAGCGATACACTATCGGCAGGACCCTCATCCCCCGTTACTTTAGCACTGTGTTTG AAGGAGGGGTGACCGACCTGTATTACATTCTCAAACACTCGAAAGAGTCATACCACAACTCATCCATCAC GGTGGACTGCGACCAGTGTACCATGGTCACCCAGCACGGGAAGCCCATGTTTACCAAGGTATGTACAGAA GGCAGACTGATCTTGGAGTTCACCTTTGATGATCTCATGAGAATCAAAACATGGCACTTTACCATTAGAC AATACCGAGAGTTAGTCCCGAGAAGCATCCTAGCCATGCATGCACAAGATCCTCAGGTCCTGGATCAGCT GTCCAAAAACATCACCAGGATGGGGCTAACAAACTTCACCCTCAACTACCTCAGGTTGTGTGTAATATTG GAGCCAATGCAGGAACTGATGTCGAGACATAAAACTTACAACCTCAGTCCCCGAGACTGCCTGAAGACCT GCTTGTTTCAGAAGTGGCAGAGGATGGTGGCTCCGCCAGCAGAACCCACAAGGCAACCAACAACCAAACG GAGAAAAAGGAAAAATTCCACCAGCAGCACTTCCAACAGCAGCGCTGGGAACAATGCAAACAGCACTGGC AGCAAGAAGAAGACCACAGCTGCAAACCTGAGTCTGTCCAGTCAGGTACCTGATGTGATGGTGGTAGGAG AGCCAACTCTGATGGGAGGTGAGTTTGGGGACGAGGACGAAAGGCTAATCACTAGATTAGAAAACACGCA ATATGATGCGGCCAACGGCATGGACGACGAGGAGGACTTCAACAATTCACCCGCGCTGGGGAACAACAGC CCGTGGAACAGTAAACCTCCCGCCACTCAAGAGACCAAATCAGAAAACCCCCCACCCCAGGCTTCCCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204722 representing NM_001290
Red=Cloning site Green=Tags(s) MSSTPHDPFYSSPFGPFYRRHTPYMVQPEYRIYEMNKRLQSRTEDSDNLWWDAFATEFFEDDATLTLSFC LEDGPKRYTIGRTLIPRYFSTVFEGGVTDLYYILKHSKESYHNSSITVDCDQCTMVTQHGKPMFTKVCTE GRLILEFTFDDLMRIKTWHFTIRQYRELVPRSILAMHAQDPQVLDQLSKNITRMGLTNFTLNYLRLCVIL EPMQELMSRHKTYNLSPRDCLKTCLFQKWQRMVAPPAEPTRQPTTKRRKRKNSTSSTSNSSAGNNANSTG SKKKTTAANLSLSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQYDAANGMDDEEDFNNSPALGNNS PWNSKPPATQETKSENPPPQASQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001290 |
ORF Size | 1119 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290.5 |
RefSeq Size | 2463 bp |
RefSeq ORF | 1122 bp |
Locus ID | 9079 |
UniProt ID | O43679 |
Domains | LIM_bind |
Gene Summary | The protein encoded by this gene belongs to the LIM-domain binding family. Members of this family are characterized by a conserved nuclear localization sequence, an amino-terminal homodimerization domain and a carboxy-terminal LIM interaction domain. These proteins function as adapter molecules to allow assembly of transcriptional regulatory complexes. Genetic association studies suggest functions for this gene in rhegmatogenous retinal detachment and coronary artery disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204722 | LDB2 (Myc-DDK-tagged)-Human LIM domain binding 2 (LDB2), transcript variant 1 |
CNY 5,488.00 |
|
RC204722L1 | Lenti ORF clone of Human LIM domain binding 2 (LDB2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204722L2 | Lenti ORF clone of Human LIM domain binding 2 (LDB2), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
RC204722L3 | Lenti ORF clone of Human LIM domain binding 2 (LDB2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204722L4 | Lenti ORF clone of Human LIM domain binding 2 (LDB2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC119326 | LDB2 (untagged)-Human LIM domain binding 2 (LDB2), transcript variant 1 |
CNY 5,488.00 |