TSFM (NM_005726) Human Tagged ORF Clone
CAT#: RG204979
- TrueORF®
TSFM (tGFP-tagged) - Human Ts translation elongation factor, mitochondrial (TSFM), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: DDK
"NM_005726" in other vectors (4)
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CNY 5,256.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | EFTS; EFTSMT |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204979 representing NM_005726
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGCTGCGGTCGCTGCGCGTGTTTCTGGTCGCGCGGACCGGGAGCTACCCGGCTGGGTCTCTTC TGCGTCAGTCGCCCCAGCCAAGGCACACATTTTATGCTGGGCCCCGTCTGTCTGCCTCGGCCTCCAGCAA GGAGCTCCTCATGAAGCTGCGGCGGAAAACAGGCTACTCCTTTGTAAATTGCAAGAAAGCTCTGGAGACT TGTGGCGGGGACCTCAAACAGGCAGAGATCTGGCTCCACAAGGAGGCCCAGAAGGAGGGCTGGAGCAAAG CTGCCAAGCTCCAAGGGAGGAAGACCAAAGAAGGCCTGATTGGGCTGTTGCAGGAAGGAAACACAACTGT ATTAGTAGAGGTAAACTGTGAGACAGATTTTGTTTCTAGAAATTTAAAATTTCAACTGTTGGTCCAGCAA GTAGCCCTTGGAACCATGATGCATTGTCAGACCCTAAAGGATCAACCCTCTGCATACAGTAAAGTGCAGT GGCTCACGCCTGTAAACCTAGCACTTTGGGAGGCTGAAGCAGGTGGATCACTTGAGGGTTTCTTGAATTC CTCTGAGCTTTCTGGACTTCCAGCTGGGCCTGACAGAGAAGGCTCACTCAAGGATCAGTTGGCTTTAGCA ATTGGAAAACTGGGAGAAAACATGATTCTTAAACGAGCTGCATGGGTGAAGGTGCCATCTGGGTTCTACG TTGGCTCTTATGTCCACGGAGCAATGCAGAGTCCCTCACTTCACAAGCTGGTGCTGGGGAAGTATGGGGC CCTGGTCATCTGTGAGACGTCTGAACAGAAAACAAACCTTGAAGACGTTGGCCGCCGCCTTGGGCAGCAT GTGGTGGGCATGGCCCCCCTCTCTGTTGGCTCCCTGGACGATGAGCCTGGGGGAGAGGCAGAGACTAAGA TGCTGTCCCAGCCGTATTTGCTGGATCCCTCCATTACCTTGGGGCAGTATGTGCAGCCTCAGGGGGTGTC GGTAGTAGACTTTGTGCGGTTTGAATGTGGAGAAGGTGAAGAGGCAGCAGAAACTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204979 representing NM_005726
Red=Cloning site Green=Tags(s) MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALET CGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQ VALGTMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLPAGPDREGSLKDQLALA IGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQH VVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005726 |
ORF Size | 1038 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005726.3 |
RefSeq Size | 1253 bp |
RefSeq ORF | 978 bp |
Locus ID | 10102 |
UniProt ID | P43897 |
Domains | UBA, EF_TS |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204979 | TSFM (Myc-DDK-tagged)-Human Ts translation elongation factor, mitochondrial (TSFM), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,656.00 |
|
RC204979L3 | Lenti ORF clone of Human Ts translation elongation factor, mitochondrial (TSFM), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204979L4 | Lenti ORF clone of Human Ts translation elongation factor, mitochondrial (TSFM), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC319746 | TSFM (untagged)-Human Ts translation elongation factor, mitochondrial (TSFM), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,656.00 |