GNPDA1 (NM_005471) Human Tagged ORF Clone

CAT#: RG204763

  • TrueORF®

GNPDA1 (tGFP-tagged) - Human glucosamine-6-phosphate deaminase 1 (GNPDA1)

ORF Plasmid: DDK tGFP



  "NM_005471" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal GNPDA1 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms GNP1; GNPDA; GNPI; GPI; HLN
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG204763 representing NM_005471
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTCATCATCCTGGAGCACTATTCTCAGGCGAGCGAGTGGGCGGCTAAATACATCAGGAACCGCA
TCATCCAGTTTAACCCAGGGCCAGAGAAGTACTTCACCCTGGGGCTCCCCACTGGGAGTACCCCACTTGG
CTGCTACAAGAAGCTGATTGAATACTATAAGAATGGGGACCTGTCCTTTAAATATGTGAAGACCTTCAAC
ATGGATGAGTACGTGGGCCTTCCTCGAGACCACCCGGAGAGTTACCACTCCTTCATGTGGAACAACTTCT
TCAAGCACATTGACATCCACCCAGAAAACACCCACATTCTGGATGGGAATGCAGTCGACCTACAGGCAGA
ATGTGATGCCTTTGAAGAGAAGATCAAGGCTGCAGGTGGGATCGAGCTATTTGTTGGAGGCATCGGCCCT
GATGGACACATTGCCTTCAACGAGCCAGGCTCCAGTCTGGTGTCCAGGACCCGTGTGAAGACGCTGGCCA
TGGATACCATCCTGGCCAATGCTAGGTTCTTCGATGGAGAACTCACCAAGGTGCCCACCATGGCCTTGAC
GGTGGGGGTGGGCACTGTCATGGATGCTAGAGAGGTGATGATCCTTATCACAGGTGCTCACAAGGCATTT
GCTCTGTACAAGGCCATCGAGGAGGGAGTGAACCACATGTGGACCGTGTCTGCCTTCCAGCAGCATCCCC
GCACCGTGTTTGTGTGTGACGAGGATGCCACCTTGGAGCTGAAAGTGAAGACTGTCAAGTATTTCAAAGG
TTTAATGCTTGTTCATAACAAGTTGGTGGACCCCTTGTACAGTATCAAAGAGAAAGAAACTGAGAAAAGC
CAATCTTCGAAGAAACCATACAGCGAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG204763 representing NM_005471
Red=Cloning site Green=Tags(s)

MKLIILEHYSQASEWAAKYIRNRIIQFNPGPEKYFTLGLPTGSTPLGCYKKLIEYYKNGDLSFKYVKTFN
MDEYVGLPRDHPESYHSFMWNNFFKHIDIHPENTHILDGNAVDLQAECDAFEEKIKAAGGIELFVGGIGP
DGHIAFNEPGSSLVSRTRVKTLAMDTILANARFFDGELTKVPTMALTVGVGTVMDAREVMILITGAHKAF
ALYKAIEEGVNHMWTVSAFQQHPRTVFVCDEDATLELKVKTVKYFKGLMLVHNKLVDPLYSIKEKETEKS
QSSKKPYSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005471
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005471.5
RefSeq Size 2285 bp
RefSeq ORF 870 bp
Locus ID 10007
UniProt ID P46926
Domains Glucosamine_iso
Protein Pathways Amino sugar and nucleotide sugar metabolism, Metabolic pathways
Gene Summary Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]).[supplied by OMIM, Jan 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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